拟南芥热激因子AtHsfAla在PET原核表达系统中的表达及纯化

以pErl30a为载体构建的表达拟南芥热激因子AtHsfAla的大肠杆菌EcoliM15（pET30a／His6一AtHsfAla）为实验材料,以IPTG诱导6XHis融合蛋白的表达并经过Ni2＋柱分离纯化AtHsfAla,再通过SDS—PAGE鉴定表达蛋白和纯化蛋白．结果显示,AtHsfAla蛋白在在PET原核表达系统中能够有效表达,并能通过亲和层析获得纯化的AtHsfAla蛋白．研究结果为揭示拟南芥热激因子AtHsfAla的作用机理奠定基础．     

电气化铁路接触网绝缘性能的实践研究

结合同煤集团四台沟电气化铁路110 kv牵引变电所及接触网供电设备故障跳闸率对煤矿电气化铁路定量装车点的接触网设备结构及污染情况进行了系统分析,研究出了提高重污区接触网设备绝缘性能的技改方法,并附注实践取得了良好的效果。     

基于《医疗法规与医患沟通》课程进行“早期接触临床”的教学实践与思考

《医疗法规与医患沟通》课程目标是培养医学生对患者及其家属进行有效沟通的能力和对医疗相关法规理解、掌握并应用的能力,最终获得医疗相应岗位的职业沟通技能和职业法律素养,为将来从事医疗服务工作奠定扎实的基础。＂早期接触临床＂的初衷包括培养医学生与病人的交流沟通能力,这与本课程的开设目的一致。将两者有机结合更有助于培养学生的职业素质,激发学习兴趣,达到教育教学改革的目的 。     

他砂汉语的标记被动式

他砂汉语的标记被动式有"名<,受>着+[名<,施>]+动""名<,受>+名<,施>+各+动""名<,受>着+名<,施>+各+动"等三种类型.讨论这三种标记被动式的用法及被动标志的来源.他砂汉语中由被动标记"各"和"着……各"构成的被动式均与土家语的影响有关,它们在形成过程中经历了土家语的抵抗和改造,属于汉语、土家语深度...     

English Proficiency,Identity, Anxiety,and Intergroup Attitudes: US Americans’ Perceptions of Chinese

Guided by the Common Ingroup Identity Model and Berry’s acculturation framework, this study examined the roles that perceptions of language proficiency, cultural identity, and communication anxiety had on intergroup attitudes and stereotypes in the American–Chinese contact context. Serial mediation analyses with 10,000 bootstrap samples revealed that perceived English proficiency of a Chinese contact had significant indirect effects on affective and behavioral attitudes toward Chinese through American participants’ perceptions of their contact’s host and home culture identification and communication anxiety. Perceived English proficiency had an indirect effect only on positive stereotypes through the Chinese contact’s perceived identification with home culture.     

The majority influence on interminority attitudes: The secondary transfer effect of positive and negative contact

Among minority members, positive contact with the majority was previously found to improve not only the attitudes toward the majority but also the attitudes toward minority outgroups (the secondary transfer effect; STE). However, the roles of negative intergroup contact and minority groups’ social status in the STE have not been yet examined. Therefore, in the present study, we investigated the association between both positive and negative contact with the national majority group (Finns) and mutual attitudes among high-status Estonian (n = 171) and low-status Russian (n = 180) immigrants in Finland. Two mediators of the STE were tested: attitudes toward the majority (attitude generalization) and public collective self-esteem (diagonal hostility). While positive and negative STEs emerging via attitude generalization were expected to occur among both immigrant groups, the mediating effect of public collective self-esteem was assumed only for members of the low-status group. In both immigrant groups, the relationship between positive contact with the majority group and attitudes toward the other immigrant group was positive and indirect through more favorable attitudes toward majority group members. The same mechanism characterized negative contact, where the indirect effect was mediated by less positive attitudes toward Finns. As predicted, public collective self-esteem mediated the effects of positive and negative contact with majority group members on attitudes toward the other minority only among low-status Russian immigrants. The results call for the acknowledgement of different mechanisms explaining the STE among minority groups enjoying different social statuses in host society.     

交叉双尖窄状多重温室与太阳能热利用

将日光温室进一步改造为内部成交叉双尖窄状的多重温室,使尖窄状内温度更高,从而使置于尖窄状内的太阳能集热器的热效率更高,达90%左右,并将这种特殊结构的温室安装在建筑物窗户外面下端的墙壁上,而热水箱则放于室内,并用软管与集热器相连通,就构成一种窗户型太阳能热利用装置.从而"将多重温室太阳能与建筑相结合,并且使用方式从房顶转向窗户",是一条新的技术路线.     

屋顶花园是建筑环保节能的有效途径

屋顶花园的开发利用,可以改善城市阳光产生的辐射热,并加强城市景观与建筑的相互融洽,改善城市环境,保护生态平衡,对屋面产生隔热、保温、减噪作用;但是,屋顶花园在设计和施工中却存在着一些值得探讨和注意的问题.     

气膜厚度对反气泡稳定性的影响

为观察气膜厚度对反气泡稳定性的影响,通过加热反气泡内外溶液来加热气膜内气体从而改变气膜厚度。实验发现:在一定温度范围内,加热反气泡气膜可以延长其稳定时间,但是当加热反气泡气膜使其厚度超过其稳定存在的阈值时,反气泡的稳定性会急剧下降甚至无法产生反气泡;在一定温度范围内温度对溶液表面张力影响很小,这使得文中实验数据合理化。本文为反气泡稳定性的理论研究提供实验支持,并考虑到气膜厚度为微米级,可为微米级气膜传热提供实验素材。     

AMC视角下多市场接触对企业研发强度的影响--基于全球医药行业的实证研究

以往对多市场接触的研究大多集中于考察多市场接触对下游产品市场竞争强度的影响，本文将研究的注意力转向上游要素市场。基于多市场接触理论和动态竞争的意识—动机—能力 (awareness-motivation-capability, AMC) 框架，探讨了多市场接触对企业研发强度的影响。对全球最大的38家制药企业2007—2016年期间的面板数据分析表明，把企业间竞争的范围延伸到上游研发市场时，多市场接触会表现出显著的竞争效应，即多市场接触会正向影响企业的研发强度，从而说明经典的相互克制效应并不适用于要素市场。此外，本文检验了多市场接触和研发强度关系的边界条件，焦点企业专利存量和现金持有比会削弱多市场接触和研发强度之间的正向关系，而销量下降产品比例会强化二者之间关系。