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1.
Serap ?uhadar Mehmet K?seo?lu Yasemin ?inpolat Güler Bu?dayc? Murat Usta Tuna Semerci 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2016,26(1):53-60
Introduction
Extremely high glucose concentrations have been shown to interfere with creatinine assays especially with Jaffe method in peritoneal dialysate. Because diabetes is the fastest growing chronic disease in the world, laboratories study with varying glucose concentrations. We investigated whether different levels of glucose spiked in serum interfere with 21 routine chemistry and thyroid assays at glucose concentrations between 17-51 mmol/L.Materials and methods
Baseline (group I) serum pool with glucose concentration of 5.55 (5.44-5.61) mmol/L was prepared from patient sera. Spiking with 20% dextrose solution, sample groups were obtained with glucose concentrations: 17.09, 34.52, and 50.95 mmol/L (group II, III, IV, respectively). Total of 21 biochemistry analytes and thyroid tests were studied on Abbott c8000 and i2000sr with commercial reagents. Bias from baseline value was checked statistically and clinically.Results
Creatinine increased significantly by 8.74%, 31.66%, 55.31% at groups II, III, IV, respectively with P values of < 0.001. At the median glucose concentration of 50.95 mmol/L, calcium, albumin, chloride and FT4 biased significantly clinically (-0.85%, 1.63%, 0.65%, 7.4% with P values 0.138, 0.214, 0.004, < 0.001, respectively). Remaining assays were free of interference.Conclusion
Among the numerous biochemical parameters studied, only a few parameters are affected by dramatically increased glucose concentration. The creatinine measurements obtained in human sera with the Jaffe alkaline method at high glucose concentrations should be interpreted with caution. Other tests that were affected with extremely high glucose concentrations were calcium, albumin, chloride and FT4, hence results should be taken into consideration in patients with poor diabetic control.Key words: assay interference, glucose interference, preanalytical phase, creatinine, Jaffe kinetic assay, thyroid function tests 相似文献2.
Nam Cao Hoai Le Murat Gel Yonggang Zhu Jian Wang Helen Dacres Alisha Anderson Stephen C. Trowell 《Biomicrofluidics》2014,8(6)
Bioluminescence resonance energy transfer (BRET) is a form of Förster resonance energy transfer. BRET has been shown to support lower limits of detection than fluorescence resonance energy transfer (FRET) but, unlike FRET, has not been widely implemented on microfluidic devices for bioanalytical sensing. We recently reported a microscope-based microfluidic system for BRET-based biosensing, using a hybrid, high quantum-efficiency, form of BRET chemistry. This paper reports the first optical fiber-based system for BRET detection on a microfluidic chip, capable of quantifying photon emissions from the low quantum-efficiency BRET2 system. We investigated the effects of varying core diameter and numerical aperture of optical fibers, as well as varying microfluidic channel design and measurement conditions. We optimized the set-up in order to maximize photon counts and minimize the response time. The optimized conditions supported measurement of thrombin activity, with a limit of detection of 20 pM, which is lower than the microscope-based system and more than 20 times lower than concentrations reported to occur in plasma clots. 相似文献
3.
We present new methods of query expansion using terms that form lexical cohesive links between the contexts of distinct query terms in documents (i.e., words surrounding the query terms in text). The link-forming terms (link-terms) and short snippets of text surrounding them are evaluated in both interactive and automatic query expansion (QE). We explore the effectiveness of snippets in providing context in interactive query expansion, compare query expansion from snippets vs. whole documents, and query expansion following snippet selection vs. full document relevance judgements. The evaluation, conducted on the HARD track data of TREC 2005, suggests that there are considerable advantages in using link-terms and their surrounding short text snippets in QE compared to terms selected from full-texts of documents. 相似文献
4.
Bugholes are surface imperfections that appear as small pits and craters on concrete surface after the casting process. Bugholes are cosmetic surface imperfections and do not affect the structural integrity of the concrete structure. However, existences of these imperfections increase cost since bugholes require additional surface preparations before painting or finishing the concrete surface. Additionally, these surface imperfections cause secondary problems by decreasing the cover on RC making incretion of salts into the reinforcement bars inside RC. In this paper, authors discuss development of a new device to measure “bughole” rating of concrete surface. The innovative technique used in design enables us to manufacture a compact instrument, which is physically small, lightweight and practical. The paper explains the design principles of the device and the procedure used for optimization of design parameters for the best performance. Last section of the paper gives simulation results to compare the performance of the device to current state of the art methods used for bughole rating in industry. 相似文献
5.
