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Benedicta D’Souza Rajeevalochana Parthasarathy Sreekantha Vivian D’Souza 《Indian journal of clinical biochemistry : IJCB》2011,26(4):396-399
The serum acid phosphatase (ACP) activity and Hemoglobin (Hb) levels were measured in malaria patients and nonmalarial fever
patients. The results were compared with normal healthy control subjects. ACP was significantly increased (P < 0.001) in all the malaria patients. ACP was significantly higher in Plasmodium falcifarum malaria and mixed malaria when compared to Plasmodium vivax malaria. Hb levels were significantly decreased in all the malaria patients which indicates that malaria parasite uses host
erythrocyte Hb as a major nutrient source. There is negative correlation(r = −0.478) between ACP and Hb in malaria patients, which is highly significant. These results suggest that the measurement
of ACP could be used as a marker for malaria. 相似文献
2.
Irene Poh‐Ai Cheong David Treagust Iorhemen J. Kyeleve Peck‐Yoke Oh 《International Journal of Science Education》2013,35(18):2497-2519
In this study, a two‐tier diagnostic test for understanding malaria was developed and administered to 314 Bruneian students in Year 12 and in a nursing diploma course. The validity, reliability, difficulty level, discriminant indices, and reading ability of the test were examined and found to be acceptable in terms of measuring students' understanding and identifying alternative conceptions with respect to malaria. Results showed that students' understanding of malaria was high for content, low for reasons, and limited and superficial for both content and reasons. The instrument revealed several common alternative conceptual understandings students' hold about malaria. The MalariaTT2 instrument developed could be used in classroom lessons for challenging alternative conceptions and enhancing conceptions of malaria. 相似文献
3.
Malaria infection is known to cause severe hemolysis due to production of abnormal RBCs and enhanced RBC destruction through apoptosis. Infected RBC lysis exposes uninfected RBC to the large amount of pro-oxidant molecules such as methemoglobin. Methemoglobin (MetHb) exposure dose dependently makes RBCs susceptible to osmotic stress and causes hemolysis. MetHb mediated oxidative stress in RBC correlated well with osmotic fragility and hemolysis. Interestingly, a reactive oxygen species (ROS) spike at 15 min was responsible for the observed effects on RBC cells. Two natural antioxidants N-acetyl cysteine and mannitol protected the RBC from MetHb-mediated defects, which clearly indicated involvement of oxidative stress in the process. MetHb due to its pseudo-peroxidase activity produces ROS in the external microenvironment. Therefore, classical peroxidase inhibitors were tested to probe peroxidase activity mediated ROS production with defects in RBCs. Clotrimazole (CLT), which irreversibly inactivates the MetHb (CLT-MetHb) and abolishes peroxidase activity, did not produce significant ROS outside RBC and was inefficient to cause osmotic fragility and hemolysis. Hence, initiating a chain reaction, MetHb released from ruptured RBC produces significant ROS in the external microenvironment to make RBC membrane leaky and enhanced hemolysis. Together data presented in the current work explored the role of MetHb in accelerated humorless during malaria which could be responsible for severe outcomes of pathological disorders. 相似文献
4.
Asaolu Modupe Fisayo 《Indian journal of clinical biochemistry : IJCB》2007,22(2):93-95
The plasma concentrations of total protein, albumin, immunoglobulins IgG, IgA and IgM and urinary protein were assayed in
250 pregnant Nigerian women with malaria and compared with 250 healthy pregnant women which served as controls. The mean values
of plasma total proteins, albumin, IgG and IgA were significantly lowered (P<0.05) while a slight increase in IgM was observed
in the malaria patients. Urinary proteins value of 23.10±0.50 mg/dl was obtained for the pregnant women with malaria, this
was significantly higher (P<0.05) than that of the controls with the corresponding value of 15.32±0.09 mg/dl. This study has
therefore demonstrated elevations of the urinary and decrease in plasma proteins in gestational malaria. These findings suggest
that the protein profile should be considered in the diagnosis and treatment of malaria. 相似文献
5.
M. M. Mya R. K. Saxena A. Roy D. N. Rao 《Indian journal of clinical biochemistry : IJCB》2004,19(1):88-92
Immunoreactivity properties of serum dilutions andPlasmodium falciparum malaria antigens were measured and compared by ELISA technique using different ELISA plates to evaluate the role of antigens
and serum dilutions for optimum binding. Also effort has been made to see the effect of reaction surface and material i.e.
