Spatially gradated segregation and recovery of circulating tumor cells from peripheral blood of cancer patients

For cancer patients, the enumeration of rare circulating tumor cells (CTCs) in peripheral blood is a strong prognostic indicator of the severity of the cancer; for the general population, the capture of CTCs is needed for use as a clinical tool for cancer screening, early detection, and treatment assessment. Here, we present a fast, high-purity (∼90%) and high-efficiency (>90%) method for the segregation and undamaged recovery of CTCs using a spatially gradated microfluidic chip. Further, by lysing the red blood cells we achieved not only a significant reduction in the overall processing time but also mitigated the blood clogging problem commonly encountered in microfluidic-based CTC isolation systems. To clinically validate the chip, we employed it to detect and capture CTCs from 10 liver cancer patients. Positive CTC enumeration was observed in all the blood samples, and the readings ranged from a low of 1–2 CTCs (1 patient) to a high of >20 CTCs (2 patients) with the balance having 3–20 CTCs per 3-ml blood sample. The work here indicates that our system can be developed for use in cancer screening, metastatic assessment, and chemotherapeutic response and for pharmacological and genetic evaluation of single CTCs.     

培养健康的企业文化

企业文化是什么?是企业的精神,还是企业的理念?美国的约翰·P·科特和詹姆斯·L·赫斯科特认为,企业文化是一个企业中各个部门,至少是高层管理者们所共同拥有的那些企业价值观念和经营实践,指企业中各个职能部门或地处于不同地理环境的部门所拥有的那种共同的文化现象;特雷斯·E·迪尔和阿伦·A·肯尼迪认为企业文化是价值观、英雄人物、习俗仪式、文化网络、企业环境。     

基于人口普查与多源夜间灯光数据的海岸带人口空间化分析

我国海岸带区域是台风、风暴潮、地震海啸和海岸侵蚀等海洋灾害的重灾区,精细空间分辨率的人口数据,能够有效服务海岸带灾害风险管理。本文基于我国第六次人口普查资料、OLS/DMSP 和NPP/VIIRS DNB 两种夜间灯光数据及Landsat卫星遥感影像,综合利用遥感与地理信息系统理论与技术,进行了我国海岸带人口空间化方法与应用研究。利用建筑物与裸地增强指数法（EBBI）基于Landsat 卫星遥感影像提取了我国沿海区县建成区数据,作为人口分布的空间控制因素,以普查人口数与夜间灯光数据回归函数关系为依据,对人口进行空间化处理。反演得到了我国海岸带区县1km×1km和0.5km×0.5km两个空间尺度的人口格网数据。并利用福建省沿海乡镇人口数据对人口空间化结果进行了精度评价。研究结果表明NPP/VIIRS DNB夜间灯光数据适用于人口空间化研究,且其反演精度整体优于基于DMSP/OLS传统夜间灯光数据反演的人口格网模型。通过本文实践,可以发现NPP/VIIRS DNB夜间灯光数据具有实现人口和社会经济数据空间化的巨大潜力。     

加入WTO后成人高等教育面临的新形势、挑战与发展对策探讨

面对“入世”新的经济形势，成人高等教育面临新的挑战和发展机遇，必须树立新的教育思想观念，大力发展远程网络教育，大力发展高层次继续教育，为社会培养更多具有创新能力的有用人才。     

A Supervisory Framework for Systematically Attending to Outcomes in Clinical Supervision

Examination of the efficacy of clinical supervision has accrued limited evidence, partially due to lack of a clarity or consensus on what constitutes supervision outcomes. This not only leads to inconsistency across studies evaluating supervision outcomes but also falls short of providing guidance for clinical supervisors to systematically evaluate the efficacy of their work. We present a practitioner-oriented, evidence-informed, and pan-theoretical framework that connects a broad range of outcomes to the supervision process, including proximal, intermediate, and distal outcomes. We discuss how several practitioner-friendly instruments related to these outcomes can be incorporated into supervision practice and contribute to a more holistic assessment of supervision outcomes. We also discussed supervisory strategies, such as strategically attending to and balancing multiple levels of outcomes and incorporating a theoretical lens to our framework.

     

Counselors’ challenging experience in integrated behavioral healthcare: A qualitative exploration

Using a consensual qualitative research method, we interviewed 16 counselors on the challenges that they experienced when working in integrated behavioral healthcare settings. We identified five domains and 13 core ideas among all domains. We also discussed the implications for future counselor training and research.     

微气候在景观设计中的应用前景探究

地形、建筑、水体、植物是园林景观的四大构成要素,其中植物是影响园林景观整体效果的最主要因素,同时,微气候学中提到,植物对有利的微气候的创造具有积极作用,故而微气候学与景观设计是两门密不可分的学科。微气候营造在小尺度园林规划设计中越来越受到重视,目的是构建舒适宜人的环境。微气候的营造在未来景观设计中将成为一种最重要的设计手段和评价标准。     

A Grounded Theory Analysis of Cultural Humility in Counseling and Counselor Education

Using a grounded‐theory methodology, we interviewed 14 experienced counselor educators regarding the enactment and development of cultural humility (CH). We present a coherent theory depicting a cyclical process of CH involving lifelong learning. Implications include enacting CH in addressing microaggressions in the therapeutic setting and incorporating critical incident reflection in CH in counselor education.     

国外知识转移研究的若干问题

对知识转移的定义、知识转移的过程、知识转移的关键影响因素进行总结和归纳,对目前国内学者研究较少的知识转移速度问题,也进行阐述,在整理文献的基础上,提出知识转移的横向知识转移和纵向知识转移。