排序方式: 共有14条查询结果,搜索用时 31 毫秒
1.
Samir P. Patel Subhash D. Katewa Surendra S. Katyare 《Indian journal of clinical biochemistry : IJCB》2005,20(1):1-8
Effects of treatmentin vivo with the antimalarials:chloroquine (CQ), primaquine (PQ) and quinine(Q) on lysosomal enzymes and lysosomal membrane integrity
were examined. Treatment with the three antimalarials showed an apparent increase in the membrane stability. CQ treatment
resulted in increase in both the ‘free’ and ‘total’ activities of all the enzymes i.e. acid phosphatase, RNase II, DNase II
and cathepsin D. PQ treatment lowered the ‘free’ and ‘total’ activities of acid phosphatase and cathepsin D, but the DNase
II activities increased. Treatment with Q resulted in increased ‘free’ and ‘total’ activities of RNase II and DNase II. While
‘free’ activities of acid phosphatase and cathepsin D were low; the ‘total’ activities increased significantly. Our results
suggest that a generalized increase in free nucleases activities following prolonged treatment with antimalarials may lead
to cell damage and/or necrosis. 相似文献
2.
Putative Role of Cardio Metabolic Risk Among Poorly Controlled Asthmatics in South Indian Population
Murugaiyan Sathish Babu K. P. Sreesoorya Surendra K. Menon Subiman Saha A. R. Srinivasan S. Arul Vijaya Vani R. Reeta V. Kuzhandai Velu 《Indian journal of clinical biochemistry : IJCB》2017,32(2):225-229
Mortality and morbidity attributed to asthma remains to be the biggest nightmare worldwide. Hence, the study was aimed to compare the cardio metabolic risk factors as assessed by Body mass index (BMI), waist hip ratio (WHR), serum triacylglycerol and uric acid in well controlled and poorly controlled asthmatics and to correlate these parameters with the severity of asthma. A case control study was conducted on 90 subjects who were segregated into well controlled asthmatics (n = 30) and poorly controlled asthmatics (n = 30) who were diagnosed based on Global initiative for Asthma management guidelines and healthy volunteers (n = 30). Centrifuged fasting venous blood samples were used for biochemical analysis, pulmonary function test, BMI, and waist hip ratio (WHR) were measured. The statistical analysis was done using SPSS version 17. There was a significant increase in BMI, WHR, lipid profile, serum uric acid and decrease in forced expiratory volume (FEV1), forced vital capacity (FVC), and FEV1/FVC in poorly controlled asthmatics. There was a significant association between FEV1 and serum uric acid, BMI and Triacylgycerol in poorly controlled asthmatics. Poorly controlled asthmatics have greater risk of developing cardiometabolic problems. Serum uric acid can be used as one of the severity markers in asthma to assess cardio metabolic risk. 相似文献
3.
Aniket V. Mali Sunita S. Bhise Surendra S. Katyare Mahabaleshwar V. Hegde 《Indian journal of clinical biochemistry : IJCB》2018,33(1):38-45
Recent studies have been noted that the erythrocytes from Type II diabetic patients show significantly altered structural and functional characteristics along with the changed intracellular concentrations of glycolytic intermediates. More recent studies from our laboratory have shown that the activities of enzymes of glycolytic pathway changed significantly in RBCs from Type II diabetic patients. In particular the levels of lactate dehydrogenase (LDH) increased significantly. Lactic acidosis is an established feature of diabetes and LDH plays a crucial role in conversion of pyruvate to lactate and reportedly, the levels of lactate are significantly high which is consistent with our observation on increased levels of LDH. Owing to this background, we examined the role of erythrocyte LDH in lactic acidosis by studying its kinetics properties in Type II diabetic patients. Km, Vmax and apparent catalytic efficiency were determined using pyruvate and NADH as the substrates. With pyruvate as the substrate the Km values were comparable but Vmax increased significantly in the diabetic group. With NADH as the substrate the enzyme activity of the diabetic group resolved in two components as against a single component in the controls. The Apparent Kcat and Kcat/Km values for pyruvate increased in the diabetic group. The Ki for pyruvate increased by two fold for the enzyme from diabetic group with a marginal decrease in Ki for NADH. The observed changes in catalytic attributes are conducive to enable the enzyme to carry the reaction in forward direction towards conversion of pyruvate to lactate leading to lactic acidosis. 相似文献
4.
