同种异体肾移植26例报告

目的 总结26例肾移植的临床经验和教训。方法 回顾26例肾移植的临床资料，分析供受的状况，组织配型，移植手术，术后并发症及免疫抑制治疗方案对移植效果的影响。结果 70年代有80年代一年人／肾存活率均为33．3％／33．3％，90年代一年，三年人／肾存活率分别为90％／85％，80％／75％，超急性排斥反应发生率3．9％，急性及慢性排斥反应发生率均为11．5％，药物中毒发生率7．8％。结论 合理应用免疫抑制剂，提高外科手术，加强组织配型是提高肾移植受存活率的关键因素。     

组织金属蛋白酶抑制剂与大肠癌浸润转移的关系

基质金属蛋白酶家族是细胞外基质降解过程中的重要酶类,组织金属蛋白酶抑制剂因可抑制基质金属蛋白酶的活性而在肿瘤浸润转移中具有重要作用。     

乳酸链球菌和BF芽孢杆菌对抗菌素的反应

本选用链霉素，青霉素，头孢唑啉钠等抗菌素及苯酚作为抑制剂，在不同浓度，不同PH条件下分别进行试验试验，观察它们对乳酸链球菌和BF杆菌的反应。结果发现：在PH为7．0，链霉素的浓度为500μg/ml时，乳酸链球菌能正常生长，而BF杆菌生长受到抑制；青霉素浓度为100μg/ml时对乳酸链球菌的抑制作用相当小，对BF杆菌有较强的抑制作用；头孢唑啉钠浓度为500μg/ml时对乳酸链球菌的抑制作用较小，对BF杆菌有较强的抑制作用。本为乳酸链球菌和BF杆菌的分离和生产选择适合的抑制剂及PH条件提供了理论依据。     

溃疡性结肠炎的食疗方法

溃疡性结肠炎在临床表现为慢性腹泻、粘液便或粘液血便及腹痛,病程缓慢,轻重不一,有缓解和反复发作的趋势。其发病机制仍未明确,大多数学者认为,它是基因的易感性、肠道细菌、自身免疫反应及环境因素协同作用的结果。当前治疗仍以糖皮质激素类、柳氮磺胺类和免疫抑制剂等药物为主,这些治疗有副作用大,停药后易复发等缺点。现介绍几种结合中医药的食疗方法。柴胡桂枝饮配方:柴胡25克、人参10克、半夏20克、桂枝15克、黄芩10克、白芍10克、生姜10克、大枣10克、甘草6克、白糖20克制作:1·将以上药物洗干净,放入铝锅内,加水适量。2·将铝锅置武…     

Novel distribution pattern of fibrinolytic components in rabbit tissues extract： a preliminary study

Objective: The purpose of this work was to investigate the distribution pattern of fibrinolytic factors and their inhibitors in rabbit tissues. Methods: The components of the fibrinolytic system in extracts from a variety of rabbit tissues, including tissue plasminogen activator (tPA), plasminogen activator inhibitor-1 (PAI-1), plasminogen (Plg), plasmin (Pl) and α2 plasmin inhibitor (α2PI), were determined by colorimetric assay. Results: The tissue extracts in renal, small intestine, lung, brain and spleen demonstrated strong fibrinolytic function, in which high activity of tPA, Plg and Pl was manifested; whereas in skeletal muscle, tongue and stomach, higher activity of PAI-1 and α2PI showed obviously. Also excellent linear correlations were found between levels of tPA and PAI-1, Pl and α2PI, Plg and Pl. In related tissues, renal cortex and renal marrow showed distinctly higher activity of tPA and lower activity of PAI-1, with the levels of Plg and Pl in renal cortex being higher than those in renal marrow, where the α2PI level was higher than that in renal cortex. Similarly, the levels of tPA, Plg and Pl in small intestine were higher than those in large intestine, but with respect to PAI-1 and α2PI, the matter was reverse. In addition, the fibrinolytic activity in muscle tissue was lower, however, the levels of tPA, Plg, and Pl in cardiac muscle were obviously higher than those in skeletal muscles, and the levels of PAI-1 and α2PI were significantly lower than those in skeletal muscle. Conclusion: Our data demonstrate that a remarkable difference of the fibrinolytic patterns exists in rabbit tissues, which has probable profound significance in understanding the relationship between the function of haemostasis or thrombosis and the physiologic function in tissues.     

桦木酸磺酰胺衍生物类小分子Omicron融合抑制剂的综合性实验设计

新冠病毒刺突蛋白的S2介导了病毒与宿主细胞的膜融合过程,其氨基酸酸序列在Omicron等变异株中均高度保守,可作为开发新型抗新冠病毒药物的重要靶点。基于此前沿热点课题,该文设计了一类以桦木酸磺酰胺皂苷衍生物为结构骨架的新型小分子Omicron融合抑制剂的综合性实验。以马铃薯三糖桦木酸皂苷1为苗头化合物,利用生物电子等排体原理及基于结构的药物设计方法（SBDD）设计并合成了三个马铃薯三糖桦木酸磺酰胺衍生物T-1、T-2、T-3,利用NMR及HRESIMs对其进行结构表征,并基于S/HIV模型测试了其在细胞水平对Omicron等多种新冠病毒变异株的抑制活性。在此基础上,综合利用表面等离子共振SPR、免疫共沉淀（Co-PI）、细胞-细胞融合实验、分子对接等实验得出该类皂苷分子能够嵌入S1/S2亚基交界处空腔、稳定S蛋白融合前的构象从而抑制病毒入胞,进而表现出广谱抗新冠病毒活性的结论。     

枇杷果肉酶促褐变的研究——Ⅱ.酚酶的理化性质和对抑制剂的反应

在批把果肉酚酶提取分离的基础上,对酚酶的适宜pH条件和热稳定性以及对几种抑制物质的反应作了探索,结果表明酶A、酶B的最适pH范围分别为4.2～4.7和5.8～6.5,两种酶在pH6.5～7时较为稳定、酚酶具有较强的耐热性,当60～70℃时酚酶仍有较高的活力和较好的稳定性.抗坏血酸、苯式硫脲、亚硫酸氢钠、DT T(二硫赤苏糖酵)对酶A、B都有不同程度的抑制作用。0.05mmol/L铜试剂对酶B有显著的抑制作用,而对酶A的作用不明显,EDTA对酶B基本上无抑制作用。