早期宫颈癌耐药基因与临床预后关系回顾性研究

目的：探讨耐药基因p-糖蛋白（p-gp）、谷胱甘肽-S-转移酶（GST-Ⅱ）、拓扑异构酶Ⅱ（TOpoⅡ）、胸苷酸合成酶（TS）在宫颈癌中的表达及临床意义。方法：应用免疫组织化学方法检测65例宫颈癌中p-gp、TOpoⅡ、GST-π、TS的表达，并分析其与临床预后之间的关系。结果：p-gp的表达与肿瘤分化程度、淋巴结转移有关（P〈0．05），与UICC分期、局部复发无关（P〉0．05）；TOpoⅡ的表达与淋巴结转移、局部复发有关（P〈0．05），与肿瘤分化程度、UICC分期无关（P〉0．05）；GST-π的表达与各个指标之间均存在相关（P〈0．05）；TS与淋巴结转移、UICC分期有关（P〈0．05），与局部复发、肿瘤分化无关（P〉0．05）。经多因素COX比例风险模型分析显示：局部复发、淋巴结转移及TOPOⅡ、GST-π的表达与宫颈癌患者术后生存率有关（P〈0．05）。结论：宫颈癌中p-gp、TOpoⅡ、GST-π、TS的联合检测对判断宫颈癌的预后十分必要；局部复发、淋巴结转移及TOpoⅡ、GST-π的表达可作为宫颈癌患者独立的预后因素。     

Usefulness of serum CA 15.3 and histopathological prognostic indices in breast cancer

Prognostic factors influence the modality and course of therapy in breast cancer. These include both histopathological and biochemical variables. This study was aimed to find out if any correlation exists between the biochemical tumor marker-serum CA 15.3, and the lymph node staging of Nottingham’s Prognostic Index (NPI). It was observed that serum CA 15.3 showed a statistically significant correlation with lymph node staging of NPI. Therefore it is concluded that CA 15.3 can be utilized as a useful prognostic marker, particularly in cases where histopathological variables such as tumor size and grade have been altered by pre-operative chemotherapy.     

Examining the predictive validity of behavior screeners across measures and respondents

Through this study we compared different informants and different behavior screening tools that are available to screen for behavioral and emotional risk. We examined screening results from 100 students from a high school with a high achiever's magnet program in the Southeastern United States (school demographics: 71% female and 79% black, non-Hispanic). This school conducted behavior screening using both teacher-report via the Student Risk Screening Scale, Internalizing/Externalizing (SRSS-IE) and student report via the Strengths and Difficulties Questionnaire (SDQ). Nonparametric correlation tests were conducted between the SRSS-IE and SDQ externalizing and internalizing scores. Generalized linear regression models were created based on the data (demographics, internalizing, and externalizing scores on both screening tools) to model the two count outcomes (office discipline referrals [ODRs] and absences). Diagnostic accuracy metrics were generated from the scores on both the SRSS-IE and SDQ with the outcome measures (ODRs and absences). Externalizing scores on both screening tools had statistically significant low correlations. The ODR model contained two predictors: externalizing score on the SRSS-IE and internalizing score on the SDQ, while the absence model contained grade and the externalizing score on the SDQ. The highest accuracy and agreement values were seen between students with elevated risks on both screening measures and ODRs. The results confirmed that teacher and student reporting, as well as different screening tools, will result in some different students being identified. The choice of informant and screening tools should be dependent on the needs and resources of the school.     

Injury prevention in male youth soccer: Current practices and perceptions of practitioners working at elite English academies

Forty-one practitioners inclusive of physiotherapists, sports scientists and strength and conditioning coaches from the academies of elite soccer clubs in the United Kingdom completed an on-line questionnaire which examined their: (1) background information; (2) perceptions of injury occurrence and risk factors; (3) screening and return to play; and (4) approach to designing and delivering injury prevention programmes with a response rate of 55% (41/75). Contact injuries were the most common mechanism reported and players between 13–16 years of age were perceived to be at the greatest risk. Pertinent risk factors included: reduced lower limb and eccentric hamstring strength, proprioception, muscle imbalances, and under developed foundational movement skills. Joint range of motion, jump tests, the functional movement screen, overhead and single leg squats were the most utilised screening methods. Training modalities rated in order of importance included: resistance training, flexibility development, agility, plyometrics and balance training. Training frequency was most commonly once or twice per week, during warm-ups, independent sessions or a combination of both. Injury prevention strategies in this cohort appear to be logical; however, the classification of injury occurrence and application of screening tools to identify “at risk” players do not align with existing research. The frequency and type of training used may also be insufficient to elicit an appropriate stimulus to address pertinent risk factors based on current recommendations.     

