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1.
G. N. Raval L. J. Parekh D. D. Patel F. P. Jha R. N. Sainger P. S. Patel 《Indian journal of clinical biochemistry : IJCB》2004,19(2):60-71
Sialic acid, the end moieties of the carbohydrate chains are biologically important and essential for functions of glycoconjugates
and are reported to be altered in cancer patients. Two hundred and twenty five breast cancer (BC) patients, 100 patients with
benign breast disease (BBD) and 100 healthy females (controls) were enrolled for the study. Eight hundred and twenty four
follow-up samples of 225 breast carcinoma patients were also evaluated. The association of sialic acid forms, sialyltransferase
and α-2–6 sialoproteins levels with presence and extent as well as prognosis of breast carcinoma was studied. Serum sialic
acid forms and sialyltransferase revealed significantly elevated levels among untreated breast cancer patients as compared
to the controls, patients with BBD as well as cancer patients in remission. Non-responders showed comparable levels of the
markers with those found in breast cancer patients at the time of diagnosis. Higher levels of sialic acid forms at diagnosis
were associated with poor prognosis. A positive correlation between serum levels of different forms of sialic acids and extent
of malignant disease was observed. The changes in serum proteins with terminal α-2–6 sialic acid correlated well with alterations
in the levels of sialic acid forms and sialyltransferase. Malignant tissues showed elevated levels of sialic acid and sialyltransferase
as compared to surrounding normal tissues.
The results suggested potential utility of these markers in evaluation of clinical outcome. 相似文献
2.
Puja kumari Jha Nirupama Sharma Juhee Chandra Rachna Agarwal 《Indian journal of clinical biochemistry : IJCB》2021,36(3):337
Variability in analytical performance of some analyte indicated the need of evaluation of quality plan of our laboratory. We tried to put the same degree of effort into our quality metrics as we put into the laboratory processes themselves. Application of six sigma methodologies improve the quality by focusing on the root causes of the problems in performance and analyzing by flowcharts, fishbone diagrams and other quality tools. Sigma metric was calculated for laboratory parameters for a period of 8 months during 2018–19. The analytes with poor sigma metric were free Thyroxine (FT3, FT4), Sodium, Calcium and Magnesium. Sigma metric of free Thyroxine (FT3, FT4), Sodium, Calcium and Magnesium were below 3. A road map for process improvement was designed with DMAIC (Define-Measure-Analyze-Improve-Control) model to solve the issue. Possible causes for low analytical performance of the particular analytes were depicted in Fishbone diagram. The Fishbone analysis identified the water quality issues with electrolyte analysis while high ambient temperature was culprit for poor assay performance of free Thyroxine. Sigma metric of the analytical performance was assessed once again after root cause analysis. Sigmametric showed marked improvement in control phase. Identification of problems led to reduction in non value added work leading to adequate resource utilization by addressing the priority issue. Therefore, DMAIC tool with Fish bone model analysis can be recommended as a well suited method for troubleshooting in poor performance of laboratory parameter. 相似文献
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Sonika Gupta Niraj Shende Swati Banerjee Satish Kumar M. V. R. Reddy Bhaskar C. Harinath 《Indian journal of clinical biochemistry : IJCB》2002,17(1):5-8
Tuberculosis remains major health problem in India and developing countries Immunodiagnosis has important role in screening,
diagnosis and management of tuberculosis. SEVA TB ES-31 antigen has shown potential in detecting tuberculous IgG antibody
in earlier studies from our laboratory. In the present study we have analysedSEVA TB ES-31 antigen specific immunoglobulinsIgM, IgA and IgG in clinically and bacteriologically confirmed pulmonary tuberculosis cases to determine the usefulness of specific immunoglobulin
class in the diagnosis of patients attending the hospital.
