Segmenting the audience of a cause-related marketing viral campaign

This paper analyses the attitudinal effect of a cause-related marketing campaign which becomes viral through social networks. This attitudinal response is observed in three Internet user segments with different affinity levels: i) strong (familiar with the sponsoring brand and the promoted cause); ii) intermediate (familiar with the brand or the cause); and iii) weak (without previous experiences of either the brand or the social cause). To develop our experiment, 360 Internet surfers agree to participate. Their attitudes were measured before and after the showing of a viral spot in which a pet food brand encourages pet adoption. Our results show that a viral campaign works perfectly for the strong-affinity segment and adequately for the week affinity segment. On the contrary, this viral campaign leaves the intermediate-affinity segment indifferent.     

In vitro study on ribozyme against hepatitis C virus

Objective: To determine the effective nucleotide sites of ribozymes against HCV, and obtain a highly effective, nontoxic and inexpensive antisense ribozyme specific for HCV. Methods: Two effective ribozymes, targeted to HCV 5’NC region and C region, were designed and synthesized. Eukaryotic expression vectors, pSV2-gpt. CD-SR (, containing either HCRZNC or HCRZC were constructued and transfected into MT-2 cells, which had been infected by HCV. Quantitative PCR and hydridization were used to determine the effect of inhibition of HCV by ribozymes. Results: HCRZNC and HCRZC suppressed the replication of HCV by 54.7% and 62.1%, respectively. Furthermore, when both ribozymes were cotransfected into cells, they suppressed replication by 78.8%. Conclusion: Two specific antisense ribozymes have strong inhibitory effects on the replication of HCV in cultured cells, and have better effect when used together.     

一氧化氮合酶在病毒性肝炎中作用的研究进展

一氧化氮(NO)参与肝细胞的多项生理功能调节,参与病毒性肝炎的病理过程。一氧化氮合酶(NOS)为产生NO的限速酶,与NO的各种生理功能密切相关。本文综述了NOS基因在正常肝组织和病毒性肝炎的不同表达情况及其作用和意义。     

复方甘草酸苷联合拉米夫定治疗慢性乙型肝炎的临床研究

[目的]观察复方甘草酸苷对慢性乙型肝炎患者血清病毒学标志的影响．[方法]采用复方甘草酸苷联合拉米夫定治疗30例慢性乙肝患者,并与30例拉米夫定单药治疗患者比较,观察治疗后血清病毒学标志及肝功能指标的变化．[结果]治疗12周时联合治疗组血清HBeAg／抗-HBe转换率明显高于对照组；治疗12周和24周时联合治疗组肝功能指标复常率明显高于对照组．[结论]复方甘草酸苷治疗慢性乙型肝炎可能有助于患者在拉米夫定治疗过程中早期发生HBeAg／抗-HBe血清转换．     

2500例门诊患者肝功能及血清HBV感染指标检测结果分析

目的 :了解乙肝患者肝功能、乙肝五项及HBVDNA的变化及其相关性。方法 :收集门诊患者血清标本 ,同时检测肝功能、HBVELISA和HBVDNA三个不同水平。结果 :在收集的2 5 0 0例标本中 ,肝功能异常率为 6 2 .8% ,HBs -Ag阳性率为 71.2 % ,HBVDNA定量阳性率为 90 .4 %。当病毒拷贝数在 10 5～ 10 6之间时 ,肝功能异常率为 4 .2 85 % ,HBV拷贝数增加肝功能也不同程度加重 ;HBs -Ag阴性的标本中 ,FQ -PCR检出率为 30 .5 6 % ;单独HBs -Ab阳性的FQ -PCR检测的阳性率为 2 2 .35 %。结论 :HBVDNA的FQ -PCR是目前检测乙肝最为敏感的方法     

引力势能与太阳温度

根据引力理论和维里定理 ,对太阳温度做了简单的理论推导和估算     

Increased erythrocyte resistance to osmotic lysis in the acute hepatitis caused by true hepatotropic viruses non-A, non-B (nanb)

An unexpected increase in erythrocyte osmotic resistance during viral hepatitis in two patients prompted study of effects of hepatitis (in Karad) on osmotic resistance. The test was performed by placing erythrocytes in saline solutions of decreasing osmolarity and osmotic fragility defined in terms of the saline concentration at which hemolysis begins. Study included 44 infected subjects (acute viral hepatitis non-A non-B). All forty four infected subjects showed abnormal results when compared to normal subjects. increased erythrocyte osmotic resistance i.e. hemolysis begins between 0.45% to 0.40% of NaCl and is completed between 0.25% to 0.20% NaCl, whereas in normal subjects. hemolysis begins between 0.50% to 0.45% NaCl and is completed between 0.35% to 0.30% NaCl. Osmotic resistance was observed with increase in total bilirubin (mean±SD) (4.6 ±3.6), direct bilirubin (3.0±2.5), SGOT (58.8±55.5) SGPT (114.2±150.3) activity. Physiologic shifts in erythrocyte osmotic resistance may be due to changes in membrane lipid ratio.     

Clinical features of chronic hepatitis B patients with YMDD mutation after lamivudine therapy

INTRODUCTION Chronic hepatitis B(CHB)remains a major public health problem,affecting more than350mil-lion people worldwide.Cirrhosis,liver failure,or hepatocellular carcinoma will develop in approxi-mately15to40percent of infected patients(Ganem and Prince,2004).Lamivudine,an oral nucleoside analogue,inhibits HBV replication(de Clercq,2001;2004;Lai et al.,1997;1998;Marcellin et al.,2004).It can markedly reduce serum HBV DNA levels and normalize alanine aminotransferase(ALT)levels …     

人类新基因HBVDNAPTP1结合蛋白的生物信息学分析

[目的]应用生物信息学分析人类新基因乙型肝炎病毒(HBV)DNA聚合酶(Polym erase)反式调节蛋白(HBVDNAPTP1)结合蛋白(HBVDNAPTP1BP).[方法]利用生物信息学技术分析HBVDNAPTP1BP基因的染色体定位与组织表达,以及编码蛋白的化学物理性质与结构特征.[结果]HBVDNAPTP1BP基因染色体定位于1号染色体短臂3区5带1亚带,可在组织中低表达,但在多个组织中无表达.HBVD-NAPTP1BP的相对分子量为11 905.6,理论pI为7.72,不同条件下的消光系数为14 230 M-1cm-1或13 980M-1cm-1(280 nm),在体外哺乳动物网状细胞中的半寿期为30 h,并且无卷曲螺旋区域,但具有3个较强的疏水区域.HBVDNAPTP1BP无特殊二级结构,仅有1个蛋白激酶C磷酸化位点,不具有跨膜螺旋结构,无信号肽序列,定位于细胞核中.[结论]应用生物信息学对HBVDNAPTP1BP进行了分析,为进一步研究其生物学功能及其在乙型肝炎、肝细胞癌中的作用机制提供了依据和线索.     

病毒性肝炎213例胆囊声像图变化的临床分析

本文对病毒性肝炎２１３例胆囊声像图变化进行分析，初步探讨了病毒性肝炎胆囊改变发生机制。