Jyoti P. Gurung Moein Navvab Kashani Sanaz Agarwal Gonzalo Peralta Murat Gel Matthew A. B. Baker 《Biomicrofluidics》2021,15(3)
Many motile bacteria are propelled by the rotation of flagellar filaments. This rotation is driven by a membrane protein known as the stator-complex, which drives the rotor of the bacterial flagellar motor. Torque generation is powered in most cases by proton transit through membrane protein complexes known as stators, with the next most common ionic power source being sodium. Sodium-powered stators can be studied through the use of synthetic chimeric stators that combine parts of sodium- and proton-powered stator proteins. The most well studied example is the use of the sodium-powered PomA-PotB chimeric stator unit in the naturally proton-powered Escherichia coli. Here we designed a fluidics system at low cost for rapid prototyping to separate motile and non-motile populations of bacteria while varying the ionic composition of the media and thus the sodium-motive force available to drive this chimeric flagellar motor. We measured separation efficiencies at varying ionic concentrations and confirmed using fluorescence that our device delivered eightfold enrichment of the motile proportion of a mixed population. Furthermore, our results showed that we could select bacteria from reservoirs where sodium was not initially present. Overall, this technique can be used to implement the selection of highly motile fractions from mixed liquid cultures, with applications in directed evolution to investigate the adaptation of motility in bacterial ecosystems. 相似文献
6.
Çakır Nur Akkuş Çakır Murat Perit Lee Frank J. 《Educational technology research and development : ETR & D》2021,69(5):2683-2703
Educational technology research and development - This paper presents a game-design workshop built around a digital art installation featuring video games displayed over a real-world skyscraper to... 相似文献
7.
Colin Latchem Insung Jung Kumiko Aoki Ali Ekrem Ozkul 《British journal of educational technology : journal of the Council for Educational Technology》2008,39(4):610-630
Japan and Korea have highly developed information and communications technology infrastructures and have recently reformed their higher education systems and encouraged e-transformation. However, Japanese universities have not embraced e-learning as wholeheartedly as their Korean counterparts. The paper concludes that this is due to governmental and institutional failure to develop a cogent vision and strategic plan for e-learning, to recognise the need for fundamental shifts in organisational cultures and structures and pedagogy and to provide training, funding, recognition and reward for such development. It attributes this to differences in the cultural perspectives, values and customs of the two countries' policy makers, managers and faculty members. The findings are seen to hold important lessons for other countries embarking on e-transformation. 相似文献
8.
Brian Hand Lori A. Norton-Meier Murat Gunel Recai Akkus 《International Journal of Science and Mathematics Education》2016,14(5):847-863
How can classrooms become communities of inquiry that connect intellectually challenging science content with language-based activities (opportunities to talk, listen, read, and write) especially in settings with diverse populations? This question guided a 3-year mixed-methods research study using the Science Writing Heuristic (SWH) approach in cooperation with 2 universities, area education agencies, 6 school districts, 32 elementary teachers, and over 700 students each year. The participating teachers engaged in a yearly summer institute, planned units, implemented this curriculum in the classroom, and contributed to ongoing data collection and analysis. Findings demonstrate that critical embedded language opportunities contribute to an increase in student Iowa Tests of Basic Skills (ITBS) scores in science and language based on level of implementation particularly for elementary students who receive free and reduced lunch (an indicator of living at the poverty level). 相似文献
9.
Alipa?a Ayas Haluk ?zmen Muammer ?alik 《International Journal of Science and Mathematics Education》2010,8(1):165-184
The aim of the present study is to elicit students’ understanding of the particulate nature of matter via a cross-age study
ranging from secondary to tertiary educational levels. A questionnaire with five-item open-ended questions was administered
to 166 students from the secondary to tertiary levels of education. In light of the findings, it can be deduced that the number
of students’ responses categorized under the “sound understanding” category for each item increased with educational level,
except for U1. Also, it can be concluded that students’ specific misconceptions decreased steadily from SHS1 to SHS3, except
for item 4, but there is surprisingly a clear increase at U1. 相似文献
10.
Murat Bursal 《International Journal of Science and Mathematics Education》2010,8(4):649-666
This study investigated Turkish preservice, elementary teachers’ personal mathematics teaching efficacy (PMTE), and science
teaching efficacy (PSTE) beliefs at the end of their teacher education program. A majority of the participants believed they
were well prepared to teach both elementary mathematics and science, but their PSTE scores were significantly lower than their
PMTE scores. However, a significant correlation was found between the PMTE and PSTE scores. No significant gender effect on
PMTE and PSTE scores was observed, but unlike the results from other countries, Turkish female preservice elementary teachers
were found to have slightly higher PMTE and PSTE scores than their male peers. High school major area was found to be a significant
predictor of participants’ PMTE and PSTE scores. Participants with mathematics/science high school majors were found to have
significantly higher PMTE and PSTE scores than those with other high school majors. 相似文献