Of the 30 cases of pulmonary tuberculosis 25 (83.3%) were positive for IgG, 19 (63.3%) for IgM and 16 (53.3%) for IgA. On
combining IgG and IgM positivity, sensitivity was increased to 93.3%. While combining IgG and IgA positivity, sensitivity
increased to 90%. However specificity was decreased to 66.6% and 70% for both of these combinations respectively. It could
be envisaged from this study that IgG antibody detection against ES-31 antigen showed acceptable sensitivity (83.3%) and specificity
(86.6%) compared to IgM or IgA alone or in combination. When immune responses were analysed according to degree of sputum
positivity, IgG response was observed to be predominant in all grades, compared to IgM or IgA antibody. The addition of IgM
or IgA as an adjunct test increases the sensitivity but at the cost of specificity. Hence the detection of IgG alone is more
useful compared to IgM or IgA assay, in detecting tuberculosis disease cases coming to the hospital. 相似文献
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Pragati Priyadarshinee Rakesh D. Raut Manoj Kumar Jha Sachin S. Kamble 《The Journal of High Technology Management Research》2017,28(2):221-245
The purpose of this research article is to identify the critical success criteria/factors that affect the cloud computing adoption and examine structure, reliability and validity of the criteria in the SMEs industry. This study considers nine types of criteria/factors with fifty-one sub-criteria, which have somewhat previously been used, and creates a ranking model that offers decision makers to measure the prior implementation of cloud computing adoption. A questionnaire-based survey research was used to collect data from 110 firms belonging to the SMEs industry in India. In this paper, the data collected was analysed by an integrated approach. Firstly, an Analytic Hierarchy Process approach was applied to find the significant fact of each criterion as the assessable indices of the cloud computing adoption. Secondly, an Exploratory Factor Analysis and Confirmatory Factor Analysis were used to examine structure, reliability and validity of the criteria. The data was tabulated in a Microsoft Excel sheet and then imported in SPSS (Statistical Package for the Social Sciences version 20) for analysis. The findings discovered that ‘Security and Privacy’, ‘Organizational Risk’, ‘Sharing and Collaboration’, ‘Confidentiality’, and ‘Integrity’ have a major effect on the adoption of cloud computing. The research was conducted in the SMEs industry in India, which may limit the generalizability of the findings. The findings and recommendations offer cloud computing service providers as well as users with a better understanding of what affects the cloud computing adoption feature, with a relevant understanding of the current scenario. The research aided in the application of the new technology of cloud computing adoption in the SMEs industry in India through the use of a wide range of criteria. The findings also help organizations study their information technology investments when applying cloud computing. 相似文献
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Ramesh Chandra Ritu Aneja Charu Rewal Swati Bhowmik Sujata K. Dass Ritu Jain 《Indian journal of clinical biochemistry : IJCB》2000,15(2):141-147
In the current study, we report perturbations in hepatic, renal and splenic heme synthesis at the level of the rate limiting
enzyme, δ-amino levulinic acid synthase (ALA-S) on ethanol administration independently and in conjunction with iron-protoporphyrin
(FePP) in male Wistar rats. Excessive ethanol administration (5 ml/kg bw) resulted in a significant induction of hepatic,
splenic and renal ALA-S activity. Simultaneous administration of FePP (50 μmol/kg bw) reverted the observed induction response
to a sharp decline. The features of the action of ethanol and FePP togetherin vivo, i.e. a substantial inhibition of ALA-S is suggestive of the beneficial effects of this formulation in acute attacks of porphyria. 相似文献
8.
Chavan VU Durgawale PP Sayyed AK Sontakke AV Attar NR Patel SB Patil SR Nilakhe SD 《Indian journal of clinical biochemistry : IJCB》2011,26(3):283-289
Twenty-four hour urinary albumin excretion (UAE) is considered as gold standard method for albuminuria measurement, but collection
of 24-h urine is inconvenient. The aim of present study was to evaluate whether albumin: creatinine ratio (ACR) and urinary
albumin concentration (UAC) in different spot urine samples correlate or not with 24-h UAE for screening of microalbuminuria
in type 2 diabetic patients. We collected first morning void (FMV), random urine sample (RUS) and 24-h urine, separately on
consecutive days from 104 type 2 diabetic patients. ACR and UAC in each spot urine sample compared with 24-h UAE with regard
to Pearson correlation coefficient. Pearson’s correlation of albumin: creatinine ratio (ACR) with 24-h UAE was (r = 0.802 and 0.623) in first morning void (FMV) and random urine sample (RUS), respectively. Pearson’s correlation coefficient
of urinary albumin concentration (UAC) compared with 24-h UAE was (r = 0.943 and 0.920), in FMV and RUS, respectively, P < 0.01. Results revealed that values in first morning void (FMV) were better correlated with 24-h urinary albumin excretion
(UAE), than the values in random urine sample (RUS). We conclude that the first morning void (FMV) may be able to replace
24-h urine collection, preferably urinary albumin concentration (UAC) in the initial screening of microalbuminuria in diabetic
patients. 相似文献
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E. Raji Nair Swati Banerjee Satish Kumar M. V. R. Reddy B. C. Harinath 《Indian journal of clinical biochemistry : IJCB》2001,16(1):132-135
Proteins secreted into the culture medium byMycobacterium tuberculosis (M. tb) are shown to be source of antigens of immunodiagnostic interest. Anin vitro released 31 kDa antigen ESAS-7F isolated fromM.tb H37Ra culture filtrate by salt precipitation, SDS-PAGE and cation exchange fast protein liquid chromatography (FPLC) was shown
earlier to be a diagnostically important antigen fraction. In this report, we describe the isolation of ESAS-7F antigen using
monospecific antibody coupled to sepharose CL-4B column. The percentage recovery of ESAS-7F antigen using affinity chromatography
was approximately 8% of the total ES antigen proteins compared to 0.05% obtained by conventional purification steps using
salt precipitation, SDS-PAGE and FPLC. Similar seroreactivity was observed by the antigen isolated by both the methods in
indirect ELISA. Affinity chromatography helped in an increased recovery of ESAS-7F antigen and obviates the need for time
consuming conventional purification steps. 相似文献