CRP,but not TNF-α or IL-6, decreases after weight loss in patients with morbid obesity exposed to intensive weight reduction and balneological treatment

Objective

The aim of this study was to evaluate the concentrations of C-reactive protein (CRP), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and the degree of homeostasis model assessment-insulin resistance (HOMA-IR) in patients with morbid obesity exposed to a three-week low-calorie diet and balneotherapy.

Methods

The study included 33 patients (25 females and 8 males; mean age 46 years) with body mass index (BMI) values of >40 kg/m². Evaluations of CRP, IL-6, TNF-α, lipid profile, HOMA-IR, and fasting glucose were carried out before (baseline data) and three weeks after the treatment. The control group consisted of 20 healthy volunteers (15 females and 5 males) with a mean age of 39 years and BMI values of ≤24.9 kg/m².

Results

In the blood of patients with morbid obesity we found significantly elevated levels of CRP, TNF-α, triglycerides, HOMA-IR and fasting glucose, but a decreased level of high density lipoprotein (HDL)-cholesterol, compared with the healthy individuals. The treatment resulted in about a 9.4% reduction in body weight from 122.5 to 111.0 kg and a significant decrease in the concentration of CRP, but no change in TNF-α or IL-6. HOMA-IR was significantly reduced.

Conclusion

The decrease in CRP level without changes in TNF-α or IL-6 concentrations after the low-calorie diet and balneological treatment, suggests that an essential amount of adipose tissue must be removed before proper adipocyte function is restored. The decrease in HOMA-IR indicates an improvement in insulin sensitivity, which is beneficial in obese patients.

     

Effect of epidermal growth factor on follicle-stimulating hormone-induced proliferation of granulosa cells from chicken prehierarchical follicles

The development of ovarian follicular cells is controlled by multiple circulating and local hormones and factors, including follicle-stimulating hormone (FSH) and epidermal growth factor (EGF). In this study, the stage-specific effect of EGF on FSH-induced proliferation of granulosa cells was evaluated in the ovarian follicles of egg-laying chickens. Results showed that EGF and its receptor (EGFR) mRNAs displayed a high expression in granulosa cells from the prehierarchical follicles, including the large white follicle (LWF) and small yellow follicle (SYF), and thereafter the expression decreased markedly to the stage of the largest preovulatory follicle. SYF represents a turning point of EGF/EGFR mRNA expression during follicle selection. Subsequently the granulosa cells from SYF were cultured to reveal the mediation of EGF in FSH action. Cell proliferation was remarkably increased by treatment with either EGF or FSH (0.1–100 ng/ml). This result was confirmed by elevated proliferating cell nuclear antigen (PCNA) expression and decreased cell apoptosis. Furthermore, EGF-induced cell proliferation was accompanied by increased mRNA expressions of EGFR, FSH receptor, and the cell cycle-regulating genes (cyclins D1 and E1, cyclin-dependent kinases 2 and 6) as well as decreased expression of luteinizing hormone receptor mRNA. However, the EGF or FSH-elicited effect was reversed by simultaneous treatment with an EGFR inhibitor AG1478. In conclusion, EGF and EGFR expressions manifested stage-specific changes during follicular development and EGF mediated FSH-induced cell proliferation and retarded cell differentiation in the prehierarchical follicles. These expressions thus stimulated follicular growth before selection in the egg-laying chicken.     

便携式大容量太阳能锂离子电池充电器的设计

锂离子电池其高能量、高电压、长寿命、无污染等优势成为人们的首选。硅太阳能电池可分为单晶硅电池、多晶硅电池、非晶硅电池。锂离子电池的充电过程可分为三个阶段即恒流模式、恒压模式及涓流模式。为此我们利用多晶硅电池,非晶硅电池作为主要的充电能源,对锂离子或者锂-聚合物可充电电池通过电池管理芯片,实现可移动太阳能锂离子电池充电。     

胎盘源细胞治疗急性辐射骨髓衰竭研究

核辐射易造成人体造血系统损伤,为此建立急性辐射骨髓衰竭小鼠模型,通过单次腹腔注射异基因小鼠多份不同胎盘源细胞／细胞因子,明确其治疗急性辐射骨髓衰竭的疗效．实验将造模后小鼠随机分为胎盘源细胞治疗组、胎盘源细胞因子治疗组、胎盘源细胞联合细胞因子治疗组以及模型小鼠对照组4组,治疗后统计分析各组小鼠存活时间．结果表明,胎盘源细胞联合细胞因子可有效延缓急性辐射骨髓衰竭小鼠的寿命（P ＜0．05）．     

