In vitro study on ribozyme against hepatitis C virus

Objective: To determine the effective nucleotide sites of ribozymes against HCV, and obtain a highly effective, nontoxic and inexpensive antisense ribozyme specific for HCV. Methods: Two effective ribozymes, targeted to HCV 5’NC region and C region, were designed and synthesized. Eukaryotic expression vectors, pSV2-gpt. CD-SR (, containing either HCRZNC or HCRZC were constructued and transfected into MT-2 cells, which had been infected by HCV. Quantitative PCR and hydridization were used to determine the effect of inhibition of HCV by ribozymes. Results: HCRZNC and HCRZC suppressed the replication of HCV by 54.7% and 62.1%, respectively. Furthermore, when both ribozymes were cotransfected into cells, they suppressed replication by 78.8%. Conclusion: Two specific antisense ribozymes have strong inhibitory effects on the replication of HCV in cultured cells, and have better effect when used together.     

羟乙基淀粉130/0.4复苏对失血性休克大鼠肺组织NF-κB的影响

目的通过肺组织NF-κB的表达,研究羟乙基淀粉130/0.4复苏对失血性休克大鼠肺损伤保护作用的可能机制.方法健康雄性SD大鼠54只,体重230~280 g,随机分为3组：对照组（C组）、乳酸林格氏液复苏组（RL组）、6%羟乙基淀粉130/0.4复苏组（HES组）,按照Wiggers改良法复制失血性休克模型.于复苏后1 h、2 h4、h采用免疫组织化学的方法检测肺组织NF-κB的表达;光镜下观察肺组织形态学改变.结果 RL组与HES组肺组织NF-κB免疫组化染色细胞核阳性百分率较C组明显升高,RL组升高的程度较HES组明显（P〈0.01）.免疫组化染色光镜观察显示：HES组比RL组的组织损伤明显减轻.结论 6%羟乙基淀粉130/0.4复苏失血性休克可以抑制肺组织NF-κB活性,减轻休克及复苏过程造成的肺损伤.     

基于行动导向开发高职《B/S结构程序开发》课程

以行动导向教学观为指导,从典型工作任务分析出发,确定了职业能力及职业素质目标,详细阐述了教学项目设计、实施、考核的课程设计流程,探讨了高职B/S结构程序开发课程的教学设计和教学实施。     

脊髓亚急性联合变性24例临床分析

目的探讨脊髓亚急性联合变性（SCD）患者的临床特点、诊断及预后。方法回顾性分析24例SCD患者的临床特点、辅助检查结果、治疗及预后。结果 21例血清维生素B12水平降低。17例行脊柱MRI检查,10例有异常信号,呈条带状等T1长T2异常信号。24例患者均给予维生素B12肌肉注射,14例治愈（58.3%）,6例明显好转（25.0%）,4例效果不明显（16.7%）。结论 SCD患者主要表现为脊髓后索、侧索受累,周围神经受累亦较常见。及时应用维生素B12治疗,症状可明显好转。本病为可治性疾病,预后与病程密切相关,应重视早期诊断、治疗。     

基于RUP的C/S和B/S混合模式构建学籍管理系统

在分析了高校学籍管理系统的特点和功能模块的基础上,针对C/S和B/S各自的优缺点,提出了基于RUP的C/S和B/S混合模式的学籍管理系统设计思想;讨论了学籍管理系统的总体结构和功能设计。     

Enhancing cellular immune response to HBV M DNA vaccine in mice by codelivery of interleukin-18 recombinant

Objective: To investigate the effect of interleukin-18 (IL-18) on immune response induced by plasmid encoding hepatitis B virus middle protein antigen and to explore new strategies for prophylactic and therapeutic HBV DNA vaccines. Methods: BALB/c mice were immunized with pCMV-M alone or co-immunized with pcDNA3-18 and pCMV-M and then their sera were collected for analysing anti-HBsAg antibody by ELISA; splenocytes were isolated for detecting specific CTL response and cytokine assay in vitro. Results: The anti-HBs antibody level of mice co-immunized with pcDNA3-18 and pCMV-M was slightly higher than that of mice immunized with pCMV-M alone, but there was not significantly different (P>0.05). Compared with mice injected with pCMV-M, the specific CTL cytotoxity activity of mice immunized with pcDNA3-18 and pCMV-M was significantly enhanced (P<0.05) and the level of IFN-γ in supernatant of splenocytes cultured with HBsAg in vitro was significantly elevated (P<0.05) while the level of IL-4 had no significant difference (P>0.05). Conclusion: The plasmid encoding IL-18 together with HBV M gene DNA vaccines may enhance specific TH1 cells and CTL cellular immune response induced in mice, so that IL-18 is a promising immune adjuvant.     

B2B电子商务模式探讨

文章介绍了电子商务的几种主要模式及B2B电子商务概况，认为B2B模式将成为未来电子商务的主要模式。原因在于B2B模式具有使买卖双方信息交流低廉、快捷，可降低企业间的交易成本，减少企业的库存，缩短企业生产周期等竞争优势。同时还就B2B电子商务模式对企业的影响和制约我国B2B电子商务发展的因素及对策等问题进行了探讨。     

论公共计算机实验室管理系统的开发及使用

随着网络技术高速发展，公共计算机实验室的使用率也呈不断上升的趋势。因此，对公共计算机实验室进行系统而科学的管理已成为公共计算机实验室管理员的当务之急。本系统作为现行公共计算机实验室管理必不可少的组成部分．将实验室的机器资源合理分配，统一管理．提高设备利用率，从而使实验室的教学质量得到有力保证。     

基于B/S体系结构管理信息系统的分析研究

传统的管理信息系统采用的是C/S体系结构,它是20世纪后期最为流行的软件体系结构。随着Inter-net的兴起和网络技术的成熟,更多管理信息系统的体系结构开始向B/S结构转变。B/S结构的系统利用不断成熟和普及的浏览器技术,实现了原来需要复杂的专用软件才能实现的强大功能,极大地降低了运行成本。     

一氧化氮合酶在病毒性肝炎中作用的研究进展

一氧化氮(NO)参与肝细胞的多项生理功能调节,参与病毒性肝炎的病理过程。一氧化氮合酶(NOS)为产生NO的限速酶,与NO的各种生理功能密切相关。本文综述了NOS基因在正常肝组织和病毒性肝炎的不同表达情况及其作用和意义。