通经草总黄酮含量及抗炎作用研究

以70%乙醇为提取溶剂,超声提取温度为60℃,料液比为1:30,采用先浸泡再水浴加热后超声的方法提取通经草不同部位的总黄酮,然后通过小鼠耳肿胀实验,比较通经草不同部位的抗炎作用。结果表明,通经草根中总黄酮的含量为2.37%,茎中总黄酮的含量为3.05%,叶中总黄酮的含量为5.10%。通经草不同部位高、中剂量提取液均能显著抑制二甲苯致小鼠耳廓肿胀,与阴性对照组比较有显著性差异(P<0.01)。表明通经草有显著的抗炎作用,抗炎作用强弱依次为叶>茎>根。     

天南星提取物的抗炎作用及机制研究

目的：观察天南星提取物的抗炎作用并分析其机制。方法：采用二甲苯致小鼠耳廓肿胀、棉球诱发小鼠肉芽肿增生及醋酸致小鼠腹腔毛细血管通透性增加等三种模型,观察天南星提取物对不同炎症模型的抗炎作用。结果：天南星块茎醇提取物、乙酸乙酯提取物,天南星果实石油醚提取物均能明显抑制二甲苯致小鼠耳廓肿胀度（P〈0.01）;能减轻小鼠棉球肉芽肿（P〈0．01）;且能明显降低小鼠毛细血管通透性（P〈0．01,P〈0.05）。结论：天南星上述提取物具有明显的抗炎作用,其抗炎作用可能与降低血管通透性有关。本实验为其开发利用提供了科学的依据。     

白藜芦醇/富勒烯纳米组装体的制备——在胆管支架抗炎涂层中的应用评估

采用白藜芦醇(Res)和富勒烯(C60)结合制备Res/C60纳米组装体并验证其安全性和抗炎性。通过扫描电子显微镜(SEM)、拉曼光谱和X射线衍射研究了纳米组装体形貌、粒径大小及波谱峰值特征。其次,通过紫外分光光度法研究了Res的药物缓释行为。最后,通过MTT法在人脐静脉内皮细胞(HUVECs)层面验证了Res/C60纳米组装体的安全性以及体外的抗炎性。研究发现,Res/C60纳米组装体呈短棒状,横截面平均直径为(379.74±33.47)nm。拉曼光谱和X射线衍射特征峰显示Res/C60纳米组装体被成功制备。Res/C60纳米组装体的聚氨酯(PU)涂层中Res药物在30 d内累积释放量可达60%左右。胆管支架涂层对HUVECs细胞毒性较低,且通过释放Res可显著抑制炎症因子的释放。     

哮喘宁颗粒抗炎、免疫作用实验研究

观察哮喘宁颗粒的抗炎、免疫作用。方法:用二甲苯及琼脂建立炎症模型观察哮喘宁颗粒对急性炎症及慢性炎症的作用;从体液免疫和细胞免疫两方面观察哮喘宁颗粒的免疫作用。结果:哮喘宁颗粒可明显对抗急性炎症和慢性炎症,对体液免疫和细胞免疫具有调节作用。结论:哮喘宁颗粒有较好的抗炎和免疫调节的作用。     

竹节三七总皂苷抗炎动物模型的研究

目的：建立竹节三七的抗炎动物模型,寻找最佳药效时间,检验其有效成分竹节三七总皂苷（total saponins of Panax japonicus,TSPJ）抗炎效果,为临床研究提供依据。方法：采用小鼠巴豆油耳肿胀实验,观察TSPJ不同时间、不同剂量的抗炎效果。结果：TSPJ（25,50,100mg.kg-1）灌胃能显著抑制巴豆油所致的小鼠耳肿胀,其作用与剂量呈正相关,其在给药后4小时达到最佳效果。结论：TSPJ具有明显的抗炎作用,与对照组比较差异非常显著。     

抑郁症与炎症

抑郁症（depression）是一种以情绪低落、悲观厌世、认知和睡眠障碍为主要特征的情感性精神障碍,临床一线抗抑郁药主要是基于单胺假说的药物,通过对单胺递质的调节发挥抗抑郁作用,尽管疗效肯定,但普遍存在起效延迟、有效率低、不良反应多等诸多缺憾,单胺假说也不能解释抑郁症的全部现象。因此,近年来除单胺假说外的其它假说越来越引起重视,炎症假说认为应激刺激引发炎症过程,最终导致抑郁症的发生,该文着重通过抑郁症的（神经）炎症假说阐述抑郁症与炎症的关系。     

扁担杆正丁醇提取物的抗炎镇痛作用

应用化学方法制备扁担杆正丁醇提取物。采用热板法观察提取物对小鼠的镇痛作用,用二甲苯致小鼠耳郭肿胀法观察其抗炎作用。结果显示高浓度扁担杆正丁醇提取物有明显镇痛作用,并且能明显抑制二甲苯引起的小鼠耳郭肿胀。     

