蛋白质组学研究的新进展

本文在对产生背景进行综述的基础上,着重介绍了蛋白质组学的研究技术、研究进展以及发展趋势。     

蛋白质组学研究的新进展

本文在对蛋白质组学概念、产生背景进行综述的基础上,着重介绍了蛋白质组学的研究技术、研究进展以及发展趋势。     

蛋白质组与蛋白质组学概述

本文对蛋白质组和蛋白质组学的概念、蛋白质组学的理论基础、研究技术及其应用作概述。     

Preparation of protein samples for gel electrophoresis by sequential extraction

Since preparation and solubilization of protein samples are crucial factors in proteome research, the authors established a sequential extraction technique to prepare protein samples from the body wall of the 5th instar larvae of silkworm,Bombyx mori. Two kinds of protein samples were obtained from the body wall using the method. Between the two types of samples only about 15% proteins were identical; the majority were different, indicating that more species of proteins could be obtained with the sequential extraction method; which will be useful for preparation of protein samples for proteome study.     

蛋白质组研究进展

本文介绍蛋白质组的概念形成、与基因组的区别、研究现状和研究方法.     

Comparative proteome analysis of Helicobacter pylori clinical strains by two-dimensional gel electrophoresis

Objective  

To investigate the pathogenic properties of Helicobacter pylori by comparing the proteome map of H. pylori clinical strains.     

金鱼雌核发育单倍体胚胎不同发育时期的差异蛋白质组学研究

为了进一步研究在金鱼雌核发育过程中不同发育阶段的单倍体蛋白质表达水平的差异,阐明单倍体不能正常发育的机制,利用金鱼雌核发育单倍体尾鳍出现期两个不同发育时期(W1,W2)的胚胎为材料,进行了比较蛋白质组学分析.结果鉴定到了一些与发育调控、神经发育、细胞分化等相关的12个差异蛋白质,并根据功能差异将其分为五种不同类型,这些差异蛋白质的鉴定为进一步阐明单倍体的发育异常机制奠定了良好的基础.