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排序方式: 共有110条查询结果,搜索用时 15 毫秒
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Objective: A natural cyclic peptide previously isolated from Citrus medica was synthesized by coupling oftetrapep-tide units Boc-Leu-Pro-Trp-Leu-Ome and Boc-Ile-Ala-Ala-Gly-Ome after proper deprotection at carboxyl and amino terminals followed by cyclization of linear octapeptide segment.Methods: Solution phase technique was adopted for the synthesis of cyclooctapeptide--sarcodactylamide.Required tetrapeptide units were prepared by coupling of Boc-protected dipeptides viz.Boc-Leu-Pro-OH and Boc-Ile-Ala-OH with respective dipeptide methyl esters Trp-Leu-Ome and Ala-Gly-Ome.Cyclization of linear octapeptide unit was done by p-nitrophenyl ester method.The structure of synthesized cyclopolypeptide was elucidated by FTIR,1H NMR,13C NMR,FABMS spectral data and elemental analysis.The newly synthesized peptide was evaluated for dif-ferent pharmacological activities including antimicrobial,anthelmintic and cytotoxic activities.Results: Synthesis of sarcodac-tylamide was accomplished with >78% yield utilizing dicyclohexylcarbodiimide (DCC) as coupling agent.Newly synthesized peptide possessed potent cytotoxic activity against Dalton's lymphoma ascites (DLA) and Ehrlich's ascites carcinoma (EAC) cell lines,in addition to moderate anthelmintic activity against earthworms Megascoplex konkanensis,Pontoscotex corethruses and Eudrilus sp.Moreover,cyclopolypeptide displayed good antimicrobial activity against pathogenic fungi Candida albicans and Gram-negative bacteria Pseudomonas aeruginosa,in comparison to standard drugs griseofulvin and ciprofloxacin.Conclusion: Solution phase technique employing DCC and triethylamine (TEA) as base proved to be effective for the synthesis of natural cyclooctapeptide.N-methyl morpholine (NMM) was found to be a better base for the cyclization of linear octapeptide unit in comparison to TEA and pyridine. 相似文献
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采用PCR方法克隆获得拟穴青蟹抗菌肽scygonadin基因成熟肽片段,将其连接到Pichia pastoris酵母表达载体pPIC9K中,构建了分泌表达载体pPICgk-Scy.电击转化毕赤酵母GS115,经表型筛选和PCR鉴定证实了目的基因已稳定整合人酵母基因组中.以甲醇诱导表达阳性克隆,上清中表达产物经过Tricine-SDS-PAGE鉴定与预期的目的蛋白大小(约10ku)相符.并利用琼脂孔穴扩散法证明了表达产物能抑制革兰氏阳性菌和革兰氏阴性菌的生长. 相似文献
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BackgroundCecropin P1, acting as an antimicrobial, has a broad-spectrum antibacterial activity with some antiviral and antifungal properties. It is a promising natural alternative to antibiotics which is originally isolated from the pig intestinal parasitic nematode Ascaris suum. Many studies have shown that Cecropin P1 is helpful for the prevention or treatment of clinical diseases. Therefore, it is very necessary to establish a safe, nontoxic, and efficient expression method of Cecropin P1.ResultsThe results indicated that the recombinant protein was about 5.5 kDa showed by Tricine–SDS–PAGE and Western blot. And Cecropin P1 was efficiently secreted and expressed after 12 h of induction, with an increasing yield over the course of the induction. Its maximum concentration was 7.83 mg/L after concentration and purification. In addition, in vitro experiments demonstrated that Cecropin P1 not only exerted a strong inhibitory effect on Escherichia coli, Salmonella sp., Shigella sp., and Pasteurella sp., but also displayed an antiviral activity against PRRSV NADC30-Like strain.ConclusionsCollectively, the strategy of expressing Cecropin P1 in Saccharomyces cerevisiae is harmless, efficient, and safe for cells. In addition, the expressed Cecropin P1 has antiviral and antibacterial properties concurrently.How to cite: Jiang R, Zhang P, Wu X, et al., Expression of antimicrobial peptide Cecropin P1 in Saccharomyces cerevisiae and its antibacterial and antiviral activity in vitro. Electron J Biotechnol 2021;50. https://doi.org/10.1016/j.ejbt.2020.12.006 相似文献
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从柑橘的枝,叶,果实中分离培养出20株内生真菌,经形态特征鉴定它们分别为镰孢属(Fusarium sp.)、曲霉属(Aspergillus sp.)、毛霉属(Mucor sp.)、青霉属(Penicillium sp.)、葡萄孢属(Botrytis sp.)、盘多毛孢属(Pestalotia sp.)及3株不产孢子的未知菌株,各个菌属都具有较好的抗菌活性。 相似文献
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运动对心血管系统降钙素基因相关肽的影响 总被引:2,自引:0,他引:2
采用文献资料法,分析降钙素基因相关肽在心血管系统的分布及其对心血管系统的作用;探讨运动对心血管系统降钙素基因相关肽的影响;提出运动使心血管降钙素基因相关肽的表达发生改变,降钙素基因相关肽对运动性心肌肥大的发生、心肌收缩的增强,心肌细胞的保护等都起着重要作用。 相似文献
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大豆多肽作为生物活性肽的一种,具有很高的营养价值,补充大豆多肽对提高运动员机体的免疫力具有显著的效果;是否对大负荷训练后肠道免疫抑制具有调节作用,将成为今后研究的热点。 相似文献
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采用文献资料法,对降钙素基因相关肽(CGRP)及其与运动心脏重塑的研究进行回顾和总结。结果表明:心脏中存在CGRP阳性神经纤维及CGRP受体,这些神经纤维主要来自脊髓背根神经节细胞。通过对运动心脏重塑的机制、运动与CGRP的关系、CGRP的生理作用、CGRP的信号传导途径、细胞培养、分子生物学等方面的研究总结,认为CGRP是维持机体血流动力学稳定的重要代偿因子,是运动心脏重塑的重要介质。 相似文献
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运动对心脏内分泌功能的影响 总被引:6,自引:0,他引:6
王小燕 《广州体育学院学报》1997,(4)
本文对从事小长跑项目的男运动员(运动组)和未经过任何专项训练的男青年(对照组)各12名,进行了中等强度的急性动力性负荷实验,并于实验前后测试血浆心钠索,结果发现:运动后运动组和对照组对象的平均血浆心钠素水平均明显高于运动前(P<0.05,P<0.01);运动前后对照组对象的平均血浆心钠素量也分别明显高于运动组(P<0.05,P<0.01) 相似文献