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We have studied the contraction and extension of Vorticella convallaria and its mechanical properties with a microfluidic loading system. Cells of V. convallaria were injected to a microfluidic channel (500 μm in width and 100 μm in height) and loaded by flow up to ∼350 mm s−1. The flow produced a drag force on the order of nanonewton on a typical vorticellid cell body. We gradually increased the loading force on the same V. convallaria specimen and examined its mechanical property and stalk motion of V. convallaria. With greater drag forces, the contraction distance linearly decreased; the contracted length was close to around 90% of the stretched length. We estimated the drag force on Vorticella in the channel by calculating the force on a sphere in a linear shear flow. 相似文献
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Kazuya Takeda Soichiro Koyama Tomoko Nagai Hiroaki Sakurai Yoshikiyo Kanada 《Measurement in physical education and exercise science》2018,22(1):19-24
The aim of this study was to clarify the intra- and inter-rater reliability of the rate of force development in hip abductor muscle force measurements using a hand-held dynamometer. Thirty healthy adults were separately assessed by two independent raters on two separate days. Rate of force development was calculated from the slope of the force-time curve that was divided into four time intervals (50, 100, 200, and 300 ms). The highest values of the three trials were used for statistical analysis. The intra-class correlation coefficient with a 95% confidence interval, standard error of measurement, and minimal detectable change at the 95% confidence level were calculated for each interval. For assessment of systematic error, Bland-Altman analysis was used. The results indicated that intra- and inter-rater reliability of the highest values at all intervals were sufficient (intra-class correlation coefficient > .7). The Bland-Altman analysis did not show systematic error in either reliability measure. 相似文献
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Understanding the biological feeding strategy and characteristics of a microorganism as an actuator requires the detailed and quantitative measurement of flow velocity and flow rate induced by the microorganism. Although some velocimetry methods have been applied to examine the flow, the measured dimensions were limited to at most two-dimensional two-component measurements. Here we have developed a method to measure three-dimensional two-component flow velocity fields generated by the microorganism Vorticella picta using a piezoscanner and a confocal microscope. We obtained the two-component velocities of the flow field in a two-dimensional plane denoted as the XY plane, with an observation area of 455×341 μm2 and the resolution of 9.09 μm per each velocity vector by a confocal microparticle image velocimetry technique. The measurement of the flow field at each height took 37.5 ms, and it was repeated in 16 planes with a 2.50 μm separation in the Z direction. We reconstructed the three-dimensional two-component flow velocity field. From the reconstructed data, the flow velocity field [u(x,y,z),v(x,y,z)] in an arbitrary plane can be visualized. The flow rates through YZ and ZX planes were also calculated. During feeding, we examined a suction flow to the mouth of the Vorticella picta and measured it to be to 300 pl∕s. 相似文献
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Chikako Nagai 《Journal of Teaching in Social Work》2013,33(4):435-449
In general education, European American values stand as the unacknowledged norm and are perceived as being culturally neutral or culture free. By recognizing European American culture and spirituality as one of many diversities, social work students may better identify biased values and expectations inherent in the traditional monocultural and monospiritual approach. By providing space for European American culture and spirituality in social work education, among many others, intersubjective interactions become possible. While recognizing the increasing needs of multicultural/multispiritual heritage populations, in this article, the author reexamines cultural and spiritual competencies, focusing on relational issues that are not currently emphasized enough because of a strong conceptual association of competency with knowledge and skills-based practices. 相似文献
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Advisory boards typically offer guidance, support, social, and financial capital to academic units within colleges and universities. They are generally comprised of prominent volunteers from the community and appropriate industries or businesses. The results of this exploratory study found that non-alumni advisory board volunteers developed emotional connections and pride in serving the institution similar to alumni. The connections led most of the volunteers who participated in this study to have a high level of engagement with the institution that was meaningful to them and resulted in their desire to continue as volunteers, even without having earned a degree from the institution. 相似文献