急性白血病相关基因的文本挖掘分析

从PubMed检索1966年到2005年9月6日间白血病与基因关系的相关文献3 529篇.经编程处理生成主题词词篇矩阵并进行聚类.通过聚类树图可将所提取的主题词/副主题词分成13类,经对比原始文献进行验证,全部29种基因中只与ALL相关的有3种, 占10.34%;只与AML相关的有8种,占27.59%.特异的可用于鉴别ALL和AML的基因有11种,占37.93%.通过主题词的共现关系进行聚类可以基本实现发现基因与疾病之间的联系,但该方法所获得的相关基因较少,不利于对疾病与基因关系的全面了解.     

Co-rumination and Immune Inflammatory Response in Healthy Young Adults: Associations with Interleukin-6 and C-Reactive Protein

A rich literature exists reinforcing the notion that both perceived and received social support has stress ameliorating and protective benefits both psychologically and physiologically. However, recent literature suggests that excessive dyadic negative problem talk about an issue, a phenomenon labeled co-rumination, may reverse much of the beneficial effects of social support. Healthy young adults participated in a laboratory research study exploring the associations between co-rumination and immune system inflammatory response. Partial correlations indicated a positive association between co-rumination and C-reactive protein and a negative association between co-rumination and interleukin-6 after controlling for stress, anxiety, and temperature. Discussion focuses on the means by which co-rumination may lead to negative health outcomes.     

拟南芥NHX基因密码子使用特性分析

运用CodonW程序对拟南芥NHX基因密码子组成、同义密码子使用频率和全长编码区密码子使用各项参数的计算和统计分析．结果表明：密码子适应指数（CAI值）与最优密码子使用频率（FOP）、密码子偏爱指数（CBI）均呈显著正相关（P〈0．05）;有效密码子数（ENC）与同义密码子第3位碱基含量（T3s）呈极显著负相关（P〈0．05）．拟南芥NHX基因RSCU值〉1的密码子多以A或T结尾．     

Two ancient rounds of polyploidy in rice genome

An ancient genome duplication (PPP1) that predates divergence ofthe cereals has recently been recognized. We report here another potentially older large-scale duplication (PPP2) event that predates monocot-dicot divergence in the genome of rice(Oryza saliva L.), as inferred from the age distribution of pairs of duplicate genes based on recent genome data for rice. Our results suggest that paleopolyploidy was widespread and played an important role in the evolution of rice.     

Enhancing cellular immune response to HBV M DNA vaccine in mice by codelivery of interleukin-18 recombinant

Objective: To investigate the effect of interleukin-18 (IL-18) on immune response induced by plasmid encoding hepatitis B virus middle protein antigen and to explore new strategies for prophylactic and therapeutic HBV DNA vaccines. Methods: BALB/c mice were immunized with pCMV-M alone or co-immunized with pcDNA3-18 and pCMV-M and then their sera were collected for analysing anti-HBsAg antibody by ELISA; splenocytes were isolated for detecting specific CTL response and cytokine assay in vitro. Results: The anti-HBs antibody level of mice co-immunized with pcDNA3-18 and pCMV-M was slightly higher than that of mice immunized with pCMV-M alone, but there was not significantly different (P>0.05). Compared with mice injected with pCMV-M, the specific CTL cytotoxity activity of mice immunized with pcDNA3-18 and pCMV-M was significantly enhanced (P<0.05) and the level of IFN-γ in supernatant of splenocytes cultured with HBsAg in vitro was significantly elevated (P<0.05) while the level of IL-4 had no significant difference (P>0.05). Conclusion: The plasmid encoding IL-18 together with HBV M gene DNA vaccines may enhance specific TH1 cells and CTL cellular immune response induced in mice, so that IL-18 is a promising immune adjuvant.     

免疫原理在网络考试系统安全中的应用

将基于人工免疫的IPS嵌入考试系统中,建立一种动态防御体系。文章主要介绍嵌入IPS的考试系统的五个组成部分及各部分如何协同工作。通过实验分别可以对未成熟检测器耐受期T、成熟检测器寿命L、匹配阈值β进行合理取值,使系统FP值较低,TP值较高,只有这样才能使系统性能最优化。     