哌嗪的氨基酸缀合物的合成、表征及抑菌活性研究

哌嗪是化工生产的常用化合物,也是重要的医药中间体。近些年来,哌嗪类衍生物作为药物的研发引起了人们的广泛关注。本文以哌嗪为起始原料,将4种氨基被保护的氨基酸导入哌嗪的N-1和N-4位,再脱去保护基,合成了8种哌嗪的氨基酸缀合物,并通过IR和1H NMR对所合成的氨基酸缀合物进行了结构表征。然后对目标产物进行了野生型大肠杆菌(Escherichia coli)和金黄色葡萄球菌(Staphylococcus aureus)抑菌活性测试。结果表明化合物PP-1a、PP-2a、PP-1b、PP-2b、PP-1c、PP-2c、PP-2d对Staphylococcus aureus具有一定的抑菌效果并且抑菌作用随化合物浓度的升高而增强。     

The isolated and combined effects of selected physical activity and ibuprofen on delayed-onset muscle soreness

Delayed-onset muscle soreness refers to the skeletal muscle pain that is experienced following eccentric exercise. The aim of the present study was to examine the physiological effects of physical activity with or without ibuprofen on delayed onset muscle soreness. Forty-four non-athletic male volunteers (age 24.3?±?2.4 years) were randomly assigned to one of four groups: physical activity (n = 11), ibuprofen (n = 11), physical activity and ibuprofen (combination, n = 11), or control (n = 11). The physical activity programme comprised 5?min of walking and jogging, 10?min of static stretching of the hands and shoulder girdle, and 5?min of concentric movements with sub-maximal contractions. The total amount of ibuprofen consumed by a single individual was 2800?mg; this was taken from 1?h before the eccentric actions up to 48?h after it. Delayed onset muscle soreness was induced by performing 70 eccentric contractions of the biceps muscle of the non-dominant side on a modified arm curl machine. Perceived muscle soreness, maximal eccentric contraction, creatine kinase enzyme activity and elbow range of motion were assessed 1?h before and 1, 24 and 48?h after the eccentric actions. The results indicated that, after the eccentric actions, soreness increased (P?<?0.001) across time in all groups, with the highest values being recorded at 24?h. At 24 and 48?h, greater soreness (P <?0.001) was observed in the control group than in the physical activity and combination groups. After the eccentric actions, creatine kinase increased and was elevated (P?<?0.001) compared with baseline in all groups, with values returning to baseline in the physical activity and combination groups by 48?h. However, creatine kinase in the control and ibuprofen groups was still significantly higher than at baseline after 48?h. Creatine kinase was higher (P?<?0.001) in the control group than in physical activity and combination groups at 24 and 48?h. There was also a reduction (P?<?0.001) in elbow range of motion across time. This reduction in elbow range of motion was greater (P?<?0.001) in the control and ibuprofen groups than in the physical activity and combination groups at 1, 24 and 48?h. The reduction in maximum eccentric contraction was greater (P?<?0.001) in the control and ibuprofen groups than in the physical activity group at 24 and 48?h and the combination group at 48?h. In conclusion, the results add to our understanding of the effects of physical activity and the combination of physical activity and ibuprofen in reducing the severity of muscle soreness induced by eccentric exercise. Physical activity conducted before eccentric exercise alleviates muscle soreness. Our results indicate that physical activity with or without ibuprofen helps to prevent delayed-onset muscle soreness.     

吲哚美辛对小鼠肾缺血再灌注损伤的保护作用(英文)

研究目的:在小鼠模型中利用吲哚美辛阻断COX-1/2通路,探讨非甾体类抗炎药对肾缺血再灌注损伤的保护作用。创新要点:非甾体类抗炎药被认为具有肾毒性,本研究首次在小鼠模型中探讨非甾体类抗炎药对肾缺血再灌注损伤的保护作用。研究方法:小鼠左侧肾蒂夹闭后,通过腹腔注射不同剂量的吲哚美辛,在肾缺血再灌注损伤24小时后,获取血液和肾脏标本。利用酶联免疫(ELISA)试剂盒测定血清肌酐和细胞因子浓度来评估肾功能,肾组织样本进行苏木精-伊红染色和免疫组化分析。重要结论:腹腔注射吲哚美辛5 mg/kg组的小鼠血清肌酐值与对照组相比显著降低,肾小管损伤也显著减轻(见图1和2);腹腔注射5和7 mg/kg吲哚美辛组的小鼠血清肾肿瘤坏死因子-α和白介素-6的浓度显著降低(见图3a和3b);腹腔注射3和5 mg/kg吲哚美辛组的小鼠血清血栓素B2与6-酮前列腺素F1α的比值明显降低(见图3c);腹腔注射5 mg/kg吲哚美辛组小鼠肾组织COX-1和COX-2染色较弱(见图4)。因此,吲哚美辛对小鼠肾缺血再灌注损伤的作用与其剂量相关,在某个特定的剂量范围内具有肾保护作用。吲哚美辛对小鼠肾缺血再灌注损伤的保护作用与阻断COX-1/2有关。