Effect of diabetic serum factor on polymorphonuclear leucocyte membrane hydrophobicity and particle internalisation

Membrane hydrophobicity and slalidase activity of normal Poly morphonuclear Leucocyte were significantly enhanced when incubated with DSF. As a consequence, internalisation ofE. coli andS. aureus (opsonised or unopsonised) were greatly dimnished, internalisation ofE. coli being higher in either category. Although, increase in hydrophobicity of the membrane correlated well with the time of decrease of particle internalisation (both at 30 min.), enhancement of sialidase activity did not coincide with the said alterations.     

Let the Germans Win? Israeli Attitudes towards Die Mannschaft during The 2014 World Cup Tournament

To contribute to analyses of the sport-politics nexus, the study examines Jewish-Israeli attitudes toward the German soccer team and Germany. The analysis reveals that more Israelis root against the German soccer team than support it, despite the team’s impressive achievements. The analysis of attitudes toward Germany surprisingly reveals that the 18–20 age group expressed the strongest animosity towards Germany, which may indicate that Israeli government attempts to instill the Holocaust memory in the young generations perpetuates animosity between young Israelis and Germany. The study strengthens the notion that international sport games represent and extend rather than reduce international tensions.     

Experimental therapeutic intervention with alpha tocopherol in ethanol induced testicular injuries in rats

Infertility is well-established harmful effect in chronic alcoholism and so far, there is no effective treatment for this condition. The study was conducted to determine the effects of alpha tocopherol on ethanol induced testicular injuries in male albino rats of Wistar strain. Five groups (n=6) of animals were used. Group I served as control. Group II received daily 1.6g ethanol/kg body weight/day for 4 weeks orally. Group III received 1.6g ethanol+80mg alpha tocopherol/kg body weight/day for four weeks orally. Group IV received 1.6g ethanol/kg body weight for/day 4 weeks and followed by 80mg alpha tocopherol/kg body weight/day for four weeks orally. Group V received 1.6g ethanol/kg body weight/day orally for 4 weeks, followed by 4 weeks abstinence. Twently-four hours after the last treatment the rats were sacrificed using anesthetic ether. Testes were removed and used for the estimation of extent of lipid peroxidation and tissue levels of antioxidants and steroidogenic enzymes. Alpha tocopherol treatment increased the activities of testicularΔ ⁵, 3β-HSD. Moreover, the treatment was also associated with significant decrease in testicular oxidative stress. Ethanol-induced oxidative stress and decreased steroidogenesis can be reversed by treatment with alpha tocopherol.     

Adiponectin Gene Polymorphism and its Association with Type 2 Diabetes Mellitus

Mutations in different regions of adiponectin gene have been reported to be associated with obesity, atherosclerosis and type 2 diabetes mellitus. The present study was aimed to investigate the association among SNP 45 T > G of adiponectin gene and type 2 diabetes in South Indian population. 75 clinically diagnosed case of type 2 diabetes were studied and compared with 75 apparently healthy controls. The genotype frequency of SNP45 T > G in exon 2 of adiponectin gene was determined by PCR based restriction enzyme analysis using the restriction enzyme SmaI. (recognition site: CCC↓GGG). Three kind of genotypes: wild type TT (470 bp), heterozygous type TG (470 bp, 336 bp, 134 bp) and homozygote mutant type GG (336 bp, 134 bp) were studied. A positive association has been found between SNP45 T > G and type 2 diabetes in the study population (P = 0.010, OR = 3.797, 95% CI = 1.312–10.983). Therefore, SNP45T > G in adiponectin gene may be one of the risk factors for type 2 diabetes.     

