1,2—二甲基肼的合成和致癌试验

1,2—二甲基肼(1,2—Dimethylhydrazine,DMH)具有强烈的致癌作用,并有高度的器官专一性,能特异地诱发啮齿动物大肠癌。在肿瘤生化研究中,为了获得理想的大肠癌动物模型,我们用Hatt法进行该药物的合成工作,并对合成产物—DMH作了小鼠致大肠癌的试验。 DMH的合成程序是:1.在碱性条件下,苯甲酰氯与硫酸肼反应,生成二苯甲酰肼;     

Purification and relationship with gastric disease of a 130 kDa (CagA)protein of Helicobacter pylori

ＩＮＴＲＯＤＵＣＴＩＯＮＨｅｌｉｃｏｂａｃｔｅｒｐｙｌｏｒｉｉｎｆｅｃｔｉｏｎｈａｄｂｅｅｎｒｅｃｏｇ ｎｉｚｅｄａｓａｎｉｍｐｏｒｔａｎｔｃａｕｓａｔｉｖｅｆａｃｔｏｒｉｎｃｈｒｏｎｉｃｇａｓｔｒｉｔｉｓａｎｄｐｅｐｔｉｃｕｌｃｅｒｄｉｓｅａｓｅ (ＰＵＤ     

Purification and relationship with gastric disease of a 130 kDa (CagA) protein of Helicobacter pylori

Objective: The aims of this research were to purify and identify the 130 kDa (CagA) protein of H. pylori clinical isolate HP97002 and evaluate the relationships between the purified 130 kDa (CagA) protein and gastric diseases. Methods: The procedure for isolating the protein included 6 mol/L guanidine extract, size exclusion chromatography and elusion from gel. Sera of 68 patients with gastric diseases (44 with chronic gastritis,15 with atrophic gastritis,7 with peptic ulcer disease,2 with gastric cancer ) were obtained, and the serological response to CagA was studied by Western-blot using the purified protein. Results: The purified protein was 130 kDa and preserved good antigenicity and revealed basic isoelectric point about of 8.1. Among 68 sera, 43 sera could recognize the purified protein associated with chronic gastritis 47.7% (21/44),atrophic gastritis 86.7% (13/15),peptic ulcer disease 100% (7/7),gastric cancer 100% (2/2). Compared with each other, the difference was significant (χ2=13.327, P=0.004), and 130 kDa (CagA) protein was associated with severe gastric diseases (rs=0.442, P=0.001). Conclusion: The 130 kDa (CagA) protein was associated with severe gastric diseases.