Quantitative trait loci (QTL) analysis was conducted in bread wheat for 14 important traits utilizing data from four different mapping populations involving different approaches of QTL analysis. Analysis for grain protein content (GPC) sug- gested that the major part of genetic variation for this trait is due to environmental interactions. In contrast, pre-harvest sprouting tolerance (PHST) was controlled mainly by main effect QTL (M-QTL) with very little genetic variation due to environmental interactions; a major QTL for PHST was detected on chromosome arm 3AL. For grain weight, one QTL each was detected on chromosome arms 1AS, 2BS and 7AS. QTL for 4 growth related traits taken together detected by different methods ranged from 37 to 40; nine QTL that were detected by single-locus as well as two-locus analyses were all M-QTL. Similarly, single-locus and two-locus QTL analyses for seven yield and yield contributing traits in two populations respectively allowed detection of 25 and 50 QTL by composite interval mapping (CIM), 16 and 25 QTL by multiple-trait composite interval mapping (MCIM) and 38 and 37 QTL by two-locus analyses. These studies should prove useful in QTL cloning and wheat improvement through marker aided selection. 相似文献
An external quality assessment was conducted to assess the performance of various laboratories for RIA of thhyroid related
hormones in two phases. In the first phase thirty five laboratories participated. At the end of first phase a meeting cum
workshop was organised to discuss the results of first phase, difficultires faced by the participants and pinpoint the short
oming. A second phase was then initiated with an objective of improvement in the performance, if any, where twelve samples
from four pools were distributed to twenty four laboratories who participated for the second phase. The overall return of
the results increased significantly from 71.8% (1586/2208) for the first phase to 92.4% (732/792) for the second phase. The
inter laboratory %CV for T3, T4 and TSH were lower during the second phase (30.6%, 19.0% and 31.6% respectively) as compared to those observed during first
phase (36.3%, 22.7% and 52.8% respectively). Similarly, there was an improvement in reproducibility of ALTM as %CV for T3, T4 and TSH decreased from 6.0%, 9.8% and 13.4% respectively to 4.5%, 4.6% and 8.5% respectively. The individual performances
of the participating laboratories viz. bias, variability of bias and imprecision also showed a trend towards improvement as
percent laboratories having desirable or acceptable results for T3, T4 and TSH increased from 10.7%, 60.7% and 0.0% respectively to 20.8%, 66.7% and 22.2% respectively. External quality assessment
thus appears to be beneficial in assessing the performane of a laboratory in comfparison with other laboratories and indeed
helps in improving the performance. 相似文献
Protein fraction termed P2 was obtained from sonicates of Mycobacterium smegmatis, subjected to ammonium sulphate precipitation. P2 fraction was further fractionated into 4 fractions (PeakI-IV) by DEAE cellulose ion-exchange chromatography. ELISA was performed
on the sera of 104 tuberculous cases and 62 controls, using P2 fractions Peak I as test antigens. Though the mean ELISA values in tuberculous cases were higher than the controls, no statistically
significant difference was found between the two. Antigens P2, Peak I and IV were tested on Western blots with pooled sera from tuberculous cases as well as controls. P2 fraction, Peak-I and IV were separated on PAGE-SDS, electroblotted onto nitrocellulose Sheets and the blots were subjected
to ELISA. Peak IV appeared as a single band (M.W. 55,000). P2 fraction exhibited some discriminatory bands on development of blots. 相似文献
This paper investigates the development of university spinout companies (USOs). Employing a case-based research method, our study found that there are two important elements in their development. First, USOs go through a number of distinct phases of activity in their development. Each venture must pass through the previous phase in order to progress to the next one but each phase involves an iterative, non-linear process of development in which there may be a need to revisit some of the earlier decisions and activities. Second, at the interstices between the different phases of development we found that ventures face “critical junctures” in terms of the resources and capabilities they need to acquire to progress to the next phase. The different phases are critical as these ventures cannot develop into the next phase without overcoming each of the junctures. We identify four different critical junctures that spinout companies need to overcome if they are to succeed: opportunity recognition, entrepreneurial commitment, credibility and sustainability. 相似文献
The cause of more insulin resistance in female than males are still unknown. To know the cause from early life, normal values of relevant parameters are required. So, aim of this study was to determine the reference levels of glucose and insulin in cord blood of term newborns and to examine their effects on gender, placental and birth weight of term newborns. In cross sectional study 60 consecutive term newborns were included from constituent hospitals. Placental and birth weights were measured and cord blood was collected for estimation of serum insulin and plasma glucose. Plasma glucose estimation was done by auto analyzer (GOD–POD method) and serum insulin analysis was done using Insulin ELISA Kit. After analysis, mean ± 2SD used for estimating cord blood insulin and glucose levels, which were 10.1 ± 7.8 μIU/mL and 67.8 ± 33.8 mg/dL respectively. Correlation of insulin with both birth weight and placental weight were r = 0.359 and 0.41 respectively; p < 0.001. Interestingly we found higher insulin levels in females as compared to male newborns in spite of having lower birth weight in them. In conclusion this study reported the levels of insulin and glucose in cord blood of term newborns. Incidentally, this is the first study as per our knowledge to report significant correlation between cord blood insulin, glucose with birth weight, placental weight and gender in south India. Female newborns had higher insulin levels than males, despite lesser birth weight can be attributed to intrinsic insulin resistance in them. 相似文献
Purpose: The key informants in a village setup were studied to understand the existing social and knowledge systems of farmers, their structure, and relationships between different actors. The purpose is to identify different channels of information and use them as a means to disseminate agricultural technologies and related information to farmers.
Design: We use the network map analysis in a case study approach as a tool to demonstrate the linkages between the key actors and stakeholders in the information network of farmers.
Findings: The government institutions are well networked among themselves but have limited interactions with non-government sources. Farmers in Bihar have strong linkages with few network actors, who are important nodes in the social knowledge network. The study showed heterogeneity and complexity in the network shape and structure across different districts.
Practical Implications: Knowledge networks and social networks are the drivers of information sharing and play a significant role in the diffusion of agricultural technology and related knowledge. Understanding these networks provides a platform for introducing the agricultural technologies and getting connected to a wider group of farming communities.
Theoretical Implications: The study shows that the network is formed by different actors and their role determine the nature and shape of the network. Knowledge of this heterogeneity is important in designing or revamping agricultural information systems.
Originality: Information and knowledge networks are least explored in the agricultural information dissemination process. Some studies have shown the role of actors and social networks, but this study uniquely explores and presents the heterogeneity of these networks. 相似文献
According to similarity property principle, structurally similar molecules tend to have similar properties. Similar molecules
exhibit similar biological activities. However, there is no hard and fast rule that the compounds with similar chemical structure
will have similar functions. There are several compounds of similar chemical structure with significantly different biological
actions and activities. Structure similarity of natural products can be investigated using Tanimoto coefficient and Euclidean
distance measurements. To do this, the molecules are decomposed into smaller fragments, and a dictionary, of the fragments
are prepared. Using the dictionary, 2D fingerprints are formed. With the fingerprints Tanimoto coefficient and Euclidean distance
are quantitated. 相似文献