SYSMEX UF-1000i尿有形成分分析仪对尿路感染的诊断价值

采用Sysmex UF-1000i尿有形成分分析仪和AX-4280尿干化学分析仪测定尿标本,同时进行尿细菌定量培养。把测定值与尿培养金标准比对,干化学法白细胞酯酶和UF-1000i细菌计数结果的一致性适中,与亚硝酸盐结果的一致性较低,与尿白细胞计数结果的一致性极高。细菌定量培养的特异性、灵敏度及一致性高于UF-1000i白细胞计数。UF-1000i在临床快速诊断尿路感染时应用价值较高。     

语言中的“反”性别歧视

语言是社会的镜子,同样也随着时代的脚步而逐步更新,它过滤了违背时代潮流的“旧语”,也推出了顺应时代潮流的“新语”。而语言性别歧视话题几经斗争和演变,也都将会在语言这面镜子中得到修正和完善。传统上人们总是片面地认为性别歧视语言主要针对女性,而忽视了对男性的语言歧视;并且,既然性别歧视语言是在历史发展中产生的,那么也必然会随着历史发展而演变。因此,该文从相反的视角,提出“反”性别歧视---一方面研究女性在语言歧视中的“反抗”,另一方面也探究女性“反过来”针对男性的歧视。     

白藜芦醇联合野生型p53质粒诱导非小细胞肺癌H1299细胞凋亡

目的实验以非小细胞肺癌H1299细胞(简称H1299细胞)为研究对象,主要探讨白藜芦醇联合野生型p53质粒对H1299细胞凋亡作用的影响以及进一步探讨其作用机制。方法在H1299细胞中导入野生型p53质粒,加入不同浓度的白藜芦醇,通过MTT法确定白藜芦醇联合野生型p53质粒的最佳作用浓度,并检测白藜芦醇联合野生型p53质粒对细胞增殖的影响;光镜下观察细胞形态学的变化;流式细胞术检测白藜芦醇联合野生型p53质粒对H1299细胞凋亡率的影响;RT-PCR实验方法检测细胞凋亡相关基因Bcl-2、Bax、Caspase3、Caspase9的表达水平。结果白藜芦醇联合野生型p53质粒能明显抑制H1299细胞的增殖,诱导H1299细胞凋亡,明显抑制Bcl-2基因的表达水平,促进Bax、caspase3、caspase9基因的表达水平。结论白藜芦醇联合野生型p53质粒能够明显抑制H1299细胞的增殖,可能通过促凋亡基因Bax、凋亡蛋白酶Caspase3,Cas?pase9的表达,抑制抑凋亡基因Bcl-2的表达,从而激活细胞中线粒体介导的凋亡通路,诱导H1299细胞凋亡。     

广州市花都区城镇建设用地适宜性评价研究——基于MCR面特征提取

从降低城市化过程中因建设用地扩展所致生态不利影响出发,本文以最小累积阻力(MCR)模型为基础,选取已有建设用地作为扩展源,构建地形、地质、生态脆弱性和可达性四个阻力因子体系。在生成的MCR面上,划分建设用地适宜性分区,并利用Hydrology扩展模块提取建设用地适宜扩展路径、生态隔离带和生态关键点,为城镇生态建设规划和景观格局优化提供决策支持。主要结果包括:应用本文方法分析建设用地适宜性评价的面、线、点综合特征,比仅进行扩展面评价分析的常规方法更为全面,且更具可操作性;划分出建设用地适宜扩展区、较适宜扩展区、限制扩展区、禁止扩展区和生态恢复区,并提出相应的管理措施;建设用地在适宜扩展区和较适宜扩展区内,由扩展路径引导优化开发,可避免盲目地"摊大饼式"蔓延;生态隔离带打破现有建设区"连片"扩展格局;生态关键点和高值点的识别,有利于研究区生态环境保育。     

有氧运动对中老年大鼠骨髓内皮祖细胞增殖能力的影响

目的：探讨有氧运动对中老年大鼠骨髓来源内皮祖细胞增殖能力的影响。方法：健康中年雄性 SD 大鼠12只,按体重随机分为两组,即空白对照组和有氧运动组。采用乳酸阈测定法和递增负荷跑台训练建立有氧运动模型,每周训练6天,为期16周。方案结束后12h 提取大鼠骨髓,运用密度梯度离心法分离得到单个核细胞,以 EGM -2培养诱导其分化获取内皮祖细胞,通过双荧光染色法观察细胞对乙酰化低密度脂蛋白的摄取和与荆豆凝集素1的结合,并结合形态学特征鉴定内皮祖细胞。倒置显微镜下计数贴壁细胞数量和计算集落形成率以反映细胞增殖情况。结果：各组贴壁细胞数量第7d 多于第48h(P <0.01);与对照组相比,有氧运动组贴壁细胞数量明显增加(P <0.01),且集落形成率大(P <0.01)。结论：有氧运动能促进中老年大鼠骨髓来源的内皮祖细胞增殖。