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Ming-Cheng Lin Jiraporn Nawarak Tai-Yuan Chen Hsien-Yu Tsai Jung-Feng Hsieh Supachok Sinchaikul Shui-Tein Chen 《Biomicrofluidics》2009,3(3)
Rapid detection of brain natriuretic peptide (BNP) concentration can be used for the diagnosis of acute heart failure and for the evaluation of the effectiveness of a clinical therapy. We used the systematic evolution of ligands by exponential enrichment method to develop DNA aptamers for BNP whose sequences were determined by cloning method and consensus sequence analysis. A total of eight conserved sequences was identified. By combining the fluorescent-labeled aptamers with fast protein lab-on-chip analysis, we could achieve quantification of BNP concentrations with high speed, sensitivity, and specificity. 相似文献
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基于对留学泰国的中国留学生的调查数据,验证了所学专业和家庭背景会影响到学业失败,以及在学业失败上存在着性别差异的假设。进一步的研究发现,学业失败的性别差异不仅在泰国语专业中得到了扩大,而且也受到父亲职业的影响:相对而言,个体户的儿子更多地挂科,个体户的女儿则更少挂科。建议从性别角色的角度寻找导致性别差异随所学专业和父亲职业变化的原因。 相似文献
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