ELISA plates for binding capacity. Serological properties were estimated by ELISA methods for detection of malaria and determination
of immunological characteristics. Three Pf antigens (PfAg) i.e. ring infected erythrocyte surface antigen: AR-1 (RESA), histidine-rich
protein 2 antigen (HRP-2) and glycophospholipid antigen (grown and developed Pf antigen from PSJ-M strain): GPL1 have been
used for serological testing of human blood samples by Enzyme Link Immunosorbant Assay (ELISA). 1∶100, 1∶1000 and 1∶10000
dilutions of Pf positive and negative serum (50 samples in each group) and 1∶1000 dilution of Pf antigens were used to measure
immunoreactive properties by ELISA method. Result of PfAg-serum immunoreactivity study showed that GPL1 has the highest degree
of immuno binding reactivity compared to other Pf antigens. HRP-2 and RESA antigens showed no significant difference to each
other. Study also found that Costar and Fastec ELISA plates have a better Ag−Ab binding capability compared to immulon and
Falcon plates at all dilutions of serum. Serum dilution of 1∶100 showed best binding and reactivity with Pf antigens followed
by 1∶1000 and 1∶10000 showed lowest reactivity. 相似文献
6.
A. A. Mahdi S. Ahmad R. K. Singh K. Jawad 《Indian journal of clinical biochemistry : IJCB》1994,9(1):35-36
The blood stage parasites fromin
vitro cultures ofPlasmodium
falciparum were employed for the detection of malaria antibodies in patient sera havingP.
vivax infections employing indirect fluorescent antibody (IFA) test. The antibody titres obtained for theP.
vivax infected sera ranged from 1∶128 to 1∶1024. Control slides smeared with non-immune sera did not show any fluorescence. On
the basis of the results of the present study it maybe concluded that antigen prepared fromin
vitro cultures ofP.
falciparum cross-reacts withP.
vivax antibodies. 相似文献
7.
A. G. Kulkarni A. N. Suryakar A. S. Sardeshmukh D. B. Rathi 《Indian journal of clinical biochemistry : IJCB》2003,18(2):136-149
Oxidative stress plays an important role in the development of malarial anemia. The present study was undertaken to study
the role of oxidant and antioxidants in the patients ofPlasmodium falciparum malaria (n=25),Plasmodium vivax malaria (n=25) as against the normal control subjects (n=25). The parameters included are the hematological [hemoglobin,
erythrocyte adenosine deaminase (ADA) activity, ADP-induced platelet aggregation] and serum total lipid peroxide as an index
of oxidative stress and antioxidants [erythrocytic superoxide dismutase (SOD) activity, serum vitamin E] & serum iron.
Significant alterations in all above parameters were noted in both groups of malaria patients as compared to control subjects.
Maximum significant alterations in hematological parameters were noticed inP. falciparum infection as compared toP. vivax malaria (p<0.001). Substantial rise in serum total lipid peroxides and a significant reduction in antioxidants such as serum
vitamin E and serum iron were noted inP. falciparum malaria as compared toP. vivax malaria (p<0.001), whereas maximum decline in erythrocytic SOD activity was observed inP. vivax infection as compared toP. falciparum malaria (p<0.05). Follow-up examination revealed the restoration of the levels of all biochemical parameters to the normal
level after 20 days of antimalarial therapy.
The study specified severity ofP. falciparum malaria and also functional duality of oxidant. 相似文献
8.
Prasannachandra Vivian D'Souza Benedicta D'Souza 《Indian journal of clinical biochemistry : IJCB》2006,21(2):103-106
Reactive oxygen species are thought to be involved in the pathogenesis of Malaria. To assess the extent of oxidative stress,
a study was conducted in patients withPlasmodium falciparum malaria andPlasmodium Vivax malaria. Plasma Thiobarbituric acid reactive substances (TBARS) were measured to assess the degree of lipid peroxidation.
Antioxidant status was measured by estimating the levels of Vitamins E and C. Results were compared with age and sex matched
control subjects. This study suggests that plasma TBARS levels were significantly increased in malaria patients. The patients
withP. falciparum infection showed significantly increased levels of lipid peroxides when compared toP. vivax malaria. The antioxidant Vitamins E and C were decreased significantly in malaria patients in both the groups. Maximum decline
in Vitamin C was observed inP. vivax malaria. Therefore it is been hypothesized that antioxidant Vitamins E and C could provide protection against the oxidative
stress induced by malaria. 相似文献
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