On-line estimation of concentration parameters in fermentation processes

INTRODUCTION Saccharomycete, closely linked with human life, is the only microbe that has been utilized to the extent of over a million tons in the world, and is one of the modelled biological bacteria mostly used in modern genetic engineering. Since there are no effective online instruments for measuring the production or resultant concentration, which is a very important quality index in fermentation process, soft sensors (Martin, 1997; Tham et al., 1991; Assis and Filho, 2000; Salgad…     

Immunomodulatory function of orally administered thymosin α1

Objective: To investigate the immunological function of a yeast expression system for thymosin α1(Tα1). Methods: A constructed Tα1 yeast expression system was used to investigate the immunological function of orally administered Tα1. Dried yeast containing three different concentration of Tα1 was fed to normal Balb/c mice and other Balb/c mice whose immunities were inhibited in advance by cyclophosphamide. Synthesized Tα1 peptide was used as positive control and dried yeast with empty plasmid was used as negative control. CD4 and CD8 levels were detected by flow cytometry assay. TNF-α IFN-γ IL-2, IL-6 and IL-10 levels were detected by liquid chip. Results: In normal Balb/c mice or immune inhibition Balb/c mice, CD8 levels were significantly increased. Especially in immune inhibition Balb/c mice, CD8 levels in synthesized Tα1 group (18.77％±4.72％),small dose group (13.48％±6.17％) and large dose group (22.74％±1.09％) were significantly higher than that in empty yeast control group (7.49％±2.14％). Conclusion: Orally administered Tα1 has its certain immunomodulatory function.     

浓香型大曲酒窖池养护探讨

窖池是浓香型大曲酒生产企业最重要的设备,本文阐述了窖池养护的重要性,分析了窖池养护的必要性和生物学基础,提出了窖池养护的原则,探讨了窖池养护的措施．     

高赖氨酸酵母菌的辐照选育

采用高能电子束辐照处理四株不同的酵母菌株 ,筛选赖氨酸含量较高的突变株并研究电子束对酵母的辐照效应 .试验表明 :电子束处理酵母的最佳剂量为 4 .14～ 4 .83k Gy,临界致死剂量为6.8k Gy,起始剂量为 0 .69k Gy,不同菌株对电子束的敏感性不同 ,其敏感性强度分别为产朊假丝酵母 >产香酵母 >热带假丝酵母 >面包酵母 .发酵试验表明 :不同剂量的电子束处理酵母 ,其胞外赖氨酸含量都发生不同程度的变异 ;其变异幅度为 84～ 2 90μg/ ml,经电子束处理的酵母其胞外赖氨酸含量比出发菌株赖氨酸含量平均提高 10 .9% .在剂量为 4 .83k Gy,致死率 80 %的条件下 ,酵母胞外赖氨酸变异幅度最广 ,产生高赖氨酸菌株的突变株比例为最大 ,达到 16.5% (胞外赖氨酸含量在 1.4 mg/ ml以上 ) ,在突变体选育方面具有较大的可选择性和可行性 ,为该菌的菌种改良提供了一条遗传育种的途径     

强聚硒酵母发酵罐扩大培养条件的优化

在前期研究的基础上,进行了强聚硒酵母发酵罐扩大培养条件的优化,最终确定发酵罐中最适发酵条件：麦芽汁1%,初始硒浓度为15μg/mL,在其发酵进入到对数生长期后再添加亚硒酸钠使之硒浓度达到25μg/mL,种子培养时间12 h,接种量为10%,培养温度28℃,通气量6 L/h,搅拌速度150 r/min,培养时间29 h。在此最优化条件下,酵母生物量达到8.05 g/L,硒含量达到1 003.19μg/g。     

生物发酵对小麦麸皮营养成分的影响

试验利用发面酵母通过在不同时间、温度等条件下对小麦麸皮进行生物发酵,并对发酵后的麸皮进行营养成分测定.结果表明:发酵后的麸皮营养成分较原样均有极显著的改变(P＜0.01),当温度为35℃、温度为58%、麸皮发酵时间为18小时(h)时,粗蛋白含量提高了119.59%,乙醚浸提物含量提高了73.68%.     

利用酵母菌处理高浓度有机废水研究

对酵母菌在高浓度有机废水处理中的作用进行阐述,总结酵母菌处理技术的研究现状,对几种难处理高浓度有机废水(味精废水、橄榄油厂废水、皂素废水、果汁废水、木薯酒精废液)的酵母菌处理技术进行概括。酵母菌处理技术具有一定的经济效益与社会效益,发展前景良好。     

酵母富集微量元素的研究

酵母菌含有丰富的营养成分,并能将无机态微量元素转化为有机态微量元素。在此简要介绍了酵母菌富集微量元素的生理功效、研究方面的进展及功能性酵母的研究方法。