Urinary proteomics as a novel tool for biomarker discovery in kidney diseases

Urine has become one of the most attractive biofluids in clinical proteomics,for its procurement is easy and noninvasive and it contains sufficient proteins and peptides. Urinary proteomics has thus rapidly developed and has been extensively applied to biomarker discovery in clinical diseases,especially kidney diseases. In this review,we discuss two important aspects of urinary proteomics in detail,namely,sample preparation and proteomic tech-nologies. In addition,data mining in urinary proteomics is also b...     

1912年以前湖南的疫灾流行与防治

湖南是一个灾害频发的省份,对人民生产生活和社会发展影响最大的除水旱灾害之外,当数传染病流行造成的疫灾,这是一种直接危害人类健康甚至危及生命,给社会造成巨大损失的灾害.民国以前影响湖南的主要有鼠疫、霍乱、天花、麻诊、伤寒、肝炎、流感、疟疾、血吸虫、痢疾等.疫灾多由水旱虫灾饥馑引发.对于疾疫的救治方式,一为积极的方式,即施药治病;一为消极的方式,即巫术禳灾.近代防疫宣传和措施在城市也许有一定成效,但对广大贫苦人民尤其是农村人口来说,他们处于贫穷和饥饿的境地,首要的问题乃是解决温饱,卫生和防疫不过是一种奢望.他们缺医少药,遇疫仍不免"修斋建醮,乞灵冥漠".     

中专学校传染病的预防和控制

中专学校是一个特殊场所,学生居住环境和学习环境相对集中,容易发生传染病的传播、蔓延和暴发流行,现就中专学校传染病的预防与控制提了几点建议.     

试论太极拳与健身防病

太极拳是一项合乎人体生理和体育原理的健身运动,也是集导引、经络、阴阳学说于一体的运动。文章主要从内景方面阐述太极拳运动健身防病的作用,以便更多的太极拳爱好者从中受益。     

学校体育中运动性疾病的现状与对策

随着学生参与体育活动人数的不断增多,与运动有关的运动性疾病的发生率也有所增多,这对学校深入开展体育运动带来不良影响.笔者通过调查,对目前学校体育中广泛存在的运动性疾病的现状、发生率特点及成因进行科学性系统分析,并根据学校体育运动的实际,提出了相应的预防对策.     

长链非编码RNA（1ncRNAs）的研究策略及其在中枢神经退行性疾病中的研究进展

长链非编码RNA（IncRNAs）是真核细胞中转录本长度超过200nt的非编码RNA。近年来的研究表明lncRNAs具有一系列的重要功能,包括影响转录、RNA剪切、翻译、核因子转运、基因重排及染色质修饰等,lncRNAs的异常表达常与肿瘤、神经退行性疾病、心血管系统疾病等的发生发展密切相关,因此对lncRNAs的研究将有助于对机体生理病理反应的内在机制形成更为系统的认识。该文就lncRNAs的研究策略及其在神经退行性疾病中的研究进展进行了综述,以期为神经系统调控网络的建立及神经退行性疾病的防治提供新的思路。     

“五大支持系统”背景下学生工作的实践探索

学生工作者在繁冗复杂的工作面前要科学地梳理工作。高校辅导员将所带大学生的工作对象中重点人群可分为优秀大学生群体、经济困难大学生群体、沉迷网络学生群体、学业有困难学生群体和疾病、心理问题学生群体,对特殊群体因材施教,以做好特殊群体的教育管理工作,从而实现学生工作的整体覆盖。     

江苏高速公路绿带主要病虫害及防控研究

本文对江苏高速公路绿带主要病虫害进行了调查汇总,介绍了常见病虫害的种类、寄主、危害症状、发生规律并提出相应的有效防治方法,为充分发挥江苏省高速公路绿化作用打下良好的基础。     

Somatic DNA Damages in Cardiovascular Autonomic Neuropathy

Cardiovascular autonomic neuropathy (CAN) is one of the most clinically significant complications of diabetes mellitus. Even though many ethological factors have been attributed for the pathogenesis of this disease no attempts were made to correlate DNA damage as a causative factor. Hence the present study was undertaken to asses the extent of somatic DNA damages by cytokinesis-block micronuclei assay (CBMN). An attempt is also being made to correlate the habits and/or risk factors and socioeconomic status with CAN. The CBMN frequency of 46 patients suffering from autonomic neuropathy was compared with that of 25 healthy age and sex matched controls. All the subjects were suffering from type 2 diabetes for at least 8 years and have varying degrees of coronary artery diseases. The mean CBMN frequency of the patients was statistically higher than that of the healthy control subjects (P < 0.05). The CBMN frequency was found to be significantly altered in CAN patients who where physical inactivity and smoking. A significant correlation could also be observed between CAN and smoking, diabetes mellitus, hypertension, dyslipidemia, abdominal obesity, and physical activity.     

Exhaled breath condensate – from an analytical point of view

Over the past three decades, the goal of many researchers is analysis of exhaled breath condensate (EBC) as noninvasively obtained sample. A total quality in laboratory diagnostic processes in EBC analysis was investigated: pre-analytical (formation, collection, storage of EBC), analytical (sensitivity of applied methods, standardization) and post-analytical (interpretation of results) phases. EBC analysis is still used as a research tool. Limitations referred to pre-analytical, analytical, and post-analytical phases of EBC analysis are numerous, e.g. low concentrations of EBC constituents, single-analyte methods lack in sensitivity, and multi-analyte has not been fully explored, and reference values are not established. When all, pre-analytical, analytical and post-analytical requirements are met, EBC biomarkers as well as biomarker patterns can be selected and EBC analysis can hopefully be used in clinical practice, in both, the diagnosis and in the longitudinal follow-up of patients, resulting in better outcome of disease.