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101.
To establish a method for determining five saponins(notoginsenoside R1, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 and ammonium glycyrrhizinate) in Glycyrrhizae, Notoginseng and Ginseng, the high performance liquid chromatography with diode array detector(HPLC-DAD) method was applied to an Inertsil ODS-SP column(4.6 mm×250 mm, 5 μm) with a mobile phase consisting of acetonitrile-0.05% phosphoric acid in a gradient elution manner. The flow rate was 1.0 mL/min. The column temperature was 30 ℃ and the detection wavelengths were 203 nm and 237 nm, respectively. The linear ranges were 0.700,0—7.000,0 μg for R1(r=1.000,0), 0.751,1— 7.511,4 μg for Rg1(r=1.000,0), 0.677,2—6.771,6 μg for Re(r=1.000,0), 0.733,9—7.339,1 μg for Rb1(r= 1.000,0), and 0.540,0—5.399,8 μg for ammonium glycyrrhizinate(r=0.999,9), respectively. In addition, their average recoveries were 100.28%, 105.83%, 104.09%, 99.36% and 98.54%, respectively. The relative standard deviations(RSDs) of precision, reproducibility and recovery were all less than 1.5%. The results indicate that the method is simple, accurate and reproducible so that it can be used for the simultaneous determination of the five saponins in Chinese patent medicines containing the three kinds of herbs. 相似文献
102.
谭志静 《河南科技学院学报》2007,35(2):41-42
通过实验建立了慈姑中愈创木酚、儿茶酚、焦性没食子酸的含量测定方法,该测定方法快捷、简便、准确,所测结果稳定、重现性好。实验采用ODS柱,柱温35℃,甲醇-水(含1%乙酸,体积比为35:65)溶液为流动相,用紫外检测器于275 nm处检测。结果表明,愈创木酚进样量为0.03μg~1.2μg;进样量与色谱蜂呈良好的线性关系(r=0.998);样品的加标回收率为98%~101%;相对标准偏差(RSD)为1.40%(n=6)。 相似文献
103.
A mathematical model of the dynamic behavior of countercurrent chromatography was proposed, and the model parameters, including the partition coefficient, the axial dispersion coefficient, the intraparticle diffusion coefficient and the external mass ransfer coefficient were calculated by the method of chromatogram moment analysis. Comparison of the experimental chromatograms of caffeine and theophylline determined in this work with the simulated curves computed by the proposed model showed fairly good agreement. Further, the difference between the average identified the partition coefficients by chromatogram moment analysis and the experimental values was small also, and the relationship between the external mass transfer rate and the linear velocity was similar to that obtained with solid-liquid chromatography. 相似文献
104.
E. Raji Nair Swati Banerjee Satish Kumar M. V. R. Reddy B. C. Harinath 《Indian journal of clinical biochemistry : IJCB》2001,16(1):132-135
Proteins secreted into the culture medium byMycobacterium tuberculosis (M. tb) are shown to be source of antigens of immunodiagnostic interest. Anin vitro released 31 kDa antigen ESAS-7F isolated fromM.tb H37Ra culture filtrate by salt precipitation, SDS-PAGE and cation exchange fast protein liquid chromatography (FPLC) was shown
earlier to be a diagnostically important antigen fraction. In this report, we describe the isolation of ESAS-7F antigen using
monospecific antibody coupled to sepharose CL-4B column. The percentage recovery of ESAS-7F antigen using affinity chromatography
was approximately 8% of the total ES antigen proteins compared to 0.05% obtained by conventional purification steps using
salt precipitation, SDS-PAGE and FPLC. Similar seroreactivity was observed by the antigen isolated by both the methods in
indirect ELISA. Affinity chromatography helped in an increased recovery of ESAS-7F antigen and obviates the need for time
consuming conventional purification steps. 相似文献
105.
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107.
本文研究了以硅胶G为吸附剂的薄层色谱法从农药稻瘟灵原油或乳油中分离出主成份1,3—二硫戊环—2—叉—丙二酸二异丙酯(DIDM)的最佳条件,拟定了用紫外分光光度法测定样品中DIDM含量的实用的新方法. 相似文献
108.
气相色谱法测定面粉中过氧化苯甲酰 总被引:2,自引:0,他引:2
陶静 《鞍山师范学院学报》2004,6(2):44-46
面粉中的过氧化苯甲酰经乙醚提取浓缩后,被碘化钾还原反应生成苯甲酸,利用气相色谱法对其进行分离测定,并与标准品比较定性、定量.该方法的最低检出含量为0.3 mg/kg,加标回收率在90%~99%之间.该方法分离效率高,样品用量少,检测灵敏度高. 相似文献
109.
Lipophilic tea polyphenols (LTP) were prepared by catalytic esterification of green tea polyphenols (GTP) with hexadecanoyl
chloride. A novel long-chain acyl-derivative of epigallocatechin-3-o-gallate (EGCG) was first isolated from purification of LTP by high-speed countercurrent chromatography (HSCCC) using a solvent
system composed of n-hexane-ethyl acetate-methanol-water (1∶1∶1∶1, v/v). The molecular structure of the acyl-derivative, Epigallocatechin-3-O-gallate-4′-O-hexadecanate, was elucidated by means of elemental analysis, IR,1H-NMR and MS spectra.
Project (No. 200010) supported by Zhejiang Provincial Natural Science Foundation, China 相似文献
110.