扁藻细胞电镜观察的制样技术

以海洋单细胞微藻亚心型扁藻为材料 ,对海水水样中活体单细胞微藻的亚显微结构观察样品的制备技术进行了探索。选用 2 .5 %的戊二醛固定液和 1 %的锇酸固定液对样品进行双固定的技术 ;先酒精后丙酮按浓度梯度顺次进行脱水 ;再用Epon81 2环氧树脂作为包埋剂进行包埋 ;最后经过醋酸铀 -枸橼酸铅双染色后将切片用透射电镜进行亚显微结构的形态观察。采用本方法进行水样中活体样品的电镜制片 ,拍得清晰度较高的电镜照片 ,并通过照片对水样中运动细胞透射电镜样品制备技术进行了分析。     

Pathogenic Vibrio spp. in the Sea Bream, Sparus sarba

VibriosisisacommonandseriousdiseasecausedbybacterialspeciesofthegenusVibrioinalargenumberofmarinefishes.Thediseaseischaracterisedbygeneralsepticaemia,superficialskinlesionsandextensivehaemorrhages[1].Upuntilaround1980,VibrioanguillarumwastheonlyVibriospecieswhichhadbeenshowntobethecausativeagentofvibriosis.However,recentstudieshaverevealedthatseveralotherVibriospeciessuchasV.alginolyticus,V.ordalli,V.damsela,andV.salmonicchwerealsoPOtentialfishpathogens.V.alginolyticus,V.porahaemolyticusa…     

挑选运动员在最合适的运动项目上

在人体中至少有七个遗传的因素影响着运动员的运动能力如:身高、体重、四肢长度、肌肉长度、肌腱附着点,肌纤维类型以及脂肪的百分比。这些遗传因素中的每一个因素与运动成绩的联系都很密切。身高身高常常是选择运动项目的一个主要决定因素。例如,大多数成功的篮球、排球和美式足球运动员其身高都是在人的平均身高之上;相反,在体操、跳水和摔跤项目上的许多运动员却是处在平均身高之下。通常在田径项目中,大多数跳高运动员的身材是比较高的,而大多数长跑运动员则相对较矮。     

吹起口哨——迪斯尼动画片《木偶奇遇记》插曲

当你有时身陷困境,是非分不{矿甲广.万.1卜.、___.一、二-.、、.,二l}j布衬『1.户口洲尸洲!一口口寻口尸.‘户)1旦,1于葫内7沁尸吞「「】!一厂户户.11尸派」」」」ll林飞尹’尸l叹…!.一1.’.一卫l厄二一口.一t夕.认i、」」」」ll二、」目l,_.l、l…ll五甘『i曰夕--一口!一口.‘日口--一【曰一函厄二ll宁葫,71尸丘莽马一!一声户-.一门叫一卜(.1」1l飞叭7『闷叹.尸,一衍「叹‘「{二l可州口--可厂。卜 1}日卜厂 }_:队,‘异一,以.‘、.}_.、气.卫!{洲公帐叼画卜罗闷、i1.习二i口.留枷泥ll‘气,时‘尸一i认尸户ll声一衬找不吞’气11{i压万.一l几…     

Reconciling rising serials costs, the serials budget, and reference needs in a medical library serials retrenchment program: a methodology

Devising a coherent serials retrenchment plan while maintaining quality reference service is a dilemma faced by increasing numbers of medical librarians. In 1991, the staff of the Shiffman Medical Library, Wayne State University, began to address increasing serials budget reductions that by 1994 amounted to a projected 20% maximum cut. The resulting retrenchment plan combined an ongoing serials use study, faculty and librarian reviews of cancellation lists, and systematic refinements in interlibrary cooperation and document delivery service. The Shiffman plan provides a constructive framework that can be adapted to other medical libraries facing immediate, major serials retrenchment.