刺五加注射液对 Hela 细胞抑制作用的实验研究

目的:探讨刺五加注射液对人宫颈癌细胞株Hela体外增殖的抑制作用。方法:采用MTT法测定了不同浓度的刺五加注射液对人宫颈癌细胞株Hela的体外抑制作用,倒置显微镜观察细胞形态变化。结果:刺五加注射液浓度越高对Hela细胞抑制作用越好,作用时间越长抑制效果越强,量效关系和时效关系良好;形态学观察到细胞凋亡。结论:刺五加注射液对人宫颈癌细胞株Hela的细胞增殖有较强的抑制作用。     

Differential erythrocyte agglutination pattern in normal and cancer patients with Synadenium grantii root (Hook f) lectin

In the present study the property of lectin agglutination in blood on normal and different cancer patients has been observed. The purifiedSynadenium grantii root lectin was non blood group specific and its utility as a diagnostic tool in malignancy was studied. Hemagglutination (units/ml) of red blood cells of different types of cancer was compared with the normal control's red blood cells. Out of 113 total cancer patients, only a group of 29 breast cancer patients showed significant increase in titre value (P<0.05) compared to normal control.     

Association of hypomethylation of LINE-1 repetitive element in blood leukocyte DNA with an increased risk of hepatocellular carcinoma

Global DNA hypomethylation has been associated with increased risk for cancers of the colorectum, bladder, breast, head and neck, and testicular germ cells. The aim of this study was to examine whether global hypomethylation in blood leukocyte DNA is associated with the risk of hepatocellular carcinoma (HCC). A total of 315 HCC cases and 356 age-, sex- and HBsAg status-matched controls were included. Global methylation in blood leukocyte DNA was estimated by analyzing long interspersed element-1 (LINE-1) repeats using bisulfite-polymerase chain reaction (PCR) and pyrosequencing. We observed that the median methylation level in HCC cases (percentage of 5-methylcytosine (5mC)=77.7%) was significantly lower than that in controls (79.5% 5mC) (P=0.004, Wilcoxon rank-sum test). The odds ratios (ORs) of HCC for individuals in the third, second, and first (lowest) quartiles of LINE-1 methylation were 1.1 (95% confidence interval (CI) 0.7–1.8), 1.4 (95% CI 0.8–2.2), and 2.6 (95% CI 1.7–4.1) (P for trend <0.001), respectively, compared to individuals in the fourth (highest) quartile. A 1.9-fold (95% CI 1.4–2.6) increased risk of HCC was observed among individuals with LINE-1 methylation below the median compared to individuals with higher (>median) LINE-1 methylation. Our results demonstrate for the first time that individuals with global hypomethylation measured in LINE-1 repeats in blood leukocyte DNA have an increased risk for HCC. Our data provide the evidence that global hypomethylation detected in the easily obtainable DNA source of blood leukocytes may help identify individuals at risk of HCC.     

辊筒四色印花工艺的研制

介绍了辊筒四色印花的工艺过程，包括图案设计、分色加网、花筒腐蚀以及印花等工艺环节、该工艺简单实用、可靠性强、精度高。     

Science Letters: Vascular architecture: is it a helpful histopathological biomarker for hepatocellular carcinoma?

Hepatocellular carcinoma (HCC) remains one of the major public health problems throughout the world. Although originally associated with tumorigenic processes, liver angiogenesis has also been observed in the context of different liver inflammatory, fibrotic, and ischemic conditions. Here we investigate the fractal dimension as a quantitator of non-Euclidean two-dimensional vascular geometry in a series of paired specimens of primary HCC and surrounding non-tumoral tissue, and discuss why this parameter might provide additional information regarding cancer behavior. The application of fractal geometry to the measurement of liver vascularity and the availability of a computer-aided quantitative method can eliminate errors in visual interpretation, and make it possible to obtain closer-to-reality numerals that are compulsory for any measurement process.     

