Poly A RNA status and expression of milli (m) and micro (μ) calpains in skeletal muscle of duchenne muscular dystrophy patients

Poly A RNA status and itsin vitro translation in a rabbit reticulolysate cell free system, were assessed in the skeletal muscle of young boys with Duchenne muscular dystrophy. Compared to normals there is a significant 48% increase in poly A RNA content of dystrophic muscle and its translatability was increased by 56% based on³⁵S methionine incorporation into total protein systhesised. Immunoprecipitation of the translated products with monospecific antibodies showed that there is a 2.6 fold and a 2 fold increase in m and μ calpains respectively. This underlines the importance of both synthetic and degenerative activities in the early pathology in DMD muscle.     

Innovations in university finance: A growing necessity,and intelligent too!

In increasing numbers, public and particularly private colleges and universities are facing financing crunches associated with declining enrollments, cuts in federal aid programs, reduced grant pools, etc. These financing challenges are evident not only in terms of capital investment needs but also short-term operating demands. However, universities and colleges do have vehicles available to them to deal with both short-term and long-term needs—in the debt markets. Variable rate demand bonds and tax-exempt variable rate demand bonds are currently being marketed for just such purposes. In addition, the future appears bright for a currently unavailable instrument—real rate bonds which could further reduce the financing expenses for schools. The characteristics, advantages, and disadvantages of each are discussed along with some examples of their superiority to alternative financing.     

Relationships between sixth-graders’ reading comprehension and two different measures of print exposure

This study examined sixth-graders’ reading comprehension and component reading abilities in relation to two measures of print exposure: an author recognition test (ART) involving fiction authors and a reading habits questionnaire (RHQ) about children’s voluntary reading for enjoyment across various genres. The ART correlated only with children’s fiction book reading habits, not with other habits such as nonfiction book or magazine reading, and had a stronger relationship to all tested reading abilities than did the RHQ. Strong comprehenders in reading outperformed weak comprehenders on all component reading measures, ART score, and fiction habits; however, weak comprehenders scored higher than did strong comprehenders on the indicator of nonfiction reading habits. The two groups of comprehenders did not differ significantly on other reported reading habits. The results are discussed in relation to children’s specific book choices and demonstrate the relevance of genre to evaluations of children’s print exposure.     

Teachers’ literacy-related knowledge and self-perceptions in relation to preparation and experience

After rating their own literacy-related knowledge in three areas (knowledge about reading/reading development, phonemic awareness/phonics, and morpheme awareness/structural analysis), graduate teacher-education students completed five tasks intended to measure their actual disciplinary knowledge in these areas. Teachers with high levels of prior background (i.e., course preparation and experience) rated themselves as significantly more knowledgeable than did low-background teachers in all areas; high-background participants also significantly outperformed low-background participants on all tasks. However, even high-background teachers scored well below ceiling on the tasks. Regression analyses indicated that teachers’ self-perceptions and knowledge were positively influenced by both level of preparation and teaching experience, although the influences on teachers’ knowledge differed by task. Teachers had some accurate perceptions of their own knowledge, especially in the area of phonics. Results suggest that differentiating levels of preparation may be useful in studying teacher knowledge, and also support the notion of a substantial gap between research on reading and teacher preparation in reading.     

An evaluation of consultation sessions for foster carers and adoptive parents

The aim of the study was to examine whether consultation sessions provided by educational psychologists (EPs) offer a useful way of supporting foster and adoptive parents. Feedback was collected from 101 EPs and 78 foster and adoptive parents through the use of a short questionnaire at the end of each session. As might be expected, educational issues were the main focus of the sessions, although other areas such as behaviour management and emotional well-being were also addressed. In general, both EPs and carers were enthusiastic about the sessions, with carers reporting that they found it helpful to be able to discuss their concerns, receive practical advice, set goals and gain a new insight into their situation. These findings were supported by an increase in carers’ feelings of confidence, and a decrease in their feelings of concern, following the sessions. Such findings endorse the use of EP-led consultation sessions as a means of supporting foster and adoptive parents.     

Detection of diagenetic alterations by Spectroscopic Analysis on Archaeological Bones from the Necropolis of Poseidonia (Paestum): A case study

Understanding the chemical and physical alteration in archaeological bones, occurred after burial, is very interesting for researchers. In this paper, we present a study on the diagenetic alteration of human archaeological bony tissues from Paestum (South Italy), by combining complementary spectroscopic techniques, such as solid-state nuclear magnetic resonance (NMR), infrared spectroscopy (IR), and X-ray diffraction. In particular, ¹³C nuclear magnetic resonance–cross polarization-magic angle spinning (¹³C NMR CP-MAS) spectroscopy allows to identify and discriminate the adsorbed calcite, that is a diagenetic contaminant, from the structural one of apatite and ¹H NMR-MAS spectroscopy shows how the degradation of organic phase of collagen is related to the time. The NMR data are combined with crystalline index, measured by X-ray diffraction, and with the splitting factor obtained by infrared spectroscopy. Moreover, the evaluation of the relative content of biogenic structural carbonate and of diagenetic fluorine is reported.     

Institutional repositories: is the open access door half open or half shut?

The response of the UK Government to the Report on science publishing by the Science and Technology Select Committee of the House of Commons is criticized for failing to take the necessary action to research or help catalyse a change in the publishing model. The initiatives of the National Institutes of Health and the Wellcome Foundation in setting up central archives are examined and their utility questioned. The evidence base for a change to open access is explored.     

Analysis of Bcl I and Xba I polymorphism in factor VIII gene to detect carriers of haemophilia a in Andhra Pradesh

The efficacy of two intragenic polymorphic markers of factor VIII gene has been examined in Andhra Pradesh population with a view to confirm/revise the strategy for carrier detection that would be precise and economical. The haemophilia A carrier was detected using Bcl I and Xba I polymorphic sites in intron 18 and 22 respectively. The cumulative efficiency of these two sites for detection of carriers is 100% since all 15 families tested were informative for one of these polymorphisms, thus confirming their usefulness for factor VIII gene mutations found in Andhra Pradesh.