A novel way of liver preservation improves rat liver viability upon reperfusion

Background/aim： Currently, the liver is cold-preserved at 0-4 ℃ for experimental and clinical purposes. Here, we investigated whether milder hypothermia during the initial phase of the preservation period was beneficial for liver viability upon reperfusion. Methods： In the first set of experiments, rat livers were preserved either conventionally in clinically used histidine-trypthopan-ketoglutarate （HTK） solution （Group A： 45 min and Group B： 24 h） or by slow cooling HTK solution （from 13 ℃ to 3 ℃） during the initial 45 min of preservation （Group C： 24 h）. In the second set of experiments, additional groups of livers were evaluated： Group BB-preservation according to Group B and Group CC-preservation according to Group C. Further, some livers were preserved at 13 ℃ for 24 h. Livers were then reperfused using a blood-free perfusion model. Results： Bile production was approximately 2-fold greater in Group C compared to Group B. Alanine transaminase （ALT） and aspartate transaminase （AST） release into perfusate were 2-3-fold higher in Group B compared to Group C. No significant differences were found in ALT and AST release between Group C and Group A. Livers in Group CC compared to Group BB exhibited significantly lower portal resistance, greater oxygen consumption and bromosulfophthalein excretion into bile and lower lactate dehydrogenase （LDH） release into perfusate. Histological evaluation of tissue sections in Group BB showed parenchymal dystrophy of hepatocytes, while dystrophy ofhepatocytes was absent in Group CC. Livers preserved at 13 ℃ for 24 h exhibited severe ischemic injury Conclusion： These results suggest that the conventional way of liver preservation is not suitable at least for rat livers and that slow cooling of HTK solution during the initial phase of cold storage can improve liver viability during reperfusion.     

组胺对人肝星状细胞合成Ⅰ型胶原的影响

研究组胺对人肝星状细胞(HSC-T6)合成Ⅰ型胶原的影响,探讨肥大细胞(MC)在慢性肝疾病中的作用机理。采用不同浓度(5×10-6、10×10-6、50×10-6、100×10-6mol/L)组胺刺激体外培养的HSC-T6;并设立不同时间点(24、36、48h)分别收集培养上清液,ELISA法测定培养上清液中的Ⅰ型胶原含量。结果表明,组胺能明显促进HSC合成分泌Ⅰ型胶原,且存在一定的时效关系和量效关系,MC可以通过其分泌的生物活性介质(组胺)增强HSC-T6分泌Ⅰ型胶原,而参与慢性肝病发生发展过程。     

雅芳行贿中国官员被曝光公司业绩再巨亏

雅芳行贿事件再发酵。据外媒报道,雅芳涉嫌行贿中国官员和第三方咨询机构数十万美元的可疑资金,以获取中国直销牌照。雅芳财报显示2011年业绩巨亏。     

中药联合人工肝血浆置换治疗肝衰竭疗效观察

结合实际,针对中药联合人工肝血浆置换治疗肝衰竭的方法进行论述。     

安全用好中成药

我们身边有这样的人,一感冒就喝板蓝根,一便秘就吃牛黄解毒片,女性经期不适就吃乌鸡白凤丸……这些耳熟能详的中成药,是许多人眼里不折不扣的"万金油"。可是,与民众对中成药的依赖成反比的是,近年来因中成药使用不当带来的安全问题越来越多,应当按医嘱适量服用,否则对健康有危害。     

夏秋换季暖胃护胃是关键

胃痛为胃病临床常见的一种病证,中医学常常以此命为病名,相当于现代医学之急、慢性胃炎,胃溃疡,胃神经官能症等,均可出现上腹部疼痛,兼有脘腹胀闷,甚则见胀痛攻窜、泛酸烧心,多与情绪恼怒、焦虑、抑郁有关,中医称之为肝胃不和或肝气犯胃。     

初春养肝正当时——春季护肝养生全攻略

春季,人体的生理变化主要是:一是气血活动加强,新陈代谢开始旺盛;二是肝主藏血、肝主疏泄的功能逐渐加强,人的精神活动也开始变得活跃起来。所以,立春后,养生就得养肝。为何肝是疏泄的,还得养肝呢?中医师解释说,古人有句话,"满则损,缺则盈",正如同     

甘胆口服液对副溶血性弧菌引起的对虾肝胰腺损伤坏死病防治效果

副溶血弧菌引起的对虾急性肝胰腺坏死病是南美白对虾养殖危害最大的疾病之一,但目前缺乏有效的绿色防控手段。为探究甘胆口服液对南美白对虾急性肝胰腺坏死病的防治作用,在副溶血性弧菌人工感染对虾4 h后,通过投喂不同剂量（0.5、1.0、2.0、3.0、4.0、5.0 mL/kg饲料）的甘胆口服液进行治疗,连续用药1周后观察对虾肝胰腺病理损伤情况,测定血淋巴中丙氨酸氨基转移酶（ALT）与天门冬氨酸氨基转移酶（AST）含量,统计对虾发病率和感染死亡率。结果表明：甘胆口服液以2.0～5.0 mL/kg饲料的剂量给药7 d,可改善副溶血性弧菌感染引起的对虾肝胰腺细胞受损情况,有效抑制肝胰腺损伤导致的AST、ALT含量的异常升高,并将感染对虾死亡率降低至12%以下。甘胆口服液对副溶血性弧菌引起的对虾急性肝胰腺坏死病具有较好的治疗效果。