化学生物学的发展与展望

化学生物学是一门新兴的交叉学科,其中利用小分子调节剂探索生物过程是该研究领域的核心。此外,生物催化和生物转化、分子模拟和识别以及生命体系中分析方法的研究等方面也是该学科的重要组成部分。     

Traceless enzymatic protein synthesis without ligation sites constraint

Protein synthesis and semisynthesis offer immense promise for life sciences and have impacted pharmaceutical innovation. The absence of a generally applicable method for traceless peptide conjugation with a flexible choice of junction sites remains a bottleneck for accessing many important synthetic targets, however. Here we introduce the PALME (protein activation and ligation with multiple enzymes) platform designed for sequence-unconstrained synthesis and modification of biomacromolecules. The upstream activating modules accept and process easily accessible synthetic peptides and recombinant proteins, avoiding the challenges associated with preparation and manipulation of activated peptide substrates. Cooperatively, the downstream coupling module provides comprehensive solutions for sequential peptide condensation, cyclization and protein N/C-terminal or internal functionalization. The practical utility of this methodology is demonstrated by synthesizing a series of bioactive targets ranging from pharmaceutical ingredients to synthetically challenging proteins. The modular PALME platform exhibits unprecedentedly broad accessibility for traceless protein synthesis and functionalization, and holds enormous potential to extend the scope of protein chemistry and synthetic biology.     

生物催化反应在工业生产中的应用

本文通过生物催化反应的几种类型简单介绍了生物催化剂－酶应用于工业生产的情况。     

微生物细胞生物催化3-羰基丁酸乙酯制备光学活性3-羟基丁酸乙酯的研究进展

综述了当前国内外在以微生物活细胞生物催化制备光学活性3-羟基丁酸乙酯(EHB)的研究进展,重点介绍了各种提高微生物活细胞催化不对称合成反应立体选择性的方法:反应物的浓度、辅助底物和添加剂的选择、基因工程等。最后对生物催化制备光学活性EHB的工业化前景进行了展望。     

离子液体的生物相容性及在生物催化反应中的应用

离子液体作为一种新型的生物催化反应介质,与传统有机溶剂相比,表现出独特的性质和现象,引起了人们的广泛兴趣.本文从离子液体对微生物细胞的毒性及离子液体中酶的特性阐述离子液体的生物相容性;按照不同反应体系综述了离子液体介质中生物催化反应的研究进展,包括离子液体/超临界CO2双相体系,疏水性离子液体/水两相体系,以及含亲水性...