Holocaust education in Austria: A (hi)story of complexity and ambivalence

In Austria, activities for teaching about and remembering the Holocaust have concentrated mainly on National Socialism and its atrocities. Austria’s history of political anti-Semitism goes back to the 19th century, however, and has been widely and publicly acknowledged. It has always been linked to nationalistic tendencies that are still present today and rarely reflected upon, including the anti-Slavic and anti-Turkish attitudes that right-wing parties use to gain supporters. Vienna’s special place of remembrance, the Heldenplatz, with its monuments and history, is a useful place to begin examining Austrian identities and the course of collective Austrian ways of thinking. Based on that examination, we then consider Austria’s daily politics and treatment of the past. We next turn to Holocaust education after the war, which has had an impressive impact after a late start, and mention some of its drawbacks and problems. We next discuss the lack of serious research about memorials in Austria, as compared with Germany, and present initial results from a project that started in spring 2009 to examine knowledge gains and attitude changes among students after they visit the Mauthausen concentration camp.     

Enrichment of live unlabelled cardiomyocytes from heterogeneous cell populations using manipulation of cell settling velocity by magnetic field

The majority of available cardiomyocyte markers are intercellular proteins, limiting our ability to enrich live cardiomyocytes from heterogeneous cell preparations in the absence of genetic labeling. Here, we describe enrichment of live cardiomyocytes from the hearts of adult mice in a label-free microfluidic approach. The separation device consisted of a vertical column (15 mm long, 700 μm diameter), placed between permanent magnets resulting in a field strength of 1.23 T. To concentrate the field at the column wall, the column was wrapped with 69 μm diameter nickel wire. Before passing the cells through the column, the cardiomyocytes in the cell suspension had been rendered paramagnetic by treatment of the adult mouse heart cell preparation with sodium nitrite (2.5 mM) for 20 min on ice. The cell suspension was loaded into the vertical column from the top and upon settling, the non-myocytes were removed by the upward flow from the column. The cardiomyocytes were then collected from the column by applying a higher flow rate (144 μl/min). We found that by applying a separation flow rate of 4.2 μl/min in the first step, we can enrich live adult cardiomyocytes to 93% ± 2% in a label-free manner. The cardiomyocytes maintained viability immediately after separation and upon 24 h in culture.