Quantitation of endogenous inhibitor of milli-CANP in Duchenne muscular dystrophy platelets and lymphocytes: Its implication in regulation

With a view to gain an insight into the regulation of calcium activated neutral protease by its endogenous inhibitor, the inhibitor was quantitated in platelets and lymphocytes of Duchenne muscular dystrophy patients and controls by dot immunoblot procedure using polyclonal antibodies against the inhibitor. A two fold increase in inhibitor levels was observed in lymphocytes but no significant increase in inhibitor levels in platelets from Duchenne muscular dystrophy. These results suggest that different regulatory mechanisms of modulation of CANP operate in these two cell types in Duchenne muscular dystrophy.