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小西葫芦黄化花叶病毒(zucchini yellow mosaic virus,ZYMV)和黄瓜花叶病毒(cucumber mosaicvirus.CMV)是侵染葫芦科植物的主要病毒.本研究在三种供试品种的瓠瓜上单独和混合接种ZYMV和CMV,对各处理的病情和病毒积累量进行分析.两种病毒在三种瓠瓜供试品种上均表现明显的致病性.ZYMV和CMV的复合侵染加重瓠瓜发病症状,表现为协生作用.应用酶联免疫吸附法(ELISA)对三种瓠瓜上ZYMV与CMV进行检测并分析了病毒的积累量.结果表明,复合侵染情况下CMV的积累高于单独侵染,与致病性检测结果一致. 相似文献
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Yang Li Hang Yan Wu-jun Xue Pu-xun Tian Xiao-ming Ding Xiao-ming Pan Xin-shun Feng Xiao-hui Tian He-li Xiang Jun Hou 《Journal of Zhejiang University. Science. B》2009,10(11):820-828
Objective: To explore the effects of cytomegalovirus (CMV) infection on rejection-related gene expression in the endothelial cells of renal transplantation recipients. Methods: Endothelial cells (ECs) were cultured and stimulated by a variety of factors: A, normal control group; B, inactivated human cytomegalovirus (HCMV) infection group; C, HCMV infection group; D, HCMV supematant infection group; and E, ganciclovir HCMV group. Expression of intercellular adhesion molecule-1 (ICAM-1) and major histocompability complex (MHC) class Ⅰ and class Ⅱ antigens was detected by flow cytometry (FCM) and immuno-histochemistry. Results: We found characteristic CMV-infected ECs in this study. There were no significant differences among groups A, B and D (P>0.05). Although the expression levels of ICAM-1 were not significantly different between groups C and E (P>0.05), the ICAM-1 expression in these two groups was significantly higher than that in group A (P<0.05). ICAM-1 expression was detected in groups C and E, while there was no expression in groups A, B and D. Furthermore, there was no significant difference of ICAM-1 mRNA expression between groups C and E (P>0.05). Human leucocyte antigen (HLA)-ABC expression was detected in all the groups, while HLA-DR expression was only detected in groups C and E. There were no significant dif-ferences of HLA-ABC and HLA-DR expression among groups A, B and D (P>0.05). However, the HLA-ABC and HLA-DR expression levels in groups C and D were higher than those of the remaining groups previously reported (P<0.05). Meanwhile, the HLA-ABC and HLA-DR expression levels in group E were lower than those of group C (P<0.05). Conclusion: CMV could up-regulate the expression levels of ICAM-1 and MHC antigens, which was closely related to allograft rejection. 相似文献
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CMV甜瓜分离物外壳蛋白基因的克隆及其生物信息学分析 总被引:1,自引:0,他引:1
马新艳 《南阳师范学院学报》2008,7(12)
从河南省临颖县采集的病毒感染的甜瓜样本经ELISA检测和接种分离获得黄瓜花叶病毒(Cucumber mosaic vi-rus,CMV)分离物。把该分离物接种于西葫芦,从发病的叶片中提取总RNA,并以此为模板经RT-PCR扩增获得CMV的外壳蛋白(cp)基因,将其克隆到pUCm-T质粒上。经序列测定和生物信息学分析,结果表明该cp基因由657个核苷酸组成,编码218个氨基酸。预测该蛋白的等电点为5。12,分子量约为24kD。其核苷酸序列与黄瓜花叶病毒亚组I的分离物有较高的同源性,达92.2%~93.9%,与亚组II的同源性仅为76.8%~77.8%,与我国报道的CMV分离物的cp基因序列比较,只有香蕉株系XB外核苷酸序列的同源性达91.8%~93.4%。根据这些分析,该CMV分离物属于亚组I。另外还对黄瓜花叶病毒外壳蛋白的高级结构作出了预测。 相似文献
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