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隐孢子虫病免疫学研究现状 总被引:4,自引:0,他引:4
对隐孢子虫病病原的抗原成分,细胞免疫和体液免疫、免疫诊断及免疫防治进行了综述,为该病的有效防治提供参考。 相似文献
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Vashist SK Kaur I Bajpai RP Bharadwaj LM Tewari R Raiteri R 《Journal of Zhejiang University. Science. B》2006,7(9):683-685
We report an important observation that the surface conductivity of antibody layer immobilized on polylysine-coated glass
substrate decreases upon the formation of complex with their specific antigens. This change in conductivity has been observed
for both monoclonal and polyclonal antibodies. The conductance of monoclonal mouse IgG immobilized on polylysine-coated glass
substrate changed from 1.02×10−8 Ω−1 to 1.41×10−11 Ω−1 at 10 V when complex is formed due to the specific biomolecular interactions with rabbit anti-mouse IgG F(ab′)2. Similar behavior was observed when the same set up was tested in two clinical assays: (1) anti-Leishmania antigen polyclonal
antibodies taken from Kala Azar positive patient serum interacting with Leishmania promastigote antigen, and (2) anti-p21
polyclonal antibodies interacting with p21 antigen. The proposed concept can represent a new immunodiagnostic technique and
may have wide ranging applications in biosensors and nanobiotechnology too. 相似文献
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朱荫昌江苏省血吸虫病、寄生虫病防治研究所研究员,江苏省寄生虫分子生物学重点实验室主任,江苏省“135工程”寄生虫病学重点学科带头人,南京医科大学博士生导师,卫生部血吸虫病专家咨询委员会顾问,中国地方病协会血吸虫病专业委员会副主任委员《,中国血吸虫病防治杂志》常务副 相似文献
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对隐孢子虫病病原的抗原成分、细胞免疫和体液免疫、免疫诊断及免疫防治进行了综述,为该病的有效防治提供参考. 相似文献
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对近年伤寒免疫学诊断方法结合作者的研究工作进行了评价。伤寒免疫学诊断可分检测早期病人体液中抗原及测定患者血清中抗体两个方面。检测抗原以SPA-CoA与ELISA(包括Dot-ELISA)较为敏感和特异,而DRLAT则有简便的优点。测定抗体以PHA最为简便与快速。使用LPS-PHA可作伤寒的早期快速诊断,而Vi-PHA可作伤寒病后带菌者的初筛。ELISA有较PHA更高的敏感性,其中LPS-IgM-ELISA亦可作伤寒病人的早期诊断,而LPS-IgG-ELISA可用于该病的追溯诊断和血清流行病学调查。Vi-ELISA还可作伤寒病后带菌者初筛。不同条件实验室可根据取得试剂情况选择使用。 相似文献
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