不同负荷运动训练对大鼠骨骼肌线粒体三羧酸循环的影响及其机制研究

目的：探讨不同负荷运动训练对大鼠骨骼肌线粒体三羧酸循环的影响及其机制。方法：将雄性Wistar大鼠50只随机均分为5组：安静对照组（C）、低负荷运动训练组（LT）、中等负荷运动训练组（MT）、高负荷运动训练组（HT）和极高负荷运动训练组（ST），每组10只。各运动组分别进行6周的跑台运动训练。训练方案结束后，取腓肠肌样本，提取线粒体，测定线粒体柠檬酸合成酶（CS）、异柠檬酸脱氢酶（ICD）和α-酮戊二酸脱氢酶（α-KGDHC）活性；线粒体Ga²⁺含量、胞浆NADH、NAD⁺、ATP和ADP含量，以及ICD mRNA转录水平。结果：（1）不同负荷运动训练组线粒体CS、ICD和α-KGDHC的活性均显著高于安静对照组（P < 0.01），且CS和ICD活性由高到低顺序均为：MT组 > HT组 > ST组 > LT组 > C组，α-KGDHC活性由高到低顺序为：HT组 > MT组 > ST组 > LT组 > C组。（2）不同负荷运动训练组线粒体Ca²⁺ 含量均显著高于安静对照组（P < 0.01），其含量由高到低顺序为：MT组 > HT组 > ST组 > LT组 > C组；胞浆NADH/NAD⁺和ATP/ADP的比值均显著低于安静对照组（P < 0.01），其比值由低到高顺序为：MT组 < ST组 < HT组 < LT组 < C组。（3）不同负荷运动训练组ICD mRNA转录水平均高于安静对照组（P < 0.01），其水平由高到低顺序为： MT组 > HT组 > ST组 > LT组 > C组。结论：低负荷、中等负荷、高负荷及极高负荷运动训练均可提高大鼠骨骼肌线粒体三羧酸循环功能，且中等负荷运动训练效果最佳。其机制与胞浆NADH/NAD⁺和ATP/ADP比值、线粒体摄钙能力及限速酶基因的表达有关。     

Identifying the combinations of motivations and emotions for creating satisfied users in SNSs: An fsQCA approach

Social Networking Sites (SNSs) play an important role in our daily lives and the number of their users increases regularly. To understand how users can be satisfied in the complex digital environment of SNSs, this study examines how motivations and emotions combine with each other to explain high satisfaction. Users’ motivations comprise four attributes, entertainment, information, social-psychological, and convenience. Emotions are divided into their two main categories, that is positive and negative emotions. We draw on complexity and configuration theories, present a conceptual model along with propositions and perform a fuzzy-set qualitative comparative analysis (fsQCA). Through an empirically study with 582 SNSs users, we present eight combinations (configurations) of motivations and emotions that lead to high satisfaction, which highlight the role of high convenience, followed by entertainment and information motivations in being satisfied with SNSs. High satisfaction can be achieved both when positive and negative emotions are high and low, depending on how they combine users’ motivations. None of the factors are indispensable to explain high satisfaction on their own, instead they are insufficient but necessary parts of the causal combinations that explain high satisfaction. This study contributes in SNSs literature by extending current knowledge on how motivations and emotions combine to increase satisfaction, and by identifying specific patterns of users for whom these factors are important and influence greatly their satisfaction.     

Reimagining Newsroom Collaboration: How Two European News Nonprofits Are Inviting Citizens In

ABSTRACT

News nonprofits in the U.S. have been proliferating over 15 years as a way of addressing troubles in the business model for news. For these newsrooms, collaboration, with each other and with mainstream news, has emerged as a key way to build readership and attain relevance in a crowded media space. Still, past research has told us that the strong connection to mainstream news has constrained these organizations’ critique of journalism. In Europe, nonprofit news remains nascent and represents a response to declining trust in and engagement with journalism, and rising populism across the continent. Against this very different context, this study examines two players at the forefront of the European news nonprofit movement. It demonstrates the path dependency inherent in the origins of these organizations: In Europe, they are a response to a different societal change, and thus developed rather differently than did their peers in the United States, with a focus on redefining the idea of collaboration and the role of their audiences by seeing citizens as collaborators, both in the creation and in the dissemination of news. By seeing citizens as collaborators, not just readers, they work to empower and build news audiences as well as participants.     

Proclaiming Library Worth in a Program Prioritization Process at a Mid-Sized Public University

Abstract

In an economic climate when colleges can no longer presume that legislators see institutions of higher learning as tacit public goods, institutions around the country are facing budget cuts. This article presents a case study of the experience of a mid-sized public state university and its Library in a budget crisis undergoing a university-wide program prioritization process. The authors review the process of writing three reports on the Library’s value, along with the approach to engaging staff in the process including a retreat. This article raises questions about what every library should consider assessing and provide tools for libraries navigating similar terrain.     

校园网管理信息系统中ASP技术应用

校园网络系统在学校的教学和管理工作中发挥着越来越重要的作用,本文从当前Internet/Intranet及MIS系统的发展出发,在比较当前流行的数据存取及信息处理技术基础上,阐述TASP的作用与优点及其在校园网信息处理中的应用。     

体育锻炼动机研究综述

动机是推动一个人进行活动的心理动因或内部动力。文章回顾了体育锻炼动机的的理论基础，介绍了体育锻炼动机的组成成分和常用的测量工具，从动机的年龄差异、性别差异、文化程度差异、经济收入差异、社会文化差异五个方面概括了以往相关研究的成果，并指出了目前研究中存在的问题。     

邢傲伟跳马侧手翻直体侧空翻转体810°的运动学分析

通过运动学等研究方法,揭示了我国优秀运动员邢傲伟完成的跳马侧手翻直体侧空翻转体810°的运动学规律与技术特点,对于进一步发展笠松类高难动作,提高我国男女跳马技术水平有促进作用.     

输煤控制系统硬件结构与上位机监控软件设计

根据工况将不同的设备组合成不同的工作流程，以集散控制技术为基础，应用PLC网络实现了对输煤设备的现场控制、电控室控制和中控室控制。并以FIX软件为工具，通过定义PLC的I/O端口映射表方法，以时间、事件、故障为触发手段，设计了实时监控软件，并实现了输煤量与数据库系统的数据接口。     

中国北方汉族青年ACE 基因I/ D多态性频率分布特征

通过分析与探讨汉族普通人群ACE基因I／D多态频率分布特征，试图为中国优秀耐力运动员的基因选材研究提供中国人相关人群ACE基因I／D多态频率分布的基础数据。选择18-22岁汉族学生216名作为研究对象，观察其ACE基因I／D多态频率分布。结果显示：北方汉族青年的ACE基因I／D多态频率分布，与国内相关研究结果一致，与欧美人群有非常显著差异。研究认为：从种族单一性、样本数量和年龄段的选择等因素考虑．研究结果不仅可以作为北方汉族青年ACE基因I／D多态频率分布的代表，而且对于杰出耐力的多态性标记研究来讲是理想的对照群体。