Bromo-oxidation reaction in enzyme-entrapped alginate hollow microfibers

In this article, the authors present the fabrication of an enzyme-entrapped alginate hollow fiber using a microfluidic device. Further use of enzyme-entrapped alginate hollow fibers as a biocatalytic microchemical reactor for chemical synthesis is also deliberated in this article. To ensure that there is no enzyme leaching from the fiber, fiber surfaces were coated with chitosan. To confine the mobility of reactants and products within the porous hollow fibers the entire fibers were embedded into a transparent polydimethylsiloxane (PDMS) matrix which also works as a support matrix. A vanadium-containing bromoperoxidase enzyme isolated from Corallina confusa was used as a model enzyme to demonstrate the use of these alginate hollow-fiber reactors in bromo-oxidation of phenol red to bromophenol blue at different dye flow rates. Stability of the entrapped enzyme at different temperatures and the effect of the chitosan coating on the reaction conversion were also studied. It was observed that molecules as big as 27 kDa can be retained in the matrix after coating with chitosan while molecules with molecular-weight of around 378 Da can still diffuse in and out of the matrix. The kinetic conversion rate in this microfluidic bioreactor was more than 41-fold faster when compared with the standard test-tube procedure.     

Analyzing the temporal evolution of students’ behaviors in open-ended learning environments

Metacognition and self-regulation are important for developing effective learning in the classroom and beyond, but novice learners often lack effective metacognitive and self-regulatory skills. However, researchers have demonstrated that metacognitive processes can be developed through practice and appropriate scaffolding. Betty’s Brain, an open-ended computer-based learning environment, helps students practice their cognitive skills and develop related metacognitive strategies as they learn science topics. In this paper, we analyze students’ activity sequences in a study that compared different categories of adaptive scaffolding in Betty’s Brain. The analysis techniques for measuring students’ cognitive and metacognitive processes extend our previous work on using sequence mining methods to discover students’ frequently-used behavior patterns by (i) developing a systematic approach for interpreting derived behavior patterns using a cognitive/metacognitive task model and (ii) analyzing the evolution of students’ frequent behavior patterns over time. Our results show that it is possible to identify students’ learning behaviors and analyze their evolution as they work in the Betty’s Brain environment. Further, the results illustrate that changes in student behavior were generally consistent with the scaffolding provided, suggesting that these metacognitive strategies can be taught to middle school students in computer-based learning environments.     

Rapid and cost-effective fabrication of selectively permeable calcium-alginate microfluidic device using ��modified�� embedded template method

In this paper, we have presented a non-lithographic embedded template method for rapid and cost-effective fabrication of a selectively permeable calcium-alginate (Ca-alginate) based microfluidic device with long serpentine delay channel. To demonstrate the versatility of the presented method, we have demonstrated two different strategies to fabricate serpentine long delay channels without using any sophisticated microfabrication techniques, in formal lab atmosphere. The procedure presented here, also, enables the preparation of a multilayered microfluidic device with channels of varying dimensions, in a single device without using any sophisticated micromachining instrumentation. In addition, we have also qualitatively studied the diffusion of small and large molecules from a Ca-alginate based microfluidic device and proposed a method to effectively control the out-flow of macro biomolecules from the crosslinked Ca-alginate matrix to create a selectively permeable matrix required for various biological and biomimetic applications, as mentioned in the Introduction section of this work.     

Current status,enablers ＆ barriers of implementing cellular manufacturing system in Indian industries

The cellular manufacturing （CM） has been proved as a well-known manufacturing strategy that helps to improve manufacturing efficiency and productivity by utilizing the philosophy of group technology. Large num- ber of papers has been published in the area of design issues of CM system. Unfortunately, the issues related to acceptability of CM in Indian industries are typically not examined rigorously as technical issues. This paper pre- sents the results of a survey carded out to find the status, enabler and barrier of implementing CM system in Indian industries.     

An integrated and open-ended experiment

In this article we discuss an exciting experiment in non-linear dynamics. This provides an imaginative platform for bringing in chemical, physical, biological, mathematical and computational sciences together. There are implications for earth sciences as well. B M Deb is a theoretical chemist who has been designing experiments for chemistry teaching laboratories for more than thirty years. Mainak Sadhukhan, Sudarson Sekhar Sinha and Sucheta Sengupta were students of PBIR-Chemical Sciences. Ranjit Biswas is a physical chemist working on both theory and experiment in chemical dynamics.