并行程序通常用来提高计算性能,但却受到I/O存取过程中的大延迟的影响。鉴于处理器与I/O之间差距越来越大,数据存取延迟成为主要的性能瓶颈。我们认为是时候重新审视“I/O墙”的问题并用额外的计算能力换取数据存取速度。我们提出一种新的用于掩盖I/O延迟的预执行策略。我们介绍了这种预执行的I/O预取框架,这种预取线程的构造方法,底层支持库, 相似文献
5.
Vidya Akhileshwar Samir P. Patel Surendra S. Katyare 《Indian journal of clinical biochemistry : IJCB》2007,22(1):84-90
Studies were carried out to examine and compare the effects of alloxan-diabetes on reactive oxygen species (ROS) related parameters
in the heart from male and female rats. Effects of insulin treatment were also evaluated. The diabetic state severely compromised
the ROS defense mechanism in the cardiac tissue and the effects were more pronounced in the female than in the male rats.
There was several fold increase in the xanthine oxidase (XO) activity in general and the magnitude of increase was higher
in the females; insulin treatment resulted in further increase in the XO activity. The glucose-6-phosphate dehydrogenase (G6PDH)
and catalase activities decreased and the reduced glutathione (GSH) content in mitochondria was completely depleted in diabetic
state with significant decrease in the GSH levels in the post-mitochondrial fraction; the effect was more pronounced in the
females. The superoxide dismutase (SOD) and glutathione peroxidase (GPox) activities increased in the diabetic state to a
greater extent in male rats. Insulin treatment had restorative action only on some parameters. In conclusion, our results
suggest that diabetic state may further compromise the weak ROS defense systems in the heart thus initiating a lesion at the
level of mitochondria which ultimately leads to cardiomyopathy and the effects are especially more pronounced in the females.
Our results also pointed out that insulin treatment was ineffective in restoring ROS related parameters. 相似文献
6.
Jagannath G. Satav Surendra S. Katyare 《Indian journal of clinical biochemistry : IJCB》2004,19(2):23-31
The reports in the literature on effects of diabetes on mitochondrial energy-linked functions are conflicting. Hence we carried
out systematic studies to evaluate the effects at the early and the late stages of the disease using STZ-diabetic rat as a
model. At the end of one week, after induction of diabetes, respiration rates with glutamate and succinate as the substrates
increased; respiration rates with other substrates e.g. β-hydroxybutyrate, pyruvate + malate and ascorbate + TMPD were not
affected despite substantial decrease in the β-hydroxybutyrate dehydrogenase activity and cytochrome b and c+c1 contents. Insulin treatment brought about increase in the cytochrome contents beyond control values. The ATPase activity
was generally low in the diabetic animals and was not restored by insulin treatment.
At the end of one month, the respiratory activities with all the substrates were generally low. Insulin treatment either restored
or stimulated the respiration rates beyond control values. The content of cytochromes was differentially affected in the diabetic
animals, but insulin treatment caused significant increase beyond control levels. The pattern for ATPase activity was similar
to the early effects.
At both the stages i.e. early and late stages of diabetes the mitochondria were tightly coupled. The ADP/O ratios were in
normal expected ranges and the respiratory control ratios were comparable with the control groups. Insulin treatment resulted
in apparent restoration of respiratory activity. However, the effects on the cytochromes and dehydrogenases activities were
differential. Taken together the two observations would suggest that the mitochondria were not re-instated to normality despite
apparent restoration of respiratory function. 相似文献
7.
