Poly A RNA status and expression of milli (m) and micro (μ) calpains in skeletal muscle of duchenne muscular dystrophy patients

Poly A RNA status and itsin vitro translation in a rabbit reticulolysate cell free system, were assessed in the skeletal muscle of young boys with Duchenne muscular dystrophy. Compared to normals there is a significant 48% increase in poly A RNA content of dystrophic muscle and its translatability was increased by 56% based on³⁵S methionine incorporation into total protein systhesised. Immunoprecipitation of the translated products with monospecific antibodies showed that there is a 2.6 fold and a 2 fold increase in m and μ calpains respectively. This underlines the importance of both synthetic and degenerative activities in the early pathology in DMD muscle.     

DMD系统的环形光栅的设计和制作

本文讨论了基于数字微镜系统（DMD）的环形光栅的设计和制作方法。利用DMD系统，我们可以制作出低成本、半径可调的环形光栅，为使其集成到微光机电系统提供了一种可能。通过实验，我们获得了最大外半径为42．7μm的环形光栅。     

LED显示屏的应用——电梯屏

利用单片机技术,把所要显示的内容以及DMD协议通过编程器写入单片机的存储器中.通过电脑通信RS-232,并按照DMD协议发送命令,调用单片机内部存储器的内容,再由单片机把数据传送到LED点阵上.显示屏上就能显示出通过电脑所调用的内容.本产品具有使用方便,性能稳定的特点.     

模板制备方法对单细胞PCR检测DMD基因的影响

旨在探讨模板制备方法对单细胞 PCR准确性的影响 ,采用 KOH/ DTT和液氮冻融煮沸两种不同方法裂解单个淋巴细胞 ,行 DMD基因外显子 17、19、4 4、4 5、4 8五重巢式 PCR.用 KOH/DTT法裂解的细胞扩增成功率为 98% ( 147/ 150 ) ,失败率为 2 % ( 3/ 150 ) ;用液氮冻融煮沸法裂解的细胞扩增成功率为 78% ( 117/ 150 ) ,失败率为 2 2 % ( 33/ 150 ) ,差异有显著性 (χ2 =2 8.4 ,P <0 .0 1) .结果表明单细胞 PCR模板制备与 PCR扩增失败有密切关系 ,选用合适的细胞裂解方法可提高单细胞 PCR的准确性     

Partial least squares based identification of Duchenne muscular dystrophy specific genes

Large-scale parallel gene expression analysis has provided a greater ease for investigating the underlying mechanisms of Duchenne muscular dystrophy (DMD). Previous studies typically implemented variance/regression analysis, which would be fundamentally flawed when unaccounted sources of variability in the arrays existed. Here we aim to identify genes that contribute to the pathology of DMD using partial least squares (PLS) based analysis. We carried out PLS-based analysis with two datasets downloaded from the Gene Expression Omnibus (GEO) database to identify genes contributing to the pathology of DMD. Except for the genes related to inflammation, muscle regeneration and extracellular matrix (ECM) modeling, we found some genes with high fold change, which have not been identified by previous studies, such as SRPX, GPNMB, SAT1, and LYZ. In addition, downregulation of the fatty acid metabolism pathway was found, which may be related to the progressive muscle wasting process. Our results provide a better understanding for the downstream mechanisms of DMD.     

多媒体投影机的比较与选购

目前,多媒体教学显示终端主要采用前投影机,本文重点介绍了新型DLP投影机的工作原理,并对LCD和DLP产品的优点、缺点和用途等进行了综合比较,最后介绍了选购投影机的一些注意事项。     

PWM理论及其在显示领域的应用研究

PWM技术广泛应用在通信,电网功率控制及变换等诸多领域,对模拟信号进行数字编码,能满足数字化系统中数据处理的要求。在数字光处理技术中,PWM用于显示技术中的灰度等级实现,本文着重讨论了数字微镜DMD显示中各种PWM方式实现原理及性能,提出了改进的DMD显示模式,在同等灰度等级下有效的减少了视频数据的传输带宽。     

领域特征词的提取方法研究

本文提出领域特征词(Domain Feature,DF)的概念,将其定义为:描述领域的最恰当的词语.由一个领域中所有领域特征词组成的集合称为领域特征词集(Domain Feature Set,DFs).为提高领域特征词集提取的效率和结果的客观性,本文给出一种以人机交互方式从自然语言语料库中提取的方法.该方法综合了长度优先切词算法和领域隶属度分析算法,前者保证召回率,后者提高准确率.实验证明,给定合适前景语料与背景语料,该方法能够显著提高领域特征词集构建的性能.领域特征词及其提取方法可以广泛地应用于信息和情报处理领域.