Mass spectrometry-based protein-protein interaction techniques and their applications in studies of DNA damage repair

Proteins are major functional units that are tightly connected to form complex and dynamic networks.These networks enable cells and organisms to operate properly and respond efficiently to environmental cues.Over the past decades,many biochemical methods have been developed to search for protein-binding partners in order to understand how protein networks are constructed and connected.At the same time,rapid development in proteomics and mass spectrometry(MS)techniques makes it possible to identify interacting proteins and build comprehensive protein-protein interaction networks.The resulting interactomes and networks have proven informative in the investigation of biological functions,such as in the field of DNA damage repair.In recent years,a number of proteins involved in DNA damage response and DNA repair pathways have been uncovered with MS-based protein-protein interaction studies.As the technologies for enriching associated proteins and MS become more sophisticated,the studies of protein-protein interactions are entering a new era.In this review,we summarize the strategies and recent developments for exploring protein-protein interaction.In addition,we discuss the application of these tools in the investigation of protein-protein interaction networks involved in DNA damage response and DNA repair.     

基于Halcon的光纤端面尺寸检测方法

针对传统光纤端面尺寸检测几何尺寸误差大、效率低的弊端,采用一种基于Halcon的光纤端面尺寸检测方法,利用数字图像处理算法,采用中值滤波的方法对图像进行预处理,消除图像噪声,并对图像进行二值化及形态学处理,选用Canny算子作为边缘检测算子确定像素级边缘,该方法边缘检测性能较好,且具有较强的抗噪声能力|再从选取的边缘中选出类圆度高的部分边缘进行共圆轮廓合并、拟合,通过拟合的椭圆和圆得到光纤半径、不圆度、同心度等几何参数。实验结果表明,纤芯和包层半径可以精确到万分之一,不圆度和同心度可以精确到小数点后8位,测量精度较高且不受操作水平影响。     

光纤通信实验教学的探索与实践

针对光纤通信课程理论性较强,知识面广,学生难掌握的特点,基于EL-GT-IV光纤通信教学实验系统设计了若干实验,并以一个具体实例来说明实践环节的具体实施。通过对所设计实验的开设激发了学生对理论知识的兴趣,便于学生更好地掌握光纤通信课程,深化课程教学的效果,提高他们的实践动手及创新能力。     

用于乙醇-水分离的SA-PVA/PSF中空纤维渗透汽化复合膜

Using polysulfone (PSF) hollow fiber ultrafiltration membranes as the substrate, sodium alginate (SA) and polyvinyl alcohol (PVA) blend solutions as the coating solution, and maleic anhydride (MAC) as the cross-linked agent, SAPVA/PSF hollow fiber composite membranes were prepared for the dehydration of ethanol-water. The effects of different sodium alginate concentration in the coating solutions and different operating temperatures on pervaporation performance were investigated. The experimental results showed that pervaporation performance of the SA-PVA/PSF composite membranes for ethanol-water solution exhibited a high separation factor although they had a relatively low permeation flux. As SA concentration in SA-PVA coating solution was 66.7% and the operating temperature was 40 ℃, SA-PVA/PSF hollow fiber composite membrane (PS4) had a separation factor of 886 and flux of 12.6 g/(m2·h). Besides, SA-PVA/PSF hollow fiber composite membranes (PS3 and PS4) were used for the investigation of the effect of ethanol concentration in the feed solution on pervaporation performance.     

近红外波段微结构光纤中光子带隙的实验研究

阐述了光子带隙的基本概念,以及微结构光纤中存在光子带隙的理论基础．用基于透射谱的方法测试了近红外波段800-3200nm范围内的三种微结构光纤,从透射谱图中发现有多条透射带,验证了光子带隙的存在．     

宽带光纤接入网的发展趋势

世界宽带接入网发展十分迅猛,亚太地区普及率居世界第一。宽带光纤接入网在点到点有源以太网系统和点到多点无源光网络具有诸多优点。     

蛇骨骼肌纤维型及运动终板的研究：4—Di—2—ASP荧光染色及计算机图像分析

用４－Ｄｉ－２－ＡＳＰ活性荧光染料染蛇腹横肌，清晰地显示三种肌纤维型及其运动终板，用ＨＰＳｃａｎＪｅｔ扫描仪对上述彩照进行高分辨扫描，得到良好的计算机数字图像，借助对相应参数的精确测定与分析，实现对形态学的直观定量表达     

偏振反射与二向性反射的关系 --以不同物候期杨树单叶的室内光谱测量为例

利用二向光度计实测了4个物候期杨树单叶的偏振反射比与二向反射比数据,并对此数据进行了分析,得出了杨树单叶的偏振反射特征与二向反射特征之间的关系及其对物候变化的响应,为偏振光遥感、多角度遥感的深入研究与应用提供了依据,并且对森林遥感也做出了一种新的尝试.     

用传输矩阵法研究光纤Bragg光栅的非线性特性

从耦合模理论出发，利用传输矩阵法求解耦合模方程，数值研究了光纤光栅双稳态开关特性，给出了输入输出特性的依赖关系曲线，得出一些有意义的结论。在此基础上，对传输矩阵法在啁啾光纤光栅的非线性双稳态中的应用进行了探讨。     

汽车自动变速器示教板的设计

利用单片机控制发光二极管和光纤来模拟汽车自动变速器的油路、阀体及其故障现象,采用键盘和LED显示器进行人机交互,成功地模拟了自动变速器的核心技术--自动换档功能.该软件设计操作方便简单,使学生能够清晰地领会自动变速器的工作原理.