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应用Con-A亲和层析方法从两株Den2病毒(New Guinea C株和从中国海南省分离的H-87株)感染的C6/36细胞膜中分离的糖蛋白,发现了一种感染细胞中特有的蛋白质。经SDS-PAGE证明该蛋白分子量约46000D,分析其为Den 2病毒的非结构蛋白NS1。并利用Western blot筛选出针对该蛋白4种单克隆抗体。 相似文献
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毛泽东同志根据中国国情,吸收传统思想精华,把农业放到异乎寻常的重要位置,在发展农业方面提出了很多真知灼见。但是我国农业始终发展不快,值得我们深思并总结经验教训。 相似文献
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文中用Galerkin方法和先验估计证明了带有阻尼项的RLW方程周期边界问题周期强解的存在唯一性,并在较弱的光滑性条件下,得到了强解的光滑性。 相似文献
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郭莉萍 《辽宁广播电视大学学报》2001,(4):65-67
中国共产党早期阶级分析思想的形成和发展是一个客观过程,是集体智慧的结晶。其中,毛泽东的分析更具有科学性和正确性。 相似文献
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Ye Q Qiu YX Quo YQ Chen JX Yang SZ Zhao MS Fu CX 《Journal of Zhejiang University. Science. B》2006,7(11):868-872
Sinocalycanthus chmensis, an endangered species endemic to China, is cultivated as an omamental landscape tree in China. However, S. chinensis, Chimonanthus species and Calycanthusfloridus are difficult to be distinguished in seedling market because of their similar morphological characters. In this study, ISSR (inter-simple sequence repeats) were applied to detect S. chinensis from its closely related species. A unique 748-bp band was found in all accessions of S. chinensis. SCAR (sequence characterized amplified regions) markers were created by cloning and sequencing the specific band, and designing a pair of primers to amplify the band of 748 bp. Diagnostic PCRs were performed using the primer pair with the total DNAs ofS. chinensis, Chimonanthus species and C. floridus as templates, with only S. chinensis being able to be amplified. This amplification is not only rapid (results can be obtained in less than 3 h), but is also easy to perform. Hence it is a feasible method for identifying S. chmensis in seedling market. 相似文献
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