Planning Improves Vocal Fluency and the Appearance of Concern When Communicating Emotional Support

Emotional support is often conveyed to people with cancer; however, not all support messages are effective, leading some potential supporters to fear appearing incompetent when communicating support. Additionally, nonverbal behaviors, such as vocal fluency, pitch variety, eye contact, and conveying concern, have previously been associated with support recipients’ outcomes and perceptions of speaker competence. This experiment determines whether these nonverbal behaviors can be increased through message planning. Participants were randomly assigned to either a planning condition or a distraction task condition before recording emotional support messages for a friend hypothetically diagnosed with cancer. Results showed that planners spoke with significantly more vocal fluency and conveyed significantly more nonverbal concern than nonplanners. Planners also used more eye contact and pitch variety than nonplanners, but these differences were not statistically significant. Results suggest that planning may improve some nonverbal aspects of communicating support, which may in turn improve perceptions of supporters’ competence.     

高职院校学生社会化媒体信息甄别能力调查分析与启示

社会化媒体日益成为大学生信息获取的主要来源。文章通过文献分析法、问卷调查法,分析了高职院校学生社会化媒体信息甄别能力现状,提出了从强化信息质疑意识、培养信息求证精神、修练信息甄别技能和形成信息甄别氛围四个方面提升高职院校学生社会化媒体信息甄别能力,为高职院校阅读推广活动的开展与研究提供了广域的视野。     

悬臂筛网振动筛在本钢原料场的应用

研究XBSFJ型悬臂筛网振动筛的基本参数和工作原理,分析XBSFJ型悬臂筛网振动筛的结构特点。该振动筛在本钢原料厂的实际应用,证明了该振动筛的有效性,而通过与椭圆筛的对比分析,说明该悬臂筛具有更好的性能。     

Proteomic analysis of primary colon cancer-associated fibroblasts using the SELDI-ProteinChip platform

Objective: Cancer-associated fibroblasts (CAFs) are one of the hallmarks of the cancer microenvironment. Recent evidence has indicated that CAFs are more competent in enhancing cancer cell growth and migration than normal fibroblasts. However, the unique protein expression of CAFs has not been fully elucidated. This study aims to investigate the characterizations of colon CAFs by comparing the differential protein expression between CAFs and normal fibroblasts. Methods: Primary fibroblasts were isolated from surgical specimen of human colon cancer and matched normal colonic tissue. Purity of the cell population was verified through immunostain analysis. Total cell lysates and conditioned media from each group of cells were extracted, and protein expression analysis was conducted using the surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) ProteinChip platform. Results: Most primary cells showed typical fibroblast-like features after two weeks. Increased proportion of α-smooth muscle actin-positive myofibroblasts was detected within the CAFs in four of the six pairs of primary cells. Fibroblast activation protein was weakly expressed in most cells without differences. Using SELDI-TOF-MS ProteinChip platform, four protein peaks mass over charge ratio (m/z) 1142, 3011, 4035, and 4945 were detected in the total cell lysates, and two protein peaks m/z 1368 and 1389 were detected in the conditioned media. The potential candidate proteins found in the Swiss-Prot database include morphogenetic neuropeptides, FMRFamide-related peptides, insulin-like growth factor II, thymosin β-4-like protein 3, and tight junction-associated protein 1. Conclusions: Using the SELDI-ProteinChip platform, differential protein expressions were identified in colon CAFs compared with normal colonic stromal fibroblasts. The complex proteomic alternations in colon CAFs may play important roles related to the colon cancer microenvironment.     

废弃菌包中纤维素分解菌的分离与筛选

从废弃菌包中分离得到的11株菌株通过固体培养初筛和摇瓶发酵复筛,筛选出纤维素酶活性较高的2株菌株,分别记为f-6和f-10菌株。f-6菌株的Cx酶活力最高,作用于可溶性纤维素的效率最高;f-10菌株的复合酶活力最高,作用于不溶性纤维素的效率最高。在实际应用中,作用于可溶性的纤维素应该考虑选用菌株f-6,作用于不溶性的纤维素则考虑选用菌株f-10。     

瑞香狼毒提取物作用于人肺癌细胞NCI-H446[H446]后端粒酶的定量表达

目的:通过端粒酶的数量变化来探讨瑞香狼毒提取物对肺癌细胞NCI-H446[H446]的抗肿瘤作用。方法:体外培养肺癌细胞NCI-H446[H446]细胞株,以不同浓度的瑞香狼毒提取物作用24h后,用荧光标记的方法定量检测端粒酶。结果:瑞香狼毒提取物作用人肺癌细胞提取物NCI-H446[H446]后,端粒酶的数量明显减少并呈现与剂量的正相关性。结论:瑞香狼毒提取物对肺癌细胞NCI-H446[H446]具有明显的抗肿瘤作用。     

Community Childcare Providers’ Role in the Early Detection of Autism Spectrum Disorders

The first few years of life represent a crucial period for optimal brain development for young children. Therefore, it is important to identify children at-risk for developmental delays, including autism spectrum disorders, at the earliest age possible. An argument for utilizing community childcare providers for universal developmental screening is presented along with suggestions for assessment tool selection and a model for developmental monitoring in community childcare settings.     

浅析乳腺癌的早期诊断研究

乳腺癌是全球范围内严重威胁女性健康的主要恶性肿瘤,临床就诊患者的年龄普遍提前.如何有效的控制其发生和发展,做到早期发现,早期诊断,早期治疗,已经成为当务之急.乳腺自查,乳腺X线钼靶摄片,超声检查,MRI检查,CT检查,影像定位穿刺活检,乳头溢液检查都可提高乳腺癌的早期诊断率.     

细胞周期蛋白D1和细胞周期蛋白依赖激酶4在宫颈鳞癌中的表达

目的：探讨Cyclin D1和CDK4在正常宫颈上皮、CIN和宫颈鳞癌中表达状况。方法：收集子宫颈活检及手术标本118例，其中正常鳞状上皮14例，CIN25例，宫颈鳞癌79例。采用SP免疫组织化学方法，检测Cyclin D1和CDK4蛋白的表达情况。结果：Cyclin D1和CDK4在正常宫颈上皮、CIN和宫颈鳞癌的中表达水平存在显著性差异（P〈0．05），在不同分化程度的宫颈鳞癌间无显著性差异（P〉0．05）。结论：在宫颈鳞癌发生过程中伴右Cyclin D1和CDK4表达水平的增高．检测其含量改变有助于宫颈鳞癌诊断。