芦荟的组织培养和快速繁殖

探索了芦荟组培快繁的最佳培养条件，使其试管内增殖速度达到了8－10倍；增殖周期缩短了20－25d，较常规组培的增殖速度3－5倍，增殖周期30－35d有了大幅度提高。     

爆仗竹组织培养快繁技术研究

以爆仗竹顶芽和带腋芽的茎段为外植体,研究不同激素组合及其浓度对爆仗竹组织培养的影响。结果表明:不同激素组合对爆仗竹芽的萌动、分化及生根培养有明显的影响,其中MS 6-BA2．0mg／L NAA0．8mg／L最适宜外植体芽的萌动;MS 6-BA1．0mg／L NAA0．5mg／L对丛生芽的继代增殖效果最好;MS NAA0．6mg／L对生根诱导效果最佳,生根率达100％。     

紫草试管快速繁殖

紫草(LithosPermum erythrorhizon)进行组织培养形成试管苗,应用LS基本培养基。用试管苗茎尖作外植体诱导愈伤组织即,使培养基的激素量减少到10％,愈伤组织诱导率仍比原植物作外植体高50％。当培养基添加05mg/l激动素和2mg/16苄基嘌呤,试管苗迅速增殖。当培养基无机盐减少一半,添加1mg/l的激素Ⅰ,接种物低温处理一定天数,根诱导率达80％至90％。     

仙人掌科植物组织培养研究进展

仙人掌科植物是一类日益受到重视的观赏、食用、药用植物。其组织培养研究在组培对象、外植体及其消毒、培养基、成苗途径等方面已取得了巨大进展,并对与植物学有关的研究领域产生了较大影响。     

Preparation and in vitro studies of microencapsulated cells releasing human tissue inhibitor of metalloproteinase-2

Objective: To prepare microencapsulated cells releasing human tissue inhibitor of metalloproteinase-2 (TIMP-2), and investigate their biological characteristics in vitro. Methods: Chinese hamster ovary (CHO) cells were stably transfected with a human TIMP-2 expression vector, encapsulated in barium alginate microcapsules and cultured in vitro. Morphological appearance of the microcapsules was observed under a light microscope. Cell viability was assessed using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Enzyme linked immunosorbent assay (ELISA) and reverse zymography were used to confirm the release of biologically active TIMP-2 from the microcapsules. Cryopreservation study of the microencapsulated cells was carried out using dimethyl sulfoxide (DMSO) as preservative agent. Results: The microcapsules appeared like a sphere with diameter of 300–600 μm. The surface of the capsule wall was clearly smooth. The microencapsulated cells survived well and kept proliferating over the 6 weeks observed. No significant difference in TIMP-2 secretion was found between encapsulated and unencapsulated cells. Reverse zymography confirmed the bioactivity of MMP (matrix metalloproteinase) inhibition of TIMP-2. The cryopreservation process did not damage the microcapsule morphology nor the viability of the cells inside. Conclusion: Microencapsulated engineered CHO cells survive at least 6 weeks after preparation in vitro, and secrete bioactive TIMP-2 freely from the microcapsules.     

黄鼬呼吸器官的组织学研究

本项研究结果表明;黄鼬呼吸器官的组织结构与其他哺乳动物呼吸器官的组织结构相比有其重要特征.(1)气管粘膜上皮内无杯状细胞;(2)粘膜下层内气管腺特别发达;(3)气管的外膜由1一4层弧形的透明软骨间断排列构成支架,软骨外侧平滑肌特别发达呈环行的带状;(4)肺内各级支气管壁平滑肌都较发达,粘膜皱襞明显;(5)肺泡隔厚,其结缔组织中的细胞成份多,排列紧密.     

生物组织光学特性参数测量原理及设计

从光的传输理论出发，利用漫射近似方程，推导出当无限细脉冲光及连续光束垂直入射到生物组织表面时，生物组织表面漫反射光随空间及时间变化的解析表达式，由此表达式出发，设计了一套实验装置，分析了生物组织光学特性参数的测量方法，以及如何对实验数据进行处理。     

果树组织培养脱毒技术和病毒检测技术研究进展

病毒病对果树的危害日趋严重,各国对病毒病害的研究和防治极为重视.作者着重论述当前果树生产上主要应用的果树组织培养技术、脱除病毒技术和病毒检测技术,包括其原理、操作过程和特点,并简要探讨了病毒检测与脱毒的关系.     

MEASUREMENT OF THE DIELECTRIC PROPERTIES OF BIOLOGICAL TISSUE

An open-ended coaxial line reflection method especially suitable for meas-uring the dielectric properties of biological tissue in vivo is described.This method offersthe advantage of not requiring any special preparation of the samples to be measured but aclose contact with the open end of a coaxial line.It is,therefore,very convenient to acquirea large number of measurement data in broad band rapidly.The method may also be usedto measure the properties of other substances.The measuring system consists of a networkanalyzer controlled by a microcomputer and calibrated by using ANA procedure to elimi-hate the influnce of error network introduced by the adapter,some connectors,etc.In or-der to reach higher accuracy,the iterative method is used to determine the parameters ofthe equivalent circuit.Measurements of permeativities of some living tissues have been per-formed in the frequency band of 0.5-2GHz.Compared with the results known in somepapers,the validity of this method has been confirmed.The difference in dielectric proper-ties between living and dead tissues,and the tissue permeativites(ε)versus frequency andduration of measurement after death have also been measured.     

祁术的组织培养及快速繁殖技术研究

为研究祁术的组织培养及快速繁殖技术,选用祁术的叶片和下胚轴为外植体材料,接种于附加植物生长调节剂NAA,6-BA及其组合的MS固体培养基上.结果表明:下胚轴为外植体明显好于叶片,对于愈伤组织形成,叶片采用MS NAA(0.5mg/L) 6-BA(2mg/L);下胚轴MS NAA(0.5mg/L) 6-BA(2mg/L);MS NAA(0.5mg/L) 6-BA(1mg/L)为宜.对于叶片丛生芽愈伤组织培养基用MS NAA(0.2mg/L) 6-BA(2.8mg/L),下胚轴丛生芽愈伤组织培养基用MS NAA(0.01mg/L) 6-BA(0.1mg/L);MS NAA(0.05mg/L) 6-BA(0.1mg/L)为宜,诱导生根以1/2MS NAA0.3mg/L最佳,生根率达95%以上.