Muscle metabolism and performance improvement after two training programmes of sprint running differing in rest interval duration

Repeated-sprint training often involves short sprints separated by inadequate recovery intervals. The effects of interval duration on metabolic and performance parameters are unclear. We compared the effects of two training programmes, differing in rest interval duration, on muscle (vastus lateralis) metabolism and sprint performance. Sixteen men trained three times a week for 8 weeks, each training session comprising 2-3 sets of two 80-m sprints. Sprints were separated by 10 s (n = 8) or 1 min (n = 8). Both training programmes improved performance in the 100-, 200-, and 300-m sprints, but the improvement was greater in the 10-s group during the final 100 m of the 200- and 300-m runs. Independent of interval duration, training mitigated the drop of muscle ATP after two 80-m sprints. The drop in phosphocreatine and the increases in glucose-6-phosphate and fructose-6-phosphate after two 80-m sprints were greater in the 10-s group. In conclusion, training with a limited number of repeated short sprints (≤10 s) may be more effective in improving speed maintenance in 200- and 300-m runs when performed with a 1:1 rather than a 1:6 exercise-to-rest ratio. This may be due to a greater activation of glycolysis caused, in part, by the limited resynthesis of phosphocreatine during the very short rest interval.     

Accuracy of a Perspective Control Lens

Abstract

The contextual interference (CI) effect has been replicated many times since its first demonstration by Shea and Morgan (1979) in the motor learning domain (see Brady, 1998; Magill&;Hall, 1990). The CI effect is characterized by the observation that experiencing greater interference during acquisition is detrimental to immediate performance but enhances delayed performance as measured on retention or transfer tests. High CI is most often created by random practice in which the learning of multiple tasks occurs in a single training. In contrast, low CI is frequently created by using a blocked practice format in which all the practice trials of one task are completed before another task is introduced. One theoretical account that has been forwarded to account for the CI effect is labeled the action plan reconstruction hypothesis (Lee&;Magill, 1983, 1985). This position intimates that before a movement occurs an “action plan” must be prepared. In blocked practice, a previously prepared “action plan” is readily available from trial to trial, but it suffers from lack of attention on trials following initial retrieval from working memory. In random practice, however, each time a task must be executed a “reconstruction” of the action plan must be processed, because the interchange of information from trial to trial never allows the same information to remain in working memory for an extended amount of time. Presumably, the additional trial-to-trial preparation used by the random practice participant during practice results in a more resilient memory representation that better supports long-term recall efforts compared to their blocked practice counterparts.     

Gateway finder in large graphs: problem definitions and fast solutions

Given a graph, how to find a small group of ‘gateways’, that is a small subset of nodes that are crucial in connecting the source to the target? For instance, given a social network, who is the best person to introduce you to, say, Chris Ferguson, the poker champion? Or, given a network of people and skills, who is the best person to help you learn about, say, wavelets? We formally formulate this problem in two scenarios: Pair-Gateway and Group-Gateway. For each scenario, we show that it is sub-modular and thus it can be solved near-optimally. We further give fast, scalable algorithms to find such gateways. Extensive experimental evaluations on real data sets demonstrate the effectiveness and efficiency of the proposed methods.     

Technique and timing in the women's reverse two and one half somersault tuck (305C) and the men's reverse two and one half somersault pike (305B) 3m springboard dives

The purpose of this study was to compare the reverse two and one half somersault dive in a tuck position (305C) performed by females (n = 24), and the reverse two and one half somersault dive in a pike position (305B) performed by males (n = 21), to determine changes required by females to successfully perform 305B. Key performance variables in reverse dives were also compared to those of forward dives. Video data of the dives performed at the 1999 FINA World Diving Cup were captured and digitised to obtain times and postures of the divers at specific events including hurdle landing, takeoff, and entry. Estimates of hurdle flight height and mass-normalised work done on the springboard were obtained from hurdle and flight times. The males did more work on the springboard to achieve greater height and rotation than females. Females performing 305C had less hip and knee flexion at hurdle landing than males performing 305B and took longer to achieve maximum hip flexion after takeoff from the springboard. To progress from 305C to 305B females need to adjust their techniques to put more energy into the system. Desirable changes include increased height in the hurdle and increased hip and knee flexion prior to hurdle landing. Comparison of results for reverse dives with data previously presented for forward dives indicated that divers are more limited in the number of somersaults and dive position in reverse dives than forward dives despite equivalent or better height in reverse dives than forward dives.     