补充谷氨酰胺及大强度训练对赛艇运动员细胞免疫机能影响的研究

目的观察8w大强度训练和补充谷氨酰胺对细胞免疫机能的影响,以期为赛艇运动员免疫机能的研究提供参考资料。方法采集陕西省赛艇队公开级二线16名男性运动员实验前后晨安静时静脉血5ml测T淋巴细胞亚群和IL-2。结果H组（谷氨酰胺＋大强度训练组）和C组（大强度训练组）运动员8周大强度训练后IL-2较自身安静时都降低,服用谷氨酰胺组（H组）外周血IL-2下降17．9％（P〉0．05）;C组下降48．5％（P〈0．001）;实验后H组CD3^＋无显著性变化,CD4^_上升7．6％（P〉0．05）,CD8^＋下降16．6％（P〈0．05）,CD4^＋／CD8^＋比值升高17．3％（P〈0．05）;C组CD3^＋下降（p〈0．05）,CD4^＋下降20．6％（P〈0．05）,CD8^＋无明显变化,CD4^＋／CD8^＋比值下降21．0％（P〈0．05）。结论8周大强度训练明显抑制赛艇运动员机体细胞免疫功能,补充谷氨酰胺8w可以提高赛艇运动员细胞免疫功能。     

A Review on the Role of Nutraceuticals as Simple as Se2+ to Complex Organic Molecules Such as Glycyrrhizin That Prevent as Well as Cure Diseases

Nutraceuticals are nutritional medicines which are present in edible food items. Most of them are antioxidants with various other biological properties viz, anti inflammatory, anti atherogenic, anticancer, anti viral, anti aging properties etc. They are as simple as minerals like Se²⁺ to complex organic molecules such as glycyrrhizin (Ca²⁺, K⁺ salts of glycyrrhizic acid). They can prevent as well as cure various diseases. Most of the medical people are not aware of the importance of the nutraceuticals as such matters are not part of their text books. Many still think that vitamins are the major nutritional medicines. Actually other dietary principles like terpenes, carotenes, phytosterols, polyphenols, flavanoids, di and poly sulphides, their sulfoxides and their precursor amino acids are necessary to scavenge free radicals in the body which are reactive oxygen species to protect and maintain the vitamin levels in the body. They down regulate the activities of those enzymes which are increased in diseases and they increase those that remove oxidants and detoxify carcinogens. They are immune boosters too. Recently glucosinolates, non toxic alkaloids, certain proteins and even fiber are included in the list of nutraceuticals.     

SIRTUIN1介导的NIH3T3成纤维细胞昼夜节律时钟基因表达抑制的光生物调节作用

背景和目的:昼夜节律是影响运动成绩的一个重要因素。研究发现,介导运动应激的肿瘤坏死因子(tumor necrosis factor,TNF)能够抑制昼夜节律时钟基因的表达。研究了低强度810nm激光(low intensity 810 nm laserir radiation,LIL)对TNF抑制效应的调节作用。材料和方法:50%马血清休克处理NIH3T3成纤维细胞2h,同步化昼夜节律时钟基因的表达后,加入10ng/mL TNF-alpha抑制它的表达,与此同时给予20min10mW/cm2的LIL照射。36h内,每隔6h检测细胞中时钟基因Clock、Bmal1、Per2、Dbp和烟酰胺腺嘌呤二核苷酸辅酶(nicotinamide adenine dinucleotide,NAD+)依赖的组蛋白去乙酰化酶1(sirtuin1,Sirt1)的mRNA的表达及胞内NAD+和其还原形式NADH的比值NAD+/NADH。结果:TNF-alpha分别在第12h和第18h抑制了Clock(P<0.05)、第18h抑制了Bmall(P<0.05)和Dbp(P<0.005)、第18h和第30h抑制了Per2(P<0.05)及...     

Transcriptomic Analysis of Aflatoxin B1-Regulated Genes in Rat Hepatic Epithelial Cells

Aflatoxins are the most popular hepatotoxicants. Chronic exposure to aflatoxins leads to a wide variety of liver diseases, such as hepatocellular carcinoma. In this study, we analyzed the genome wide expression profiles of aflatoxin B1-induced rat hepatic epithelial cells. The expression of 325, 184 and 199 special genes was altered when exposed to 0.03, 0.1 and 0.2 μmol/L aflatoxin B1 respectively, and 239 genes were commonly expressed. After the functional analysis on these dose-special genes, we determined several key pathways related to hepatotoxicity, such as TGF-beta signaling pathway, tight junction, adherens junction, the regulation of actin cytoskeleton, Erb B signaling pathway, p53 signaling pathway, pathways in cancer and axon guidance. Common genes were mainly associated with focal adhesion, ECM-receptor interaction, and cell adhesion molecules. Gene ontology annotations showed a good concordance with these pathways. The quantitative real-time polymerase chain reaction(PCR) analysis of selected genes showed similar patterns in microarrays. The toxicogenomic study provides a better understanding of molecular mechanisms of aflatoxins.