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101.
Feng-ying LI Xiao-bin REN En-ping XU Qiong HUANG Hong-qiang SHENG Bing-jian LV Mao-de LAI 《Journal of Zhejiang University. Science. B》2010,11(4):258-266
Regenerating gene IV (RegIV),a member of the regenerating gene family discovered in 2001,has been found to be involved in malignancy in several different organs including the stomach,colorectum,pancreas and prostate,but the overall expression profile of RegIV has not been reported. To learn more about RegIV,we evaluated its distribution by immunohistochemistry (IHC) in a total of 360 samples including 24 types of normal tissue,40 benign and malignant lesions,and 18 neuroendocrine tumors. We found that in no... 相似文献
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Sun HC Xue FM Qian K Fang HX Qiu HL Zhang XY Yin ZH 《Journal of Zhejiang University. Science. B》2006,7(4):324-330
To develop a gene therapy strategy for treating bovine mastitis, a new mammary-specific vector containing human lysozyme (hLYZ) cDNA and kanamycin resistance gene was constructed for intramammary expression and clinical studies. After one time acupuncture or intracisternal infusion of healthy cows with 400μg of the p215C3LYZ vector, over 2.0 μg/ml ofrhLYZ could be detected by enzymatic assay for about 3 weeks in the milk samples. Western blotting showed that rhLYZ secreted into milk samples from the vector-injected cows had molecular weight similar to that of the natural hLYZ in human colostrums. Twenty days after the primary injection, the quarters were re-injected with the same vector by quarter acupuncture and even higher concentrations of rhL YZ could be detected. Indirect competitive ELISA of milk samples showed that the vector injection did not induce detectable humoral immune response against hL YZ. Clinical studies showed that twice acupuncture of quarters with the p215C3LYZ vector had overt therapeutic effect on clinical and subclinical mastitis previously treated with antibiotics, including disappearance of clinical symptoms and relatively high microbiological cure rates. These data provide a solid rationale for using the vector to develop gene therapy for treating bovine mastitis. 相似文献
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作为唐代清乐主流的清商曲辞,其比重不如相和、杂曲;作为唐代清商曲主流的三十二调,其歌辞比重也不如清商曲辞中的其他曲调.这两"少"说明衰落了的音乐在一定程度上对歌辞的创作具有抑制作用.娱乐性的歌辞表达的往往是一种泛化的情感,这并不利于作者情感的抒发.娱乐性的减弱使唐代诗人得以借乐府古题来抒发其强烈的感情. 相似文献
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Maya是现在最为流行的顶级3D动画软件。本文通过对卡通人物脸部模型的构建和面部表情的制作原理研究,然后运用Maya建立人物卡通模型,并制作出卡通的表情。 相似文献
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Nonverbal communication is the primary channel for the communication of emotion. The abilities to express, control, and decode emotions are important social skills. In this study, we investigated the effects of hemispheric brain dominance and sex on emotional social skills, specifically emotional expressivity, sensitivity, and control. Using the measurements of handedness, we classified participants as being standard, mixed, or anomalous dominant. We presumed that hemispheric brain dominance and sex would interact to influence emotional expressivity, sensitivity, and control. Analysis revealed that brain dominance and sex interacted to influence negative emotional expressivity and emotional control. Specifically, mixed dominant (MD) males scored highest in negative emotional expressivity and lowest in emotional control, whereas MD females scored lowest on negative emotional expressivity and second highest on emotional control. In general, MD men and women reported behaviors opposite of traditional sex differences in emotional expression. 相似文献
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李先国 《衡阳师范学院学报》2001,22(5):65-67
对叙事作品形式技巧的重视,严格以时间为标准,使得当代叙事学的时序研究在传统叙事理论的叙述顺序研究的基础上,作出了独特的贡献。 相似文献
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胡习之 《阜阳师范学院学报(社会科学版)》2000,(4)
语言交际的主体是语言交际的决定性因素。本文从交际主体的角色约束,交际主体的形象魅力以及潜在交际主体等方面对之略作探讨,企望为语言交际美学的建立添砖加瓦。 相似文献
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真核生物表达系统研究进展 总被引:4,自引:0,他引:4
郑光宇 《喀什师范学院学报》2004,25(6):33-36
从毕赤酵母表达宿主菌,表达载体,外源蛋白在毕赤酵母中的表达,毕赤酵母表达系统的优化和丝状真菌的转化,CBH表达系统及rDNA整合序列等方面综述了近年来关于毕赤酵母和丝状真菌表达系统的研究进展. 相似文献