Enhanced production of thermostable laccases from a native strain of Pycnoporus sanguineus using central composite design

研究目的：优化获得血红密孔菌（P.sanguineus）的最佳培养基组成,提高耐热漆酶的产量。创新要点：获得了目前文献报道的最高水平的漆酶活力。研究方法：通过单因素试验研究了不同培养基（番茄汁、麦麸、麦芽提取物和葡萄糖细菌蛋白胨培养基）和不同组合诱导剂（大豆油、阿魏酸、没食子酸、二甲基苯胺、酸性蓝62和活性蓝19分别与硫酸铜组合诱导剂）对P.sanguineus产耐热漆酶的影响。在此基础上采用中心组合试验设计,进一步研究了番茄汁培养基结合硫酸铜和大豆油组合诱导剂对P.sanguineus产耐热漆酶的影响。利用SAS10.0和响应面分析方法对试验结果进行了统计分析和建立回归模型。重要结论：通过中心组合设计优化得出P.sanguineus产耐热漆酶的最优培养基条件：以36.8%番茄汁为培养基,以3 mmol/L硫酸铜和1%大豆油作为组合诱导剂。该条件下在10 L搅拌槽生物反应器中漆酶活力达到了143 000 IU/L（2,2＇-联氮双（3-乙基苯并噻唑啉-6-磺酸）为底物,pH值为3.0）。     

Copper and Zinc Status in Psoriasis: Correlation with Severity

Psoriasis is a common and an inflammatory skin disease. Trace elements play an important role in the skin metabolism such as keratinisation and melanin formation as well as immunological and inflammatory reactions. Henceforth, the present study was aimed to evaluate the essential metals with special reference to Cu and Zn. In this study, 72 psoriatic patients and 50 controls were enrolled. On the basis of PASI score psoriatic group comprising of 48 mild and 24 severe psoriatic patients were grouped. The serum trace elements analysis in the subjects revealed that serum Cu levels and Cu/Zn ratio was significantly higher in the psoriatic patients as compared to controls. Interestingly, severity of the psoriasis was well correlated with the serum Cu levels. Taking together, all these findings suggest that Cu may be a major culprit in the pathogenesis as well as in the severity of the disease.     

金刚石/铜复合材料传热数值模拟

本文采用有限元方法对金刚石/铜复合材料的传热性进行数值模拟,建立多颗粒二维随机分布复合材料测试模型,研究金刚石颗粒体积分数、颗粒半径、颗粒形貌、粒径比及界面因素对金刚石/铜复合材料传热性能的影响规律及机制。结果表明:金刚石/铜复合材料的热导率随着金刚石体积分数的增大而升高;热导率随着粒径的增大而呈现先升高后降低的规律;方形金刚石颗粒的复合材料热导率高于球形颗粒的复合材料;在金刚石体积分数一定时,适量配比细颗粒可提高材料热导率;对金刚石表面镀覆层新物质(W、Cr、Ti)来等效金刚石和铜二者物理结合时的界面热阻。     

沪铜期货套期保值有效性实证研究

铜期货已经成为铜行业上中下游企业不可或缺的定价和风险管理工具,越来越多铜企业参与到了铜期货套期保值中。然而期货价格与现货价格并非平行运动,即它们之间的价格变动不完全一致,因此,套期保值的关键在于套期保值比率的确定。本文从上海期交所选取了490个期货和现货数据进行探讨与研究,主要运用OLS模型、EMC模型对套期保值进行估算,并对估算结果进行了分析。     

铜胁迫对小叶黄杨生理生态特征的影响

采用水培试验的方法,研究不同浓度铜（0、20、40、60、80和100mg/L）胁迫条件下小叶黄杨的生长和生理特征。结果表明,随铜浓度的增加,小叶黄杨的生物量（鲜重）逐渐下降;蛋白质含量呈下降趋势;叶绿素含量先上升后下降;丙二醛的含量逐渐增加;超氧化物歧化酶和过氧化物酶的活性均呈先增加后下降的趋势。     

火焰原子吸收光谱法测定五味子、榛蘑、蕨菜中的铜和锌

利用火焰原子吸收光谱法测定了五味子、榛蘑、蕨菜中的铜和锌含量。RSD为0.46％～1．2％，回收率为96．0％～100．4％，该方法简便，灵敏度高，结果准确，可用于中草药中微量元素的测定。     

Toxicology and free radicals scavenging property of Tamra bhasma

Free radicals are implicated in various chronic diseases. There has always been a search for new antioxidants. In this paper we have investigated Tamra bhasma, a metallic ayurvedic preparation. It is a time-tested medicine in Ayurveda and is in clinical use for various ailments specifically the free radical mediated diseases. Our results show that Tamra bhasma inhibits lipid peroxidation (LPO), prevents the rate of aerial oxidation of reduced glutathione (GSH) content and induces the activity of superoxide dismutase (SOD) in rat liver homogenate in the bi-phasic manner. The drug was orally given for 7, 15 and 30 days in different doses. Best protective response was found at the dose of 0.5mg/100g body weight in albino rats, although it showed some histopathological changes at the dose of 20mg/100g body weight. The results suggest that this Ayurvedic preparation is not merely a source of copper metal, but it is a strong anti-oxidant with no detectable adverse effect in lower doses of therapeutic range.     

壳聚糖预富集火焰原子吸收光谱法测定中草药中痕量铜

研究中草药中的微量元素是中医现代课题之一,广大科技工作者认为中草药对各类疾病有较好疗效的物质基础之一是其中的微量元素[1]·铜是人体必须的微量元素之一,铜元素对血液循环有重要作用,它能维持动脉管壁的弹性、保护内膜、防止脂质渗透、对神经系统具有重要调节作用[2]·基     

WO3电极光电化学保护金属铜

采用WO3薄膜电极对金属铜光电化学防腐蚀。考察了光反应体系组成对电极光电响应性能的影响。结果表明：在光电化学电池中，阳极室添加空穴捕获剂如甲酸和升高溶液pH，有利于光电流输出。在阳极室溶液组成为0．5mol·dm^-3Na5O4＋0．5mol·dm^-3HCOOH（pH9.6）时，WO3薄膜电极在光照下可对4mol·dm^-3NaCl溶液中金属Cu进行光电化学防腐蚀。     

日粮中添加铜对小鼠血清和肝脏中铜、锌、锰的影响

96只小白鼠,随机分为4组.日粮中铜的添加量分别为0、60、120、180mg/kg,进行为期30d的试验,观察饲料中添加铜对小鼠血清和肝脏中铜、锌、锰的影响.结果表明:小鼠肝脏、血清中铜水平随日粮中铜添加量的升高而明显升高(P＜0.05),而肝脏、血清中锌、锰含量明显下降(P＜0.05).铜与锌、锰吸收与代谢之间存在颉抗关系.