薮北茶的组织培养

用薮北茶带腋芽的茎段作为外植体进行组织培养获得再生植株,并建立快速无性繁殖系.以MS为基本培养基,附加各种浓度的植物激素.试验结果表明,芽增殖培养基为1/2MS 1.0mg/L BA 0.2mg/L IBA;生根壮苗培养基为1/4MS 0.5mg/L BA 0.1mg/L IBA 0.01mg/L Al3 .     

栽培因子对白肋烟叶片组织结构的调控

研究表明：叶厚、栅厚、海厚、组织比、上表皮厚度等与烟叶品质和产量关系较密切的关键特征基本上都是TN86〉KY907〉TN90〉KY908〉K26〉KY8959，因而应以TN86，KY907，TN90的叶片组织结构较好．叶厚、栅厚、海厚、组织比、栅厚／叶厚及上下表皮厚度与栅栏组织细胞宽度等均随种植密度增加、封顶期后移和留叶数增多而呈下降趋势，但从低水平至中等水平的降幅很小，而从中水平至高水平的降幅较大；随施N量加大而呈上升趋势，低N至中N的升幅远大于中N至高N的升幅，施N量的作用大于种植密度，封顶期的效应远大于留叶数，且各项观测值均随叶位升高而增加．因而以18000株／hm^2，施N300kg／hm^2，全田50％现蕾后5d一次性封顶和留叶21～22片的试验组合的叶片组织结构较理想．叶片组织结构的关键特征均以完熟时最大、过熟时最小、初熟时在二者之间，下、中、上各叶位分别在封顶后第三、四、五周达完熟状态，兼顾各叶位宜在第4周砍收．     

植物组织培养中外植体发生褐变的原因及防止措施

通过对植物组织培养中外植体发生褐变的情况,分析了发生褐变的可能原因,并提出了相应的防止措施.     

药用、食用、观赏用百合组培快繁研究进展

百合是商品价值很高的植物品种,有药用、食用、观赏等用途．应用植物种苗组织培养快速繁殖技术,可在短期内生产大量优质的百合种苗．现阶段国内广泛开展了对百合的组织培养和植株再生途径的研究,百合的组织培养研究已较为成熟,多种外植体都可诱导分化出植株．本文从外植体和基本培养基的选择、激素对初代、增殖及生根培养的影响、组培苗移栽等方面,综述了近年来国内的药用、食用、观赏用百合组培快繁研究的现状．     

大花重瓣矮牵牛植株再生条件的研究

矮牵牛在生产中常采用播种繁殖。但是种子大都是从国外进口的,价格昂贵,为了更快的获得大量种苗,通过对矮牵牛进行了快速繁殖方法的实验．主要采用组织培养技术繁殖．经半年多的研究和配方试验,掌握了矮牵牛的组织培养配方及技术,大大缩短了其繁殖周期．并保持其品种的稳定性．     

金属烤瓷冠修复牙周组织的临床观察和研究

目的通过对临床病例的分析,探讨金属烤瓷冠修复对牙周组织的影响。方法选择全冠修复后因牙周肿胀不适就诊的61例患者共90颗患牙作为观察对象,检查牙周组织健康状况并进行临床评价。结果出现龈缘红肿的占37%,牙龈萎缩的占24%,邻间隙黑洞的占16%,牙石附着占23%,其中两种以上损伤占18%。结论金属烤瓷修复体的种类、形态、颈缘位置及密合度、光滑度和口腔卫生习惯均对牙周组织具有明显的影响。     

A stage-specific cell-manipulation platform for inducing endothelialization on demand

Endothelialization is of great significance for vascular remodeling, as well as for the success of implanted vascular grafts/stents in cardiovascular disease treatment. However, desirable endothelialization on synthetic biomaterials remains greatly challenging owing to extreme difficulty in offering dynamic guidance on endothelial cell (EC) functions resembling the native extracellular matrix-mediated effects. Here, we demonstrate a bilayer platform with near-infrared-triggered transformable topographies, which can alter the geometries and functions of human ECs by tunable topographical cues in a remote-controlled manner, yet cause no damage to the cell viability. The migration and the adhesion/spreading of human ECs are respectively promoted by the temporary anisotropic and permanent isotropic topographies of the platform in turn, which appropriately meet the requirements of stage-specific EC manipulation for endothelialization. In addition to the potential of promoting the development of a new generation of vascular grafts/stents enabling rapid endothelialization, this stage-specific cell-manipulation platform also holds promise in various biomedical fields, since the needs for stepwise control over different cell functions are common in wound healing and various tissue-regeneration processes.     

Injectable hybrid inorganic nanoscaffold as rapid stem cell assembly template for cartilage repair

Cartilage injuries are often devastating and most cannot be cured because of the intrinsically low regenerative capacity of cartilage tissues. Although stem-cell therapy has shown enormous potential for cartilage repair, the therapeutic outcome has been restricted by low survival rates and poor chondrocyte differentiation in vivo. Here, we report an injectable hybrid inorganic (IHI) nanoscaffold that facilitates fast assembly, enhances survival and regulates chondrogenic differentiation of stem cells. IHI nanoscaffolds that strongly bind to extracellular matrix (ECM) proteins assemble stem cells through synergistic 3D cell–cell and cell–matrix interactions, creating a favorable physical microenvironment for stem-cell survival and differentiation in vitro and in vivo. Additionally, chondrogenic factors can be loaded into nanoscaffolds with a high capacity, which allows deep, homogenous drug delivery into assembled 3D stem-cell-derived tissues for effective control over the soluble microenvironment of stem cells. The developed IHI nanoscaffolds that assemble with stem cells are injectable. They also scavenge reactive oxygen species and timely biodegrade for proper integration into injured cartilage tissues. Implantation of stem-cell-assembled IHI nanoscaffolds into injured cartilage results in accelerated tissue regeneration and functional recovery. By establishing our IHI nanoscaffold-templated 3D stem-cell assembly method, we provide a promising approach to better overcoming the inhibitory microenvironment associated with cartilage injuries and to advance current stem-cell-based tissue engineering.     

藏成药七十味珍珠丸中矿物质元素在体内蓄积性水平研究

名贵藏成药七十味珍珠丸是传统藏药的典型代表,疗效显著.其中矿物类药的大量运用为现代医学科学不可理喻,给传统藏医药学的健康发展带来了困惑。以金诃七十味珍珠丸为研究材料,以Wistar大白鼠为试验对象,用等离子体发射光谱(ICP AES)和氢化物原子吸收光谱(HAAS)等分析仪器,测定了对照组和给药组实验动物在连续 1 8周试验期间心、肝、肾等组织器官中的矿物质元素含量水平,阐述金诃七十味珍珠丸中矿物质元素在动物主要组织器官中的长期蓄积性及其对生物机体的毒性效应     

中药王不留行的组织培养技术初探

选取王不留行饱满种子培养无菌苗,用无菌苗的茎和叶作为外植体,分别接种MS基本培养基外加激素NAA和6-BA诱导愈伤组织和不定芽,使用1/2MS培养基,附加IBA或NAA进行不定芽的生根实验,最后炼苗和移栽.实验结果表明王不留行的最优组织培养方案:种子用0.1%升汞消毒8m in,无菌湿脱脂棉催芽,茎和叶作为外植体,使用MS 0.1mg/L NAA 0.1mg/L 6-BA诱导愈伤组织和不定芽,1/2MS 0.15mg/L NAA诱导植株生根.