Influence of CO2 pneumoperitoneum on intracellular pH and signal transduction in cancer cells

Object: The authors studied the influence of CO2 pneumoperitoneum on intracellular pH and signal transduction arising from cancer cell multiplication in laparoscopic tumor operation. Method: They set up a simulation of pneumoperitoneum under different CO2 pressure, and then measured the variation of intracellular pH (pHi) at different time and the activity of protein kinase C (PKC) and protein phosphatase 2a (PP2a) at the end of the pneumoperitoneum. After 1 week, the concentration of cancer cells in the culture medium was calculated. Result: When the pressure of CO2 pneumoperitoneum was 0, 10, 20, 30 mmHg respectively, the average pHi was 7.273, 7.075, 6.783, 6.693 at the end of the pneumoperitoneum; PKC activity was 159.4, 168.5, 178.0, 181.6 nmol/(g-min) and PP2a was 4158.3,4066.9, 3984.0, 3878.5 nmol/(g-min) respectively. After 1 week, the cancer cells concentration was 2.15×105, 2.03×105, 2.20×105, 2.18×105 L-1. Conclusion: CO2 pneumoperitoneum could promote acidosis in cancer cells, inducing the activation of protein kinase C and deactivation of protein phosphatase 2a, but it could not accelerate the mitosis rate of the cancer cells.     

Correlates of early reading performance in a transparent orthography

A cross-sectional study was conducted with 83first – and 81 fourth graders at the end of theschool year to examine factors accounting forearly reading performance in Finnish, atransparent orthography with a clear mapping ofphonemes onto graphemes. Measures for bothgrades included reading comprehension, phonemeawareness, and object- and digit naming.Additionally measures of skills in morphology,spelling and a screening battery wereadministered to the first graders. The sets ofmeasures accounted for 56% of the variance inreading performance in first grade and 64% infourth grade. Phoneme awareness was stronglyrelated to reading performance and spelling atthe end of first grade, but only forless-skilled readers in fourth grade. Theseresults suggest a larger role for phonemeawareness for children learning a transparentorthography than has been suggested in earlierstudies. At the same time, listeningcomprehension contributed more strongly tofirst-grade reading performance than has beenreported for children learning to readEnglish.     

卵巢上皮癌组织COX-2的表达及与MVD的关系

目的:探讨卵巢上皮癌组织中环氧合酶-2(COX-2)表达及其与微血管密度(MVD)的关系。方法:应用免疫组织化学技术检测65例卵巢上皮癌组织和18例正常卵巢组织中COX-2的表达,并以抗CD34抗体标记微血管内皮细胞测定MVD。结果:卵巢上皮癌组织COX-2表达阳性的比例为73.8%,显著高于正常卵巢组织COX-2表达阳性的比例11.1%(P<0.01)。COX-2阳性表达的卵巢上皮癌组织中的MVD(57.8±18.6)明显高于阴性表达者(37.4±11.2)(P<0.01)。结论:COX-2在卵巢上皮癌组织中高表达,COX-2可促进肿瘤血管生成。     

A study of women's compliance‐gaining behaviors in violent and non‐violent relationships

Recent research by communication scholars has investigated the dynamics of abusive spousal relationships (Chandler, 1986; Infante, Chandler, & Rudd, 1989; Infante, Chandler‐Sabourin, Rudd, & Shannon, 1990; Rancer & Niemasz, 1988; Rudd, Burant, & Beatty, 1994; Sabourin, Infante, & Rudd, 1993). Infante and his colleagues have suggested that those involved in violent relationships communicate differently with their partners than those involved in nonviolent relationships. Based on this prior research, it seems important for communication scholars to further investigate the communication behaviors of individuals involved in violent relationships. This current study seeks to advance the family violence research by comparing the types of compliance‐gaining strategies that battered and non‐battered women report using during their disputes.     

“Kind,Sensitive, and Above All,Honest”: Long-Term Cancer Survivors’ Quality of Life and Self-Advocacy

ABSTRACT

Long-term survivors of cancer (LTS) are a growing population whose needs differ significantly from patients undergoing cancer treatment. Many LTS suffer from late effects of cancer treatments or symptoms that persist after treatment concludes or appear following remission. This study explored the relationship between LTS perceptions of quality of life with late effects and perceptions of patient self-advocacy through quantitative and qualitative analyses. A negative statistical relationship emerged between the variables. LTS median self-advocacy scores were quite high, reflecting their preference for health-care providers who listen actively, understand patient needs, are knowledgeable and compassionate, and utilize joint decision making.