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Hadi Yaghoubinejad Nourollah Zarrinabadi Dariush Nejadansari 《Teachers and Teaching》2017,23(2):127-140
Research has shown that lack of motivation on either students or teachers’ part negatively influences language learning. The latter case is a serious problem in this regard, since an unmotivated teacher will extinguish his learners’ enthusiasm and energy for learning. This study employed a mixed-method approach to investigate the demotivating factors for Iranian junior high school teachers. For the quantitative stage of the study, a questionnaire on demotivation was distributed among 105 participant teachers to find out the dominant demotivating factors. In the qualitative stage, and in order to gain a better picture of the salient demotivating factors and the reasons behind them, a set of semi-structured interviews with 10 volunteer teachers were conducted. The results indicated that lack of social recognition and respect, few adequate rewards, lack of supports or understanding regarding English education, and a large number of students in a single English class were the major demotivating factors for Iranian teachers. In the interviews, it was found that the participants perceived the first two items as specific to Iranian educational context. Finally, the implications for policy-makers and material developers and suggestions for future researchers are discussed. 相似文献
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Ali Farhangi Dariush Norouzian Mohammad Reza Mehrabi Mohsen Chiani Zahra Saffari Maryam Farahnak Azim Akbarzadeh 《Indian journal of clinical biochemistry : IJCB》2014,29(4):406-417
The objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult Wistar rats via comparison of changes in body weight, consumption of food, volume of water, urine and levels of glucose, insulin and C-peptide in serum, between normal and diabetic rats. Intra-venous injection of 60 mg/kg dose of streptozotocin in 250–300 g (75–90 days) adult Wistar rats makes pancreas swell and causes degeneration in Langerhans islet β-cells and induces experimental diabetes mellitus in 2–4 days. For a microscopic study of degeneration of Langerhans islet β-cells of diabetic rats, biopsy from pancreas tissue of diabetic and normal rats, staining and comparison between them, were done. In this process, after collagenase digestion of pancreas, islets were isolated, dissociated and identified by dithizone method and then with enzymatic procedure by DNase and trypsin, the islet cells changed into single cells and β-cells were identified by immune fluorescence method and then assayed by flow-cytometer. Donor tissue in each step of work was prepared from 38 adult male Wistar rats weighted 250–300 g (75–90 days). Transplantation was performed in rats after 2–4 weeks of diabetes induction. In this study, the levels of insulin, C-peptide and glucose in diabetic rats reached to normal range as compared to un-diabetic rats in 20 days after transplantation of islet cells. Transplantation was performed under the cortex of testis as immunoisolated place for islet cells transplantation. 相似文献
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Azim Akbarzadeh Dariush Norouzian Ali Farhangi Mohammad Reza Mehrabi Shirin Jamshidi Davood Zare Morvarid Shafiei 《Indian journal of clinical biochemistry : IJCB》2008,23(1):57-61
Flow cytometry has been employed as a method to study homogeneity of isolated islet subpopulations. After collagenase digestion
of rat pancreas and elutriation of tissue fragments, islets were isolated and dissociated, and cells were analyzed and sorted
according to their low forward angle light scattering properties by using automated flow cytometry. A standardized procedure
was developed for the preparation of rat islet cell grafts for purification of islet cells. In this process, after collagenase
digestion of pancreas, islets were isolated, dissociated, identification by dithizone method and then with enzymatic procedure
by DNase and trypsin, the islet cells changed into single cells and beta cells were identified by immunofluorescence method
and then assayed by flow cytometry. Methods have been developed for the preparation of suspension of viable rat pancreatic
islet cells and their analysis and sorting in the fluorescence activated cell sorter (FACC IV, Becton Dickinson, Sunnyvale,
Ca). Flow cytometry of these cells indicated that there were 91% of beta cells in cell suspension. Most of the exocrine particles
were lost during digestion. Purified endocrine islet cell grafts were prepared by pure beta-cells, without endocrine non-beta
cells. The purified aggregates were devoid of endocrine non-beta cells and damaged cells. 相似文献
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Mitra Salehi Shahin Amiri Dariush Ilghari Lawahidh Fadhil Ali Hasham Hossein Piri 《Indian journal of clinical biochemistry : IJCB》2023,38(2):159
The respiratory symptoms of acute respiratory distress syndrome (ARDS) in the coronavirus disease 2019 (COVID-19) patients is associated with accumulation of pre-inflammatory molecules such as advanced glycation end-products (AGES), calprotectin, high mobility group box family-1 (HMGB1), cytokines, angiotensin converting enzyme 2 (ACE2), and other molecules in the alveolar space of lungs and plasma. The receptor for advanced glycation end products (RAGEs), which is mediated by the mitogen-activated protein kinase (MAPK), plays a critical role in the severity of chronic inflammatory diseases such as diabetes mellitus (DM) and ARDS. The RAGE gene is most expressed in the alveolar epithelial cells (AECs) of the pulmonary system. Several clinical trials are now being conducted to determine the possible association between the levels of soluble isoforms of RAGE (sRAGE and esRAGE) and the severity of the disease in patients with ARDS and acute lung injury (ALI). In the current article, we reviewed the most recent studies on the RAGE/ligands axis and sRAGE/esRAGE levels in acute respiratory illness, with a focus on COVID-19–associated ARDS (CARDS) patients. According to the research conducted so far, sRAGE/esRAGE measurements in patients with CARDS can be used as a powerful chemical indicator among other biomarkers for assessment of early pulmonary involvement. Furthermore, inhibiting RAGE/MAPK and Angiotensin II receptor type 1 (ATR1) in CARDS patients can be a powerful strategy for diminishing cytokine storm and severe respiratory symptoms. 相似文献
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Simin Sohrabi Azim Akbarzadeh Dariush Norouzian Ali Farhangi Mehri Mortazavi Mohammad Reza Mehrabi Mohsen Chiani Zahra Saffari Soheil Ghassemi 《Indian journal of clinical biochemistry : IJCB》2011,26(4):354-359
The attempt is made to produce recombinant factor VIII but the first step in producing such product is production and purification
of rabbit’s polyclonal antibody against factor VIII. The second and third steps involve monoclonal antibody and recombinant
factor VIII production. Factor VIII is one of the most important coagulating factor where its deficiency leads to diseases
like hemophilia type A or classic. It is an inherited disease. Previously, it was obtained through fractionation of blood
plasma of blood donors. After processing, factor VIII could be used to manage such patients. Due to transfer of viral disease
like hepatitis and HIV through factor VIII obtained by fractionation, high cost of production, insufficiency of the donors
and the process of virus removal, thus production of factor VIII through recombinant technology can be useful and helpful.
The reaction between antibody and antigen is one the most specific reaction; therefore, such reaction can be employed to identify
factor VIII. Thereby, rabbits were injected several times with adjuvant-linked antigen to produce antibody. The antibody was
separated from the blood sample, purified and used to identify factor VIII in the research. 相似文献
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