防止软籽石榴外植体褐变的研究

为降低软籽石榴组培过程中外植体褐化程度,从外植体表面消毒时间、转瓶间隔时间、培养基的硬度、吸附剂的使用方面进行了综合试验。结果表明:表面消毒最适时间为7～9min,10d转瓶1次降低褐化的效果较好;使用硬度为5g/L琼脂的MS培养基,并添加0.1 mg/LNAA+0.1mg/LIBA+1.0mg/L6-BA+30g/L蔗糖+聚乙烯吡咯烷酮(PVP)2g/L可有效控制褐变并获得较高的愈伤诱导率。     

几种先天畸形胎儿肺组织学观察

目的：探讨不同类型畸形对胎儿肺组织发育的影响程度,为畸形胎儿肺组织的发育研究提供形态学方面的资料。方法：解剖取肺组织,常规切片,HE染色,光学显微镜观察。结果：无脑儿肺组织发育明显滞后,并（缺）指胎儿和胸腹联体胎儿肺组织发育无明显差异。结论：神经系统的发育影响肺组织的发育。     

Levels of plasma soluble complement receptor 1 (sCR1) in normal Indian adult population

A decrease in the membrane anchored erythrocyte complement receptor 1 (CR1) is reported as an acquired phenomenon in a number of inflammatory and autoimmune diseases with concomitant rise in soluble CR1 (sCR1) levels in plasma. There is a need to establish the normal range of sCR1 in Indian adults to assess the function and disease association of this protein. The plasma sCR1 levels of 50 healthy individuals have been estimated by an indigenously developed sandwich ELISA. sCR1 levels from 26 patients suffering from nephropathies had also been assayed which was much higher than the normal controls. This observation suggests sCR1 as a potential market for the assessment of disease activity in nephropathies.     

Alcohol induced effects on kidney

After administration ethanol and its metabolites go through kidneys and are excreted into urine, and its content in the urine is higher than that of the blood and the liver. Chronic ethanol administration decreases the renal tubular reabsorption and reduces renal function. Multiple functional abnormalities of renal tubules may be associated with ethanol-induced changes in membrane composition and lipid peroxidation. The vulnerability of the kidney to oxidative damage has been partly attributed to its high content of long-chain polyunsaturated fatty acids. Renal ultra structural abnormalities due to ethanol exposure may be important in the genesis of functional disturbances. Increased oxidative stress and endothelial dysfunction with their complex interrelationships are relevant aspects of atherogenesis in chronic renal failure. Antioxidants, particularly polyphenols are expected to decrease the vulnerability of the kidney to oxidative challenges.     

茶树愈伤组织多倍体诱导

本文介绍一种利用组织培养手段,快速、有效诱导茶树愈伤组织多倍体的方法。     

植物组织细胞培养的研究进展

综述植物组织和细胞培养技术在快速繁殖,花药培养和单倍体育种,胚胎培养,人工种子制造,植物药物及其它生物制品的生产,植物细胞突变体筛选,原生质培养,体细胞杂交等方面取得的进展.指出植物组织细胞培养中存在的主要问题并提出了相应的对策.     

辽东楤木对过度训练大鼠肾自由基代谢的影响

目的：探讨辽东楤木提取物对运动性肾功能氧化损伤的预防作用。方法：Wistar雄性大鼠45只,随机分为三组：正常对照组（NC,n=12）、运动组（EC,n=17、楤木组（AE,n=16）,通过6周递增负荷游泳训练建立大鼠过度训练模型,采用分光光度法等测定各组大鼠肾SOD、GSH和MDA等指标。结果：（1）与NC组相比：EC组大鼠SOD、GSH水平显著下降,P〈0.01;MDA水平显著升高,P〈0.01。（2）与EC组相比：AE组大鼠SOD、GSH水平明显上升,P〈0.05;MDA水平显著下降,P〈0.01。结论：辽东楤木对运动性肾氧化损伤具有一定的预防作用。     

