Robotic automation of droplet microfluidics

Droplet microfluidics enables powerful analytic capabilities but often requires workflows involving macro- and microfluidic processing steps that are cumbersome to perform manually. Here, we demonstrate the automation of droplet microfluidics with commercial fluid-handling robotics. The workflows incorporate common microfluidic devices including droplet generators, mergers, and sorters and utilize the robot''s native capabilities for thermal control, incubation, and plate scanning. The ability to automate microfluidic devices using commercial fluid handling will speed up the integration of these methods into biological workflows.     

Evaluation of evidence-based medicine search skills in the clinical years

The librarians of the Health Sciences Library worked with the director of the Primary Care Clerkship to reinforce the principles of Evidence-Based Medicine (EBM) searching, taught during the first two years of medical school, through an intensive workshop. The purpose of the program was to ensure that students apply EBM principles in a timely and effective manner in clinical situations. Working in teams led by a resident and librarian, students researched real cases and then evaluated the effectiveness of their approach to the problems. This paper outlines the rationale for the team approach, reviews the administration of a computer-based workshop, and discusses the evaluation process. Evaluation focused on both the current workshop and its implications for the informatics program presented to students during years one and two.     

Electrical lysis of cells for detergent-free droplet assays

Efficient lysis is critical when analyzing single cells in microfluidic droplets, but existing methods utilize detergents that can interfere with the assays to be performed. We demonstrate robust cell lysis without the use of detergents or other chemicals. In our method, cells are exposed to electric field immediately before encapsulation in droplets, resulting in cell lysis. We characterize lysis efficiency as a function of control parameters and demonstrate compatibility with enzymatic assays by measuring the catalysis of β-glucosidase, an important cellulase used in the conversion of biomass to biofuel. Our method enables assays in microfluidic droplets that are incompatible with detergents.     

Adding reagent to droplets with controlled rupture of encapsulated double emulsions

We present a method to add reagent to microfluidic droplets by enveloping them as a double emulsions in reagent-filled droplets and then rupturing them with an electric field. When the double emulsions rupture, they release their contents into the enveloping droplets, ensuring mixing with reagent while limiting cross-droplet contamination.     

Web-Scale Discovery in an Academic Health Sciences Library: Development and Implementation of the EBSCO Discovery Service

Funds made available at the close of the 2010–11 fiscal year allowed purchase of the EBSCO Discovery Service (EDS) for a year-long trial. The appeal of this web-scale discovery product that offers a Google-like interface to library resources was counter-balanced by concerns about quality of search results in an academic health science setting and the challenge of configuring an interface that serves the needs of a diverse group of library users. After initial configuration, usability testing with library users revealed the need for further work before general release. Of greatest concern were continuing issues with the relevance of items retrieved, appropriateness of system-supplied facet terms, and user difficulties with navigating the interface. EBSCO has worked with the library to better understand and identify problems and solutions. External roll-out to users occurred in June 2012.     

A Womb With a Political View: Barbara Park’s MA! There’s Nothing to Do Here!, Prenatal Parenting, and the Battle over Personhood

This essay makes the case that Barbara Park’s picture book MA! There’s Nothing to Do Here!: A Word from Your Baby-in-Waiting (2008) adds another equal-parts absurdist and alarming item to the ever-growing responsibilities of expecting mothers: ensuring that their fetus is entertained. The messages that Park’s narrative sends about fetal needs, however, have implications that extend beyond individual mothers; they also encompass larger societal issues concerning the origins of human life, the embryonic capacity for discomfort, and the existence of “fetal children.” Given the way in which MA! There’s Nothing to Do Here! ascribes thoughts and emotions to an unborn baby, the narrative engages with current highly-politicized questions about the rights of the unborn and the debate over personhood. The fetus in Park’s text may be ostensibly complaining about being bored in the womb, but he is making a far more powerful, if tacit, argument for life beginning before birth.     

Learner and instructor identified success factors in distance education

To better understand their perceptions of optimal tools and strategies for success, this research analyzed the experiences of learners and instructors in an online distance education environment. A qualitative constant comparative analysis methodology supported by an appropriate conceptual framework guided the study. Data were collected over multiple years and from multiple stakeholders. The study identified the following significant conclusions: the availability of multiple tools added flexibility to the learning environment; technology tools should appeal to multiple learning styles; collaboration, reflection, and building a learning community were important strategies supported by multiple tools; and participant satisfaction, appropriate prerequisite skills, and faculty and administrative involvement ensured programmatic success. According to this study, optimal distance education environments should address factors identified in the conceptual framework.