康脑液对皮质区干细胞因子基因表达的影响

目的:在康脑液方剂干预下观察皮质区内源性神经细胞干细胞因子(SCF)mRNA在大鼠脑缺血再灌注损伤后不同时间的表达情况。方法:成年SD大鼠,以线栓法建立大脑中动脉缺血再灌注模型,随机分为模型组、药物干预组和假手术组。原位杂交技术检测脑缺血1.5h再灌注1～14d后,脑皮质区SCFmRNA表达情况。结果:脑缺血再灌注后,药物干预组、模型组SCFmRNA的表达在皮质区均明显高于假手术组,于第7天达高峰,第14天下降。结论:药物干预后脑缺血再灌注脑皮质区SCFmRNA表达在不同时间点与模型组具备相同的表达规律,两组SCFmRNA的表达无显著性差异。     

Study on the expression and mutation of human telomeric repeat binding factor ( hTRF1 ) in 10 malignant hematopoietic cell lines

Objective: Detecting the expression and mutation of human telomeric repeat binding factor (hTRF1) in 10 malignant hematopoietic cell line cells on the base of determining its genomic structure and its four pseudogenes to clarify if hTRF1 mutation is one of the factors of the activation of telomerase. Methods: hTRF1cDNA sequences were obtained from GenBank, its genome structure and pseudogenes were forecasted by BLAST and other biology information programs and then testified by sequencing. Real-time RT-PCR was used to detect the expression of hTRF1mRNA in 10 cell line cells, including myelogenous leukemia cell lines K562, HL-60, U-937, NB4, THP-1, HEL and Dami; lymphoblastic leukemia cell lines 6T-CEM, Jurkat and Raji. Telomerase activities of cells were detected by using telomeric repeat amplification (TRAP)-ELISA protocol. PCR and sequencing were used to detect mutation of each exon of hTRF1 in 10 cell line cells. Results: hTRF1 gene, mapped to 8q13, was divided into 10 exons and spans 38.6 kb. Four processed pseudogenes of hTRF1 located on chromosome 13, 18, 21 and X respectively, was named as ψhTRF1-13, ψhTRF1-18, ψhTRF1-21 and ψhTRF1-X respectively. All cell line cells showed positive telomerase activity. The expression of hTRF1 was significantly lower in malignant hematopoietic cell lines cells (0.0338, 0.0108:_0.0749) than in normal mononuclear cells (0.0493, 0.0369:_0.128) (P=0.004). But no significant mutation was found in all exons of hTRF1 in 10 cell line cells. Four variants were found in part of intron 1, 2 and 8 of hTRF1. Their infection on gene function is unknown and needs further studies. Conclusion: hTRF1 mutation is probably not one of the main factors for telomerase activation in malignant hematopoietic disease. Project supported by the National Basic Research Program (973) of China (No. 2002CB713700) and the National Natural Science Foundation of China (No. 39870339)     

Transient expression of chicken alpha interferon gene in lettuce

We investigated the possibility of producing chicken alpha interferon (ChIFN-α) in transgenic plants.The cDNA encoding ChIFN-a was introduced into lettuce (Lactuca sativa L.) plants by using an agro-infiltration transient expression system.The ChIFN-α gene was correctly transcribed and translated in the lettuce plants according to RT-PCR and ELISA assays.Re-combinant protein exhibited antiviral activity in vitro by inhibition of vesicular stomatitis virus (VSV) replication on chicken embryonic fibroblast (CEF).The results demonstrate that biologically active avian cytokine with potential pharmaceutical ap-plications could be expressed in transgenic lettuce plants and that it is possible to generate interferon protein in forage plants for preventing infectious diseases of poultry.     

Family Communication Patterns as Predictors of Organizational Dissent: A Replication Study

This study replicated a previous research project addressing connections between family-of-origin communication and organizational dissent expression. We predicted that family communication patterns (FCPs), specifically conversation and conformity orientations, would predict upward, lateral, and displaced dissent. As in the original study, significant findings emerged for upward dissent and conformity orientation. This replication study indicated family type as an inconsistent predictor of expressed dissent. Contrary to the original study, we found no evidence to support a connection between pluralistic family types and upward dissent. Finally, as an extension of the original study we analyzed effects of FCPs on displaced dissent, finding an inverse association with conversation orientation. Implications for future research are discussed.     

“搞”的艺术化用法探略

阐述“搞”的艺术化用法及其修辞效果,澄清对“搞”字运用的某种误解。认为“搞”具有随意性、口语化、概括性等特点,实际运用中出现的适应特定语境之用法,含有亲切、谦虚、个性化等意味,能表达出各种复杂的意思,可以收到精炼简洁、轻松诙谐、容易理解等表达效果。有的带“搞”的说法还呈现凝固化、词汇化趋势,说明某些“搞”字在词语组合中具有不可替代的功用。     

大学生非理性行为探析

处于青年中期大学生 ,受生理和社会经济环境的影响 ,在社会交往中容易产生非理性行为。本文拟就大学生非理性行为的表现、原因及矫正大学生非理性行为的对策作一初浅探析 ,以引导大学生理智克服非理性行为     

试谈翻译中理解的误区

理解原作是一切翻译活动不可或缺的环节,是正确表达的基础和前提,也是一项很艰巨的工作.本文以<棋王>英译文例,就其在多义词、句子结构、文化词语三方面对原作一些句子的理解提出质疑,并给出参考译文.     

孔子忠恕思想浅析

孔子的“忠恕”是一个统一的概念,而非两个并列的概念。其表述为“己所不欲,勿施于人”,任何所谓“肯定表述”都非孔子原有之意。“忠恕”与“仁”、“圣”等概念有密切的联系,与它们构成一个完整的道德体系。“忠恕”的思想基础是建立在人的共同情感基础上的。     

形式语义学研究两题

形式语义学奠基人蒙太格在其语句系统中设置了两个初始类型：对应个体的类型e与对应真值的类型t。按照传统哲学的观点,专名指个体,但是在蒙太格的系统中,专名不直接解释成论域中的个体,它表示性质的集合,对应类型〈〈e,t〉,t〉。这样的类型匹配方式实现了专名与量化短语的统一处理,是蒙太格语法的独到之处,有着深刻的集合论基础。     

汉字形体变化影响形声字"声符"表意

形声字“声符”表意日益复杂化的另一促成因素,是形声字本身形体结构的变化“。声符”书写变式、变体“,声符”形体改换、借用“,声符”形近,声近混误,讹传的形声字“的声符”误认,或文字归并,致使形声字“的声符”异字同体,因而产生庞大“的共用同一声符”的形声字字群,从而搅乱了形声字“声符”表意的直接性,有序性,往往造“成声符”表意的隐晦,游离。