The one-week and six-month reliability and variability of three-dimensional tibial acceleration in runners*

Tibial acceleration is a surrogate measure for impact loading and lower limb fatigue injury in runners. Triaxial accelerometers may offer reliable and practical measurement of resultant peak tibial acceleration (PTA). With such potential in mind, this study examined variability and measurement reliability of tibial acceleration in 14 runners at baseline at one week, and eight of the runners again at six months. Triaxial accelerometers were attached to the distal tibiae of runners before they ran on a treadmill for two minutes each, at speeds of 2.7, 3.0, 3.3 and 3.7 m/s in standardised shoes. Resultant PTAs were calculated for each speed and session. Reliability outcomes were presented as percentage change and effect sizes. Variability outcomes included intraclass correlation coefficients and the typical error of the measurement. Smallest worthwhile change and performance/noise ratio were also calculated. While runners demonstrated marginally lower reliability and higher variability over six months, compared to one week, in all cases the measures of reliability and variability were of ‘good’ to ‘moderate’ reliability, and ‘small’ to ‘moderate’ variability using magnitude-based inferences. We can be confident that resultant PTA can be used with runners to assess and monitor their impacts throughout a six-month intervention.     

Evaluation of pelvic lymph node coverage of conventional radiotherapy fields based on bony landmarks in Chinese cervical cancer patients using CT simulation

ncer. CT simulation may be a feasible technique for planning pelvic fields optimally and individually.     

肿瘤细胞免疫治疗相关伦理学探讨（英文）

本文旨在对细胞免疫治疗相关的伦理学问题进行探讨,并通过分析我国细胞免疫治疗相关伦理学实践及现状,为规范我国肿瘤细胞免疫治疗的伦理标准提供参考。随着免疫学、分子生物学等学科的飞速发展和高通量测序等技术的普及,肿瘤免疫治疗已成为第四大肿瘤治疗方法。肿瘤细胞免疫治疗,如各种基于T细胞、自然杀伤(NK)细胞和树突状细胞(DC)的免疫治疗在其中发挥了重要作用。随着临床实践的开展,一些相关的伦理学问题逐渐暴露出来。如何在治疗过程中坚持基本伦理原则,有效指导并解决相关临床问题,提高治疗效果,保护受试者权益,是目前亟需研究的重要课题。本文对与之相关的伦理学问题进行探讨,并通过分析我国细胞免疫治疗相关伦理学实践及现状,以期为规范我国肿瘤细胞免疫治疗的伦理标准提供参考。     

β－葡萄糖苷酶产酶菌株发酵条件的优化研究

通过菌种筛选，确定了黑曲霉Ｎ５为β－葡萄糖苷酶的高产菌株。通过单因子及正交试验，对培养基优化后，酶活由２．９６μ／ｍｌ增加到９６．５μ／ｍｌ，提高了近３０倍，最终得到了β－葡萄糖苷酶发酵的最适条件．     

Uncertain Terms: Message Features of Online Cancer News

About 113 million Americans have reported seeking health information online; however, little is known about the quality (or qualities) of the information being retrieved. Users have reported seeking information about health issues in an attempt to reduce negatively appraised uncertainties (Brashers, 2007), but less is known about the ability of the retrieved information to increase or produce unwanted uncertainty. A content analysis of online cancer news was conducted and suggests that 65% of Internet-based cancer news contains message features previously linked to the production of uncertainty (e.g., ambiguous or complex information). Though future research is required regarding uncertainty-related content and its effects, this project provides a foundation for such future endeavors.     

Elevated skin autofluorescence is strongly associated with foot ulcers in patients with diabetes: a cross-sectional, observational study of Chinese subjects

Objective  

This study was designed to evaluate the association between skin autofluorescence (AF), an indicator of advanced glycation end-products (AGEs), and foot ulcers in subjects with diabetes.