建立广西金属材料理化检测工程技术公共服务平台的设想

广西是有色金属之乡,是铝、稀土、铟、锡、铅、锌等有色金属原材料以及深加工产品的生产大省,随着中国-东盟经济带上升为国家战略,广西加快产业升级,实现产业集聚的要求越来越迫切。而快速的经济发展和迅猛的产业聚集,必然带来巨大的产品检测需求。因此需建立一个检测工程技术公共服务平台,利用工程检测中心先进的理化检测手段,统一为企业提供优质、高效的理化工程检测服务,帮助企业解决制约产业发展的重大工艺技术"瓶颈"问题,降低企业进入成本,实现特色及支柱产业做大做强,更好地建设一个创新型经济产业群。     

金属管浮子流量计智能检测控制系统研制

针对目前国内浮子流量计精度差、技术落后的现状,对传统浮子流量计实施技术改革,研制智能金属管浮子流量计检测控制系统,采用研华工控机IPC610硬件配以KingView组态王应用软件平台开发系统硬件结构和软件功能,实现了数据实时采集与处理,大大提高金属管浮子流量计的检测精度和稳定性。     

污泥热水解产物中重金属对油麦菜生长的影响

研究了污泥热水解产物中四种重金属的形态分布,以及污泥热水解产物对植物生长的影响.结果表明,热水解过程中,Zn、Cu、Cd和Pb有84%～98%转移到固相中,并且在固相中主要以残渣态存在.污泥水解液促进了油麦菜生长,污泥水解泥饼则对油麦菜生长表现出抑制效果.     

搅拌摩擦点焊接头微观组织和力学性能的研究

本文简介了搅拌摩擦点焊设备的研究及其技术原理,并对国内外学者在搅拌摩擦点焊工艺、微观组织和力学性能方面的研究成果进行了归纳和总结。     

Biochemical characterization of the recombinant schistosome tegumental protein SmALDH_312 produced in E. coli and baculovirus expression vector system

BackgroundThe heterologous expression of parasitic proteins is challenging because the sequence composition often differs significantly from host preferences. However, the production of such proteins is important because they are potential drug targets and can be screened for interactions with new lead compounds. Here we compared two expression systems for the production of an active recombinant aldehyde dehydrogenase (SmALDH_312) from Schistosoma mansoni, which causes the neglected tropical disease schistosomiasis.ResultsWe produced SmALDH_312 successfully in the bacterium Escherichia coli and in the baculovirus expression vector system (BEVS). Both versions of the recombinant protein were found to be active in vitro, but the BEVS-derived enzyme showed 3.7-fold higher specific activity and was selected for further characterization. We investigated the influence of Mg²⁺, Ca²⁺ and Mn²⁺, and found out that the specific activity of the enzyme increased 1.5-fold in the presence of 0.5 mM Mg²⁺. Finally, we characterized the kinetic properties of the enzyme using a design-of-experiment approach, revealing optimal activity at pH 7.6 and 41°C.ConclusionsAlthough, E. coli has many advantages, such as rapid expression, high yields and low costs, this system was outperformed by BEVS for the production of a schistosome ALDH. BEVS therefore provides an opportunity for the expression and subsequent evaluation of schistosome enzymes as drug targets.How to cite: Harnischfeger J, Beutler M, Salzig D, et al. Biochemical characterization of the recombinant schistosome tegumental protein SmALDH_312 produced in E. coli and baculovirus expression vector system. Electron J Biotechnol 2021;54. https://doi.org/10.1016/j.ejbt.2021.08.002     

火电厂锅炉受热面金属监督检验数据库系统研究

结合理论与现场检验实践经验,总结电站锅炉受热面常见爆漏原因,建立数据库系统,为火电厂锅炉检修维护提供参考。     

“焊工工艺学”课堂教学优化思考

“焊工工艺学”是焊接专业中一门技术基础课,其教学主要侧重学生对焊接工艺的工艺制定和分析能力的培养,要做好“焊工工艺学”的教学工作,就必须对课堂教学进行优化。应当注重理论的延展性,转变教学方法,提高课堂气氛,从而达到理想的教学效果。     

汽车焊装车间的钢结构设计

为了解决在多种设备林立的焊装车间内的钢结构布置与合理设计,以满足各种承载和吊挂的需求。文章介绍了汽车焊装车间钢结构设计与工艺、机械化输送、公用动力等的密切配合,可供类似设计参考。     

编写高职汽车材料与金属加工教材的几点思考

高职教材的编写有其特殊性。我们在编写汽车材料与金属加工教材时，注意做到以下几点：一、注重内容的实用性和实践性；二、充分考虑高职学生的接受能力，力求简约；三、增加新的现代科技内容，删减缺乏应用性的陈旧内容；四、提供课内外结合的空间；五、改革现行考核方式。