Hiren R. Modi Surendra S. Katyare Samir P. Patel 《Indian journal of clinical biochemistry : IJCB》2008,23(3):272-278
Effects of thyroidectomy (Tx) and subsequent treatment with 3,5,3’-triiodothyronine (T3), and combined treatment (TR) with T3 + thyroxine (T4) on substrate kinetics properties of cytochrome oxidase of rat liver mitochondria were examined. Tx resulted in lowering of cytochromes content with decrease in the enzyme activity, and Km and Vmax. T3 and TR regimens restored the cytochromes contents and the Vmax values to normal. In control, T3 and TR groups the enzyme activity resolved in two kinetic components; in Tx group three kinetic components were evident. The Km values for all components decreased significantly in the experimental groups with concomitant increase in catalytic efficiency,
Kcat/Km. Significant alterations in the contents of total phospholipid and of cholesterol were noted while the changes in the phospholipids
composition were only of restricted nature. Regression analysis revealed that total phospholipid, cholesterol and phosphatidylcholine,
phosphatidylethanolamine play significant role in fine tuning the enzyme activity. 相似文献
8.
Rathore SS Agarwal SK Pande S Singh SK Mittal T Mittal B 《Indian journal of clinical biochemistry : IJCB》2011,26(3):222-229
Coumarinic oral-anticoagulants (COAs) are commonly used for treatment of thromboembolic events. However, these medications
have a narrow therapeutic range and there are large inter-individual variations in drug response. This is especially important
in the initial phases of oral-anticoagulant therapy. Recent advancements in pharmacogenetics have established that clinical
outcomes in oral-anticoagulant therapy are affected by genetic factors. The allelic variants of genes like cytochrome P450
2C9 (CYP2C9) and vitamin K epoxide reductase complex subunit 1 (VKORC1) are closely associated with maintenance dose of oral
anti-coagulants. In addition, GGCX (Gamma-glutamyl carboxylase) polymorphism at position 12970 (rs11676382), CYP4F2 (rs2108622;
V433M; 1347 C > T) and Apolipoprotein E (APOE) variants have been shown to explain a small but significant influence on dose
requirements. There are large differences in the frequencies of these polymorphisms between different world populations which
are also related to the requirements of oral anticoagulants. However, the final drug dosage in an individual is determined
by complex sets of genetic and environmental factors and several dosing algorithms which combine clinical and genetic parameters
to predict therapeutic COA doses have also been developed. The algorithm based dose prediction shows the importance of pharmacogenetic
testing in patients undergoing oral anticoagulant therapies. 相似文献
9.
Aniket V. Mali Sunita S. Bhise Mahabaleshwar V. Hegde Surendra S. Katyare 《Indian journal of clinical biochemistry : IJCB》2016,31(3):321-325
The activity of enzymes of glycolysis has been studied in erythrocytes from type-II diabetic patients in comparison with control. RBC lysate was the source of enzymes. In the diabetics the hexokinase (HK) activity increased 50 % while activities of phosphoglucoisomerase (PGI), phosphofructokinase (PFK) and aldolase (ALD) decreased by 37, 75 and 64 % respectively but were still several folds higher than that of HK. Hence, it is possible that in the diabetic erythrocytes the process of glycolysis could proceed in an unimpaired or in fact may be augmented due to increased levels of G6P. The lactate dehydrogenase (LDH) activity was comparatively high in both the groups; the diabetic group showed 85 % increase. In control group the HK, PFK and ALD activities showed strong positive correlation with blood sugar level while PGI activity did not show any correlation. In the diabetic group only PFK activity showed positive correlation. The LDH activity only in the control group showed positive correlation with marginal increase with increasing concentrations of glucose. 相似文献
10.
Reactive oxygen species (ROS) are believed to be responsible for pathogenesis of various diseases affecting tissues and systems.
ROS generated by mitochondrial electron transport chain as well as extra-mitochondrially are eliminated by the respective
defense mechanisms. We checked the activity of ROS generating system such as xanthine oxidase and also the parameter of ROS
defense mechanism e.g. superoxide dismutase (SOD), catalase, glutathione peroxidase (GPox), reduced glutathione content (GSH)
and glucose-6-phosphate dehydrogenase (G6PDH) in mitochondrial and post-mitochondrial fractions from various tissues (liver,
kidney, brain and heart) of normal rats. Extent of lipid peroxidation (LPO) which is immediate consequence of ROS generation
was also examined. Our results shows that significant tissue-specific differences exist in mitochondrial and cytosolic ROS
generating systems and ROS defense mechanisms. 相似文献