Enhanced production of thermostable laccases from a native strain of Pycnoporus sanguineus using central composite design

研究目的：优化获得血红密孔菌（P.sanguineus）的最佳培养基组成,提高耐热漆酶的产量。创新要点：获得了目前文献报道的最高水平的漆酶活力。研究方法：通过单因素试验研究了不同培养基（番茄汁、麦麸、麦芽提取物和葡萄糖细菌蛋白胨培养基）和不同组合诱导剂（大豆油、阿魏酸、没食子酸、二甲基苯胺、酸性蓝62和活性蓝19分别与硫酸铜组合诱导剂）对P.sanguineus产耐热漆酶的影响。在此基础上采用中心组合试验设计,进一步研究了番茄汁培养基结合硫酸铜和大豆油组合诱导剂对P.sanguineus产耐热漆酶的影响。利用SAS10.0和响应面分析方法对试验结果进行了统计分析和建立回归模型。重要结论：通过中心组合设计优化得出P.sanguineus产耐热漆酶的最优培养基条件：以36.8%番茄汁为培养基,以3 mmol/L硫酸铜和1%大豆油作为组合诱导剂。该条件下在10 L搅拌槽生物反应器中漆酶活力达到了143 000 IU/L（2,2＇-联氮双（3-乙基苯并噻唑啉-6-磺酸）为底物,pH值为3.0）。     

Paper-based colorimetric enzyme linked immunosorbent assay fabricated by laser induced forward transfer

We report the Laser Induced Forward Transfer (LIFT) of antibodies from a liquid donor film onto paper receivers for application as point-of-care diagnostic sensors. To minimise the loss of functionality of the active biomolecules during transfer, a dynamic release layer was employed to shield the biomaterial from direct exposure to the pulsed laser source. Cellulose paper was chosen as the ideal receiver because of its inherent bio-compatibility, liquid transport properties, wide availability and low cost, all of which make it an efficient and suitable platform for point-of-care diagnostic sensors. Both enzyme-tagged and untagged IgG antibodies were LIFT-printed and their functionality was confirmed via a colorimetric enzyme-linked immunosorbent assay. Localisation of the printed antibodies was exhibited, which can allow the creation of complex 2-d patterns such as QR codes or letters for use in a final working device. Finally, a calibration curve was determined that related the intensity of the colour obtained to the concentration of active antibodies to enable quantitative assessment of the device performance. The motivation for this work was to implement a laser-based procedure for manufacturing low-cost, point-of-care diagnostic devices on paper.     

A Web adaptive educational system for people with hearing difficulties

In this paper we present a web adaptive educational tool for the Greek Sign Language. The design and the implementation of the tool were partially funded by national bodies. The paper presents the architecture of the tool, in an abstract way and briefly describes its functionality. The interaction process followed is also presented.     

Diving

The purpose of this study was to compare the reverse two and one half somersault dive in a tuck position (305C) performed by females (n = 24), and the reverse two and one half somersault dive in a pike position (305B) performed by males (n = 21), to determine changes required by females to successfully perform 305B. Key performance variables in reverse dives were also compared to those of forward dives. Video data of the dives performed at the 1999 FINA World Diving Cup were captured and digitised to obtain times and postures of the divers at specific events including hurdle landing, takeoff, and entry. Estimates of hurdle flight height and mass‐normalised work done on the springboard were obtained from hurdle and flight times. The males did more work on the springboard to achieve greater height and rotation than females. Females performing 305C had less hip and knee flexion at hurdle landing than males performing 305B and took longer to achieve maximum hip flexion after takeoff from the springboard. To progress from 305C to 305B females need to adjust their techniques to put more energy into the system. Desirable changes include increased height in the hurdle and increased hip and knee flexion prior to hurdle landing. Comparison of results for reverse dives with data previously presented for forward dives indicated that divers are more limited in the number of somersaults and dive position in reverse dives than forward dives despite equivalent or better height in reverse dives than forward dives.