运动与共轭亚油酸对青春期肥胖大鼠内脏脂肪及肝肾功能的影响

目的：探讨运动与CLA对青春期肥胖大鼠内脏脂肪及肝肾功能的影响。方法：高脂饲养大鼠建模7周后,取24只肥胖大鼠随机分成4组,每组6只,分别为对照组、CLA组、运动组、运动＋CLA组。运动方案为跑台坡度0°,速度为21-25 m/min,总运动时间60min/天,5次/周,给药方案为运动后即刻灌喂1.6g/kg剂量的CLA,共持续干预8周。干预结束后麻醉大鼠采集血液及组织,测肝肾功能和内脏脂肪重量。结果：8周干预后,运动组、运动＋CLA组的体重、内脏脂肪重量及体脂率均明显低于对照组、CLA 组（P＜0.01,P＜0.05）,对照组和CLA组无明显差异（P〉0.05）;对照组、运动组、运动＋CLA组谷草转氨酶和谷草丙比数值明显高于CLA组（P＜0.01）,其他组别之间无明显差异（P＞0.05）;运动组、运动＋CLA组血尿素氮和肌酐较对照组、CLA 组有变化,但无明显差异（P＞0.05）。结论：8周运动与 CLA干预可有效降低青春期肥胖大鼠体重、内脏脂肪重量、体脂率,运动结合CLA能显著减少内脏脂肪含量,增加瘦体重,降低体脂率,具有明显的减肥效果。运动和CLA干预有一定的保肝作用,对肾脏功能无明显影响。     

Correlation of matrix metalloproteinase-2, −9, tissue inhibitor-1 of matrix metalloproteinase and CD44 variant 6 in head and neck cancer metastasis

This study aimed to explore the molecular mechanism in tumor invasion and metastasis. The expression of matrix metalloproteinase-2, −9 (MMP-2, MMP-9), tissue inhibitor-1 of matrix metalloproteinase (TIMP-1), cell adhesion molecule 44 variant 6 (CD44v6), HER2/neu and p53 was investigated in 154 patients with head and neck squamous cell carcinoma (SCC) by ABC and ImmunoMax immunohistochemical method. Their clinical relevance and correlation were analysed. The expression of MMP-2, MMP-9, TIMP-1, CD44v6, HER2/neu and p53 was found in cancer cells in 87.01%, 85.71%, 68.18%, 98.05%, 55.19% and 50.65% cases respectively. Linear regression and correlation analysis revealed that there was close positive relationship (P<0.05) between the expression of MMP-2 and MMP-9, TIMP-1 and CD44v6, HER2/neu and MMP-9, MMP-2 and p53. Up-regulation of MMP-2 was accompanied by advanced T stage (P<0.01). There was also a trend of MMP-2 expression being related with tumor metastasis. Increased expression of HER2/neu was found in patients with tumor recurrence (P<0.05). The expression of TIMP-1 was higher in laryngeal cancer than that in pharyngeal cancer, and higher in keratinizing and non-keratinizing SCC than that in basaloid SCC (P<0.05). These findings suggested that MMP-2 and MMP-9, HER2/neu and MMP-9, MMP-2 and p53 had a coordinate function in aggression of tumor; that MMP-2 had a more important function than MMP-9 in tumor invasion and metastasis; and that HER2/neu might serve as a biomarker for poor prognosis in HNSCC. Project supported by Lübeck Medical University, Germany     

几种先天性畸形胎儿肾脏的解剖与组织学观察

目的:探讨先天畸形胎儿肾脏的解剖及组织学结构特征和不同畸形与肾脏的发育关系。方法:应用形态学方法,对先天性畸形胎儿肾脏进行解剖、观察,并制成组织切片,在光学显微镜下观察其组织学结构。结果:三类10例先天性畸形胎儿中,并、缺指畸形和联体畸形胎儿肾脏的大体形态和组织学观察无明显差异,无脑畸形胎儿肾组织结构发育不全。结论:不同先天畸形对胎儿肾脏发育影响不一。