白藜芦醇联合野生型p53质粒诱导非小细胞肺癌H1299细胞凋亡

目的实验以非小细胞肺癌H1299细胞(简称H1299细胞)为研究对象,主要探讨白藜芦醇联合野生型p53质粒对H1299细胞凋亡作用的影响以及进一步探讨其作用机制。方法在H1299细胞中导入野生型p53质粒,加入不同浓度的白藜芦醇,通过MTT法确定白藜芦醇联合野生型p53质粒的最佳作用浓度,并检测白藜芦醇联合野生型p53质粒对细胞增殖的影响;光镜下观察细胞形态学的变化;流式细胞术检测白藜芦醇联合野生型p53质粒对H1299细胞凋亡率的影响;RT-PCR实验方法检测细胞凋亡相关基因Bcl-2、Bax、Caspase3、Caspase9的表达水平。结果白藜芦醇联合野生型p53质粒能明显抑制H1299细胞的增殖,诱导H1299细胞凋亡,明显抑制Bcl-2基因的表达水平,促进Bax、caspase3、caspase9基因的表达水平。结论白藜芦醇联合野生型p53质粒能够明显抑制H1299细胞的增殖,可能通过促凋亡基因Bax、凋亡蛋白酶Caspase3,Cas?pase9的表达,抑制抑凋亡基因Bcl-2的表达,从而激活细胞中线粒体介导的凋亡通路,诱导H1299细胞凋亡。     

International research networks in pharmaceuticals: Structure and dynamics

Knowledge production and scientific research have become increasingly more collaborative and international, particularly in pharmaceuticals. We analyze this tendency in general and tie formation in international research networks on the country level in particular. Based on a unique dataset of scientific publications related to pharmaceutical research and applying social network analysis, we find that both the number of countries and their connectivity increase in almost all disease group specific networks. The cores of the networks consist of high income OECD countries and remain rather stable over time. Using network regression techniques to analyze the network dynamics our results indicate that accumulative advantages based on connectedness and multi-connectivity are positively related to changes in the countries’ collaboration intensity whereas various indicators on similarity between countries do not allow for unambiguous conclusions.     

Strategic interactions in public R&D across European countries: A spatial econometric analysis

The paper adds to the existing literature on the determinants of government spending in Research and Development (R&D) by considering the role of strategic interactions among countries as one of the possible competing explanations, within a spatial econometric framework. We account for several factors affecting national levels of public R&D spending, including (i) the international context – i.e. Lisbon strategy; (ii) country characteristics – level of private R&D, GDP, trade openness and the National System of Innovation; (iii) countries’ similarities in relation to (a) trade and economic size and (b) sectoral specialization. The analysis is carried out on 14 European countries. First, we find that factors traditionally affecting the level of public R&D expenditure, such as the scale of the national economy, trade openness, sectoral specialization and private R&D, significantly influence the level of public R&D in European countries between 1994 and 2006. Interestingly, the introduction of the Lisbon strategy does not seem to have affected changes in the levels of public R&D spending. Second, by using different weight matrices, we confirm the existence of strategic interactions in relation to R&D spending among European countries with similar economic, international trade and sectoral structure characteristics, though not geographically close.     

Multinational subsidiary knowledge protection—Do mandates and clusters matter?

International knowledge spillovers, especially through multinational companies (MNCs), have recently been a major topic of academic and management debate. However, most studies treat MNC subsidiaries as relatively passive actors. We challenge this assumption by investigating the drivers of knowledge protection intensity of MNC subsidiaries. We argue that knowledge protection intensity is determined by MNC subsidiary mandates and by opportunities and risks originating from the host region. We hypothesize that not just competence-creating but also competence-exploiting mandates increase knowledge protection intensity. In addition, technological cluster regions in the host country can be expected to provide opportunities for knowledge sourcing and MNC subsidiaries may be willing to protect knowledge less intensively to participate in cluster networks. We test our hypotheses using a dataset of 694 observations of 631 MNC subsidiaries in Germany and develop recommendations for research, managers and policy makers.     

电子图书馆异构系统联合检索整合方法研究

首先提出当前电子图书馆由于系统异构造成的兼容问题,然后就Z39．50、OAI-PM H和OpenURL等协议及标准对整合异构系统及所含资源的作用和效果进行详细分析,并就使用OAI-PM H协议进行系统整合的步骤和方法加以说明,最后对电子图书馆的联合检索应用前景进行总结和展望。     

Address by H. E. LI Keqiang Premier of the State Council of the People's Republic of China At the 2014 Annual Meeting of the Global Research Council

<正>Beijing,27 May 2014Friends from the international science community,Ladies and Gentlemen,I am delighted to attend the 2014 annual meeting of the Global Research Council in Beijing.Established in 2012,the council is a young international science organization but has strong vitality and unlimited future.The council held its fi rst annual meeting in a developed country and is holding this year’s meeting in a developing one.This shows its global vision and dedication to openness and cooperation.The theme of this meeting,"Open Access and Shaping the Future",meets practical needs and reflects future trends.     

一类具有强奇性的矩阵型偏微分方程的正解的存在性

研究矩阵型强奇异偏微分方程 其中,Ω⊂Rⁿ是有界开集,M（x）是定义在Ω上的实对称矩阵,-p<-1,0 < q < 1,λ>0是参数,f（x）∈L¹（Ω）,f（x）>0 a.e.in Ω。证明,如果存在u₀ ∈H₀¹（Ω）满足∫_Ωf（x）|u₀|^1-pdx <+∞,则对任意的λ>0上述方程都有正H₀¹-解,即慢速解。我们注意到,对于奇异方程,古典解即C²（Ω）∩C（Ω）解不一定是H₀¹（Ω）解。     

多媒体视讯会议技术的分析与比较

多媒体视讯会议技术的发展经历了基于电路交换网络和基于lP网络的两个阶段,随着网络宽带技术的发展和普及,多媒体视讯技术的逐步成熟,人们对多媒体视频业务及应用的概念逐步了解,并在视讯会议、远程医疗、远程教育、远程监控、家庭娱乐、流媒体技术、移动视频通信等多个方面都有的广泛应用。本文分析了H.320、H.323、SIP三种视讯技术,然后比较了它们的差异,最后得出SIP协议必将是多媒体视讯会议的主宰。     

汇川H2U系列PLC之间RS-485通信的实现

主要研究了汇川公司H2U可编程控制器(PLC)的通信技术,实现了两个H2U系列PLC之间基于RS-485的通信,并详细介绍了PLC之间通信的硬件连接方式、通信串口参数配置、触摸屏画面的设计以及程序实现。所讨论的方法已在采用H2U PLC及辅助设备组建的"运动控制系统"课程的教学实验平台上进行了验证,可以拓展应用于多台H2U PLC之间以及其他设备与H2U PLC之间的RS-485通信实现,对工程应用具有一定的借鉴意义。