Mass spectrometry-based protein-protein interaction techniques and their applications in studies of DNA damage repair

Proteins are major functional units that are tightly connected to form complex and dynamic networks.These networks enable cells and organisms to operate properly and respond efficiently to environmental cues.Over the past decades,many biochemical methods have been developed to search for protein-binding partners in order to understand how protein networks are constructed and connected.At the same time,rapid development in proteomics and mass spectrometry(MS)techniques makes it possible to identify interacting proteins and build comprehensive protein-protein interaction networks.The resulting interactomes and networks have proven informative in the investigation of biological functions,such as in the field of DNA damage repair.In recent years,a number of proteins involved in DNA damage response and DNA repair pathways have been uncovered with MS-based protein-protein interaction studies.As the technologies for enriching associated proteins and MS become more sophisticated,the studies of protein-protein interactions are entering a new era.In this review,we summarize the strategies and recent developments for exploring protein-protein interaction.In addition,we discuss the application of these tools in the investigation of protein-protein interaction networks involved in DNA damage response and DNA repair.     

DNA’s new avatar as nanoscale construction material

DNA is fast taking on a new aspect utilizing it’s physical property of persistence length and chemical property of base pairing to build architectures in 1D, 2D and 3D. This field is called structural DNA nano-technology and is poised to revolutionize several areas ranging from materials science to cell biology.     

灿烂甲酚蓝标记分光光度法测定核酸

研究了灿烂甲酚蓝与小牛胸腺DNA相互作用，提出了测定ctDNA的新的光度分析法。室温、pH8．1tris-HCl的条件下，ctDNA的加入使灿烂甲酚蓝在其最大吸收波长636nm处的吸光度明显下降，下降的程度与ctDNA的含量呈线性关系，ctDNA质量浓度在0～3．0μg／ml范围内与△A呈线性关系，线性回归方程为△A=0．0032 0．0984C(μg/ml）(r=0．9987)，方法的检出限（3σ／k)为0．125μg/ml。该方法具有仪器简单，快速，选择性好等特点，对合成样品中ctDNA测定，RSD％=1．5～1．9，回收率在101％～104％，结果令人满意。     

Effects of medium composition on the production of plasmid DNA vector potentially for human gene therapy

INTRODUCTION The difficulties associated with large-scaleproduction of biotherapeutics provide a constantchallenge to the biotechnology industry. FDA hadadded “therapeutic DNA plasmid vectors” to the listof well-characterized biotechnology product (DoHHs,1996), and gene therapy has moved rapidly fromlaboratory scale to clinical trials. It is urgent to de-velop new protocols to obtain high-quality plasmidswith high yields and minimal or no contamination ofRNA and chromosomal D…     

<生物的遗传>一节的教学后记

遗传学基础知识是高中生物课的重要内容,教好它对培养学生献身生命科学精神有重要意义.     

小量提取质粒DNA的简易方法

碱裂解法是目前最为广泛应用的质粒DNA的提取方法,本人利用0.9%NaC l溶液代替常规方法中的溶液Ⅰ,并缩短了一些步骤的反应时间,既达到了快速、简便提取质粒DNA的目的,又可以满足分子生物学后续实验的要求.     

核酸人工合成的研究进展

本文概述了人工合成核酸的最新研究进展     

春小麦外源DNA两次导入和导入后杂交及其后代醇溶蛋白电泳分析

本文采用A－PAGE方法,对外源DNA导入两次的四个后代变异系、一个后代变异株系的六个单株和导入后代间杂交的四个后代变异系进行了醇溶蛋白电泳分析。结果表明：1．外源DNA两次导入进一步强化了变异;2．A－PAGE方法不仅可用于验证外源DNA导入的变异后代,而且可检测后代变异系的遗传稳定性;3．使外源DNA导入后的变异后代间再进行杂交,能够丰富变异类型。而且导入技术与杂交方法的结合,可以互相补充,对作物育种具有重要意义。     

分子生物学领域常用限制性内切酶和DNA聚合酶外文字符的规范编排

工具酶是基因重组技术不可缺少的工具,已被广泛应用于DNA分子的克隆和序列分析,而其中最常用、在期刊中出现频率最高的就是限制性内切酶和DNA聚合酶(尤其是Taq酶);然而,在分子生物学技术领域中,对这些工具酶的编排,国家标准没有明确规定,书刊的处理也就自成一体,编排方式很混乱.