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系统比较了不同浓度的卡那霉素对普通烟草分化的影响,并测出了抑制普通烟草分化的卡那霉素的最低浓度。 相似文献
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Liu H Ding DL Jiang HY Wu XW Salvi R Sun H 《Journal of Zhejiang University. Science. B》2011,12(10):853-861
It is well known that ethacrynic acid (EA) can potentiate the ototoxicity of aminoglycoside antibiotics (AmAn) such as kanamycin
(KM), if they were applied at the same time. Currently, to create the model of EA-KM-induced cochlear lesion in rats, adult
rats received a single injection of EA (75 mg/kg, intravenous injection), or followed immediately by KM (500 mg/kg, intramuscular
injection). The hearing function was assessed by auditory brainstem response (ABR) measurement in response to click and/or
tone bursts at 4, 8, 12, 16, 20, 24, and 32 kHz. The static microcirculation status in the stria vascularis after a single
EA injection was evaluated with eosin staining. The pathological changes in cochlear and vestibular hair cells were also quantified
after co-administration of EA and KM. After a single EA injection, blood flow in vessels supplying the stria vascularis rapidly
diminished. However, the blood supply to the cochlear lateral wall partially recovered 5 h after EA treatment. Threshold changes
in ABR were basically parallel to the microcirculation changes in stria vascularis after single EA treatment. Importantly,
disposable co-administration of EA and KM resulted in a permanent hearing loss and severe damage to the cochlear hair cells,
but spared the vestibular hair cells. Since the cochlear lateral wall is the important part of the blood-cochlea barrier,
EA-induced anoxic damage to the epithelium of stria vascularis may enhance the entry of KM to the cochlea. Thus, experimental
animal model of selective cochlear damage with normal vestibular systems can be reliably created through co-administration
of EA